EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12329

Aldh18a1 aldehyde dehydrogenase 18 family, member A1 ( MGI:1888908)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007071. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007071_01	euxassay_007071_02	euxassay_007071_03	euxassay_007071_04

euxassay_007071_05	euxassay_007071_06	euxassay_007071_07	euxassay_007071_08	euxassay_007071_09

euxassay_007071_10	euxassay_007071_11	euxassay_007071_12	euxassay_007071_13	euxassay_007071_14

euxassay_007071_15	euxassay_007071_16	euxassay_007071_17	euxassay_007071_18	euxassay_007071_19

euxassay_007071_20	euxassay_007071_21	euxassay_007071_22	euxassay_007071_23	euxassay_007071_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12329_wholemount_strong.wlz

12329_wholemount_moderate.wlz

12329_wholemount_weak.wlz

12329_wholemount_possible.wlz

12329_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12329_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4644
Entity Detected:	Aldh18a1, aldehyde dehydrogenase 18 family, member A1 ( MGI:1888908)
Sequence:	sense strand is shown >T4644 CCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGGGCGGTCGCCGCGAAGAGTGGTCCAGGGCTGGG GTCGGCGGCCAGCAGGTACTCCTGAGCACTAACACATACACACAAGGATTTGGACAATAGCCACCAGTCT TAGCAGAACAGGTGTTTGGATTTGTGACAACATCTCAACATGCTGAGACACATGCACCGCTCTGGGGTCC AGCCCTTCAGGCAACGTCTTCTACCATGGGTCCAGTCCATAGCTGTGCCCAGATCAAATCGTGTCCAGCC CTCAGCTATTAGACATGTTCGTTCTTGGAGCAACATCCCCTTTATCACGGTACCCCTCAGTCGTGCCCAC GGCAAGCCCTTTGCCCACCGAAGTGAGCTGAAGCACGCCAAGAGAATTGTAGTGAAACTCGGCAGTGCCG TGGTGACCAGAGGAGATGAGTGTGGCCTGGCACTGGGACGCCTGGCATCTATTGTTGAACAGGTCTCGGT GCTGCAGAATCAGGGCCGAGAGATGATGCTGGTGACCAGTGGAGCCGTCGCCTTCGGCAAACAGCGCCTG CGCCATGAGATCCTTCTGTCTCAGAGCGTGCGGCAAGCCCTGCACTCAGGACAGAACCATCTGAAGGAGA TGGCAATTCCGGTCTTAGAGGCCCGAGCCTGTGCAGCTGCTGGACAGAGTGGACTGATGGCCTTGTACGA GGCCATGTTTACACAGTACAGCATCTGTGCTGCCCAGATCTTGGTGACCAACCTGGATTTCCACGACGAG CAGAAGCGCAGAAATCTCAACGGCACCCTCCACGAGCTGCTCCGCATGAACATCGTCCCCATCGTCAACA CAAACGATGCCGTCGTCCCTCCAGCCGAGCCCAACAGTGATCTTCAGGGGGTAAATGTGATTAGTGTTAA GGATAATGATAGCCTGGCTGCCCGGCTGGCCGTGGAGATGAAAACCGACCTCTTGATTGTCCTCTCGGAC GTAGAAGGCCTCTTTGACAGCCCCCCAGGCTCAGATGATGCAAAGCTCATTGATATATTCTATCCGGGAG ATCAGCAGTCTGTGACATTTGGAACCAAGTCTAGAGTGGGCCTAGGGGGCATGGAAGCCAAGGTGAAAGC CGCCCTCTGGGCTTTGCAAGGCGGGACTTCTGTCGTCATTGCCAACGGGACCCACCCAAAGGTGTCTGGG CACGTCATCACAGACATCGTGGAGGGAAAGAAAGTTGGCACCTTCTTTTCAGAAGTGAAGCCTGCTGGTC CTACAGTGGAGCAGCAGGGGGAGATGGCTCGATCTGGTGGGAGAATGCTGGCTACCTTAGAGCCTGAACA