EMAGE mouse gene expression spatial database

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EMAGE:12330

Zfp655 zinc finger protein 655 ( MGI:1919861)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007173. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007173_01	euxassay_007173_02	euxassay_007173_03	euxassay_007173_04

euxassay_007173_05	euxassay_007173_06	euxassay_007173_07	euxassay_007173_08	euxassay_007173_09

euxassay_007173_10	euxassay_007173_11	euxassay_007173_12	euxassay_007173_13	euxassay_007173_14

euxassay_007173_15	euxassay_007173_16	euxassay_007173_17	euxassay_007173_18	euxassay_007173_19

euxassay_007173_20	euxassay_007173_21	euxassay_007173_22	euxassay_007173_23	euxassay_007173_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12330_wholemount_strong.wlz

12330_wholemount_moderate.wlz

12330_wholemount_weak.wlz

12330_wholemount_possible.wlz

12330_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12330_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 14 15 moderate expression: see section 08 09 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9480
Entity Detected:	Zfp655, zinc finger protein 655 ( MGI:1919861)
Sequence:	sense strand is shown >T9480 CGGACAGGCTGGTGCAGCTGAGCGAGGAGCGCCTCCGTGACGGGACAGTCTCCAGGCCCGCCTCTCCACC TGGTGATGGTCATTGTCCCCTGAACAGGGATGGAGGAGGTAACATCCCAAGAAGCCGCAGAGTCACCCAG GGGCCACTTCCAGCCTTTGGAGAACCAGTCTGAATGTCTTTCCCCAGAGCTTCGGTTTCTGCAGGACACG GACATGGAGCAGGGATTCAGTGGGGGATTTCCAATTTCGAAGCCTGATGGAATCTCTGAGCGGGAGCAAG ATCTACAAGTCTTTGATCTGGAGTCTAAGAATAGAGAAGTCATAAGAGGTGACTGTTCAGATGGAGAGAC CAGAGAAGAAAACAAGCTGTTGATTCCTAAGCGGAAAATTTCAGAAGAAGTACATTCATACAAAGTGAGA GTAGGAAAATTCAAACAAGATATTGCTCAAGTTCCTGAAACCAGAGAGGTTTATAAGTCTGAGGACAGAT TAGAAAGACTCCAGGAGATTCTAAGAAAATTTCTCTTTCTGGAGAGAGAGTTTAGACAAATAACAATCAG CAAGAAGACTTTCAGCAGTGAGAAGAACACTGAACCTGAAAAAAGCTTCAGTCTAGACTCCACACTTGAT ACAGATCAGAGAGTTCTTAGAATACAGACCACTGATGACAGTAAATATGACATGAACTTCAACCAGAACC CAGCAGTTGGTGAACAAGAACCAATAAATCTAACAGAGAATTTTCAGAGTACTGACTATAAGGAAAGCTT AATGGATCTGTCCCACCTTAATAAGTGGGAGAGCATGCCTACCACCGACAGATCCTATAAATGTGATACT TGTGGGAAAACCTTCCATCAGGCCTCAGCTCTTACTAGACATCAGAGAATTCACACAAGAGAGAAACCCT ACAAATGTAAGGAATGTGAGAAATCTTTCAGTCAGAGCTCAAGTCTTAGTCGACACAAAAGAATACACAC CAGAGAGAAATCCTACAAATGTGAAGCGTCTGATAAGTCCTGTGATGCACCTGATAAGTCTTGTAGCCAG AGCTCAGATGTGCTTCAACATAAGAAGGGTCATGCCAAAGCCAAGTCTTACAAGTGTGGAACCTGTGAAA GAGTCTTCAGCCGAAGTGTGCATCTTACTCAGCATCAGAGAACACATAAGGACATGTCCTGTAAATGTAC CGTGTGTGGCAGTGACTTCTGCCACACCTCATACTTAGTTGAACATCAGAGACTACATCATCAAGAGAAG TCCTATGAATATGATGAATGTGGGCTGGCCTATGTTAAACAACAAGGAATTCGTTTCCAAGAAAAGCCCT ACTCATGTAACGAATGTGGGAAAGACTTTAGATTGAATTCCCATCTTATTCAGCATCAAAGAATTCACAC AGGAGAGAAACTGCATGAATGTAATGAGTGTGGGAAATCTTTCAGCCAAACCTCATGCCTTATTCAGCAT CACAAAATGCACAGGAAAGAGAAATCATACGAATACAACAATTATGAGGAAAGTTTCAGTCACAGTTCTG ATCTTACTCTGCAACAAGAAGTTCCCATTAGAGAGAGAGTCTTTGATTGTGATGCATