EMAGE mouse gene expression spatial database

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EMAGE:12328

Ssrp1 structure specific recognition protein 1 ( MGI:107912)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007091. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007091_01	euxassay_007091_02	euxassay_007091_03	euxassay_007091_04

euxassay_007091_05	euxassay_007091_06	euxassay_007091_07	euxassay_007091_08	euxassay_007091_09

euxassay_007091_10	euxassay_007091_11	euxassay_007091_12	euxassay_007091_13	euxassay_007091_14

euxassay_007091_15	euxassay_007091_16	euxassay_007091_17	euxassay_007091_18	euxassay_007091_19

euxassay_007091_20	euxassay_007091_21	euxassay_007091_22	euxassay_007091_23	euxassay_007091_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12328_wholemount_strong.wlz

12328_wholemount_moderate.wlz

12328_wholemount_weak.wlz

12328_wholemount_possible.wlz

12328_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12328_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4627
Entity Detected:	Ssrp1, structure specific recognition protein 1 ( MGI:107912)
Sequence:	sense strand is shown >T4627 GCGTCCCCCTGCGCCCACCGCCCTCACTGTGCCGTCGCGAGGCTGCAGCTCTTAGAGATTCCCACCCTGC TGGACATCTACACAACTAACCAGGAATTGGGGCACAGCATCCAGAGAAGGGTTCGTTTTCTCTGGTGCAG CCTAGGGCTGTGTGCTCAGGCCACCACGGGCAGGTGAACATGGCAGAGACATTGGAGTTCAACGACATCT TCCAGGAGGTGAAAGGGTCCATGAATGATGGGAGGCTTCGATTGAGCCGTCAGGGTATCATCTTTAAGAA CAGCAAGACGGGCAAAGTGGACAACATCCAGGCTGGGGAGTTGACAGAAGGCATCTGGCGTCGGGTAGCA TTAGGCCATGGGCTTAAACTGCTCACAAAGAATGGGCATGTCTACAAGTACGATGGCTTCCGCGAATCGG AGTTTGAGAAACTCTCTGACTTCTTCAAAACTCACTATCGCCTTGAGCTAATGGAGAAGGATCTGTGTGT GAAGGGCTGGAACTGGGGGACAGTGAAGTTTGGAGGACAGCTGCTTTCTTTTGACATTGGTGATCAACCA GTCTTTGAGATACCCCTAAGCAATGTGTCCCAGTGTACCACAGGCAAGAATGAGGTGACCCTGGAATTCC ACCAGAATGACGATGCTGAAGTATCTCTCATGGAGGTGCGCTTCTATGTTCCTCCCACGCAGGAAGATGG TGTGGACCCTGTGGAGGCCTTTGCCCAGAATGTTCTGTCAAAGGCAGATGTGATCCAGGCCACCGGAGAC GCCATCTGCATCTTCCGGGAGCTGCAGTGTTTGACTCCTCGCGGTCGATACGATATCCGGATCTACCCTA CCTTTCTACACCTGCATGGCAAGACCTTTGACTACAAGATCCCCTATACTACAGTTCTCCGTCTCTTCCT GCTACCACACAAGGATCAGAGACAGATGTTCTTTGTGATCAGCTTGGATCCTCCCATCAAGCAGGGCCAA ACTCGTTACCACTTCCTGATCCTCCTCTTCTCCAAGGATGAGGACATCTCCTTGACTCTCAACATGAATG AGGAAGAAGTAGAAAAGCGCTTTGAGGGGCGACTCACCAAGAACATGTCAGGATCCCTCTATGAAATGGT CAGTCGGGTCATGAAAGCACTTGTCAACCGTAAAATCACAGTCCCAGGCAACTTCCAAGGGCACTCAGGG GCCCAGTGTATTACCTGCTCCTATAAGGCCAGCTCAGGACTCCTGTACCCACTGGAGCGGGGCTTCATCT ACGTGCATAAGCCCCCTGTGCACATCCGCTTTGATGAGATCTCTTTTGTCAACTTTGCCCGTGGCACCAC GACCACTCGTTCCTTCGACTTTGAGATTGAGACCAAGCAAGGCACTCAGTATACCTTCAGCAGCATTGAA AGGGAGGAGTATGGAAAGCTTTTTGATTTTGTCAATGCGAAAAAGCTCAACATCAAGAACAGAGGACTGA AAGAGGGCATTAACCCAGGCTATGACGATTATGCTGACTCTGATGAAGACCAGCATGATGCCTATTTGGA GAGGATGAAGGAGGAGGGCAAGATCCGGGAGGAGAATGCCAATGACAGCAGCGACGACTCAGGAGAAGAG ACTGATGAGTCCTTCAATCCTGGTGAAGAAGAAGAAGATGTGGCAGAGGAGTTTGACAGCAATGCCTCTG CCAGCTCCTCCAGCAATGAGGGTGACAGTGACCGTGAAGAGAAGAAACGGGAACAGCTCAAAAGGGCTAA GATGGCCAAGGATCGAAAGAGCCGCAGGAAGTCTTCAGAGGCAAAGAAGGGTAAAGATCCAAACGCCCCA AAGAGGCCTATGTCTGCGTACATGCTGTGGCTTAATGCAAGCCGCGAGAAGATCAAGTCGGATCATCCTG GCATCAGTATCACAGATCTTTCCAAGAAGGCAGGGGAAATCTGGAAGGGAATGTCCAAAGAGAAGAAAGA GGAGTGGGACCGCAAGGCTGAGGATGCTAGGAGGGAGTATGAGAAAGCCATGAAAGAGTATGAAGGAGGC AGAGGGGACTCGTCTAAAAGGGACAAGTCTAAGAGGGACAAGTCTAAGAAGAAAAAGAAAGTAAAAGCAA AGATGGAAAAAAAGTCCACTCCTTCCCGGGGCTCGTCATCCAAGTCTTCATCCAGGCAGTTGAGTGACAG CTTCAAGAGCAAAGAGTTTGTGTCCAGTGATGAGAGCTCTTCAGGCGAGAACAAGAGCAAAAAGAAGAGG AGGCGGAGCGAGGACTCTGAAGAGGAGCTAGCCAGTACCCCCTCCAAGCTCAGAGGACTCTGCCTCGGGA TCTGATGAATAAAGGAGGGAATTCCCACCCCGTCACAGCTCCAGTCTCTCACATAGTCCTTGGATTCTGT GCCATCTGAGTAACTGCTCTTGGTGGCTTCCACTGCCCTGAGGCTTTGAGGGAAGCACCTAACCTCTGCT GCTTTGCAAGGAAGGCTCCTTGCAATCTGGAGAGAGACTCGGTAGGAGTGTGTTGTCTTCTACTCGCAGT GCATTGAAGGACCCAAGTCCTCAGCCTACTTTCCTACTTTCTGACTGTAGTAAAAGCTGCTTCCTGTCCT GTTCAAGTTGCTGCAGCAGGGGTCATGCCAAGTCAGGCCTGTAGCTCCTAATAGGGGCCTATTTCTACTT TTATTTTGTATTTCTGGTCTGTGAAAACAATTTAATAAAGGGAAATGACTTTGGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5005209 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5005209 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12329 same embryo
	EMAGE:12326 same embryo
	EMAGE:12327 same embryo
	EMAGE:12325 same embryo
	EurExpress:euxassay_007091 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source