GAGAGCTGAAATTATCAATCATCTGGCTGACCTGCTGACGGACCAGCGGGAAGAGATCCTGTTAGCCAAC AAAAAAGATTTGGAGGAGGCAGAGGGAAGACTTGCCAGCCCCCTGCTGAAGCGCCTTAGCCTCTCCACGT CCAAACTGAACAGCCTGGCCATCGGGCTGCGGCAGATCGCAGCCTCCTCACAAGAGAGTGTAGGCCGCGT CCTGCGCCGGACTCGGATTGCCAAAAACCTGGAGTTAGAACAAGTGACTGTCCCAATAGGTGTTTTACTG GTGATCTTTGAGTCTCGCCCTGACTGTCTACCCCAGGTGGCAGCCTTGGCTATTGCCAGTGGCAATGGCT TGTTGCTCAAAGGTGGGAAGGAGGCCGCACACAGCAACCGCATTCTTCACCTCCTGACCCAGGAGGCCCT CTCCATCCACGGAGTCAAGGAGGCCATACAGCTGGTAAACACCAGAGAAGAGGTTGAGGATCTCTGCCGC CTTGACAAAATAATAGATCTGATCATTCCCCGAGGCTCCTCCCAGCTGGTCAGAGACATCCAGAAAGCTG CCAAAGGGATCCCAGTGATGGGCCACAGCGAAGGCATCTGCCACATGTACGTGGATTCCGAAGCCAGCGT GGACAAGGTCACCCGACTCGTCAGAGACTCTAAATGTGAATACCCAGCCGCCTGTAATGCCCTGGAGACG TTGCTTATCCACCGAGATCTGCTGAGAACACCCTTATTTGACCAGATCATTGACATGCTCCGAGTGGAAC AGGTAAAAATTCACGCAGGCCCCAAGTTTGCCTCCTACCTGACCTTCAGCCCCTCAGAAGTGAAGTCACT CCGGACGGAGTACGGGGACCTGGAAGTGTGCATTGAAGTGGTGGACAGCGTCCAGGAAGCCATTGATCAC ATCCATAAGTACGGCAGCTCTCACACAGATGTCATCGTCACCGAGAACGAGAAAACAGCGGAGTTCTTCC TCCAGCATGTGGACAGCGCCTGTGTCTTCTGGAACGCCAGTACTCGCTTCTCTGATGGCTACCGCTTTGG ACTGGGAGCTGAAGTTGGAATCAGCACATCTCGAATCCACGCCCGGGGACCAGTAGGACTTGAGGGATTG CTAACTACAAAGTGGTTGCTTCGAGGGCAAGACCACGTGGTCTCGGACTTCTCCGAGCACGGAAGCCTCA AGTATCTTCACGAGAATCTCCCTGTTCCCCAGAGAAATTTCAGCTGAGAGGTGCCAGGCAGTCGGGACCG TTGTGCGCCGTCTGGCACATGGAGGCTACTCCAGGATCTCCAGAGAGAACCTGATCTCGTCCCTCACTTC TTGGAAGGGTCTGTCTTCTGGACAGTTCACCTATGCTTCTGGCTCTCCTCAGCGTTTCCAACCTTAGCTA ATGTTCACACTCCATTTTGGCCTTCTTGGGTGTGTCTGTCTCCGTAACTGGGGTTGAATCTAGGGCCTTG CACATGGTAGGCAAATGCTCCACTGCCAGGCTGTCTCCCTGGCCCTGCAGTCCAGTCCTAGGAAGAGAAT CGCTGTTGGAGACAGAGAGGCTTGGCTTTTCCCAGTCTGTTCACATCAGAGGTAGACCCTAAGGGACGCA TCTACCATCAGAGACAATGGAAAGTCCTGGCTGCCGGAACGCCTCAGTGAGGTAAAGTCTCTTGGCGTTT TGACTGGGACCAGCCCAGCCATTCTCAGCCTCTCGACATGTAATTTCATTTCTTTTACGAAAGCTCGTCT TAAGGAGGGCTAACTCCTTAAAACCTTTAACATTTTCATTGGAAACGGTGACTGAGACAGACTGACTGTA TCCACTGGGGAGATGACCTTGGTGTGTCTGTGAAGTTATGGGCGCACATCCTAGACATCTGCAGTCTGTC TCCGGCCTGGGGACACATGGTACCTTCAAAGTAGTTGGAATCTGAGCATCACGCTGGAGGGATGAATTTC AGGATGTTCCTACAAGTGGGAGGAAGATCTACAATCATGCTTGACCTTTTTATACAAATTATTTTGACAT CCTATTCTCGACCTCCAGGACAAAGACTAGAGATTATTTTAATACTTTAGATTCTCCCCCCCCCCCTTTT TGAGAAATAAAATTCTTATAAAAGCCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5009115 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5009115 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12326 same embryo
	EMAGE:12328 same embryo
	EMAGE:12327 same embryo
	EMAGE:12325 same embryo
	EurExpress:euxassay_007071 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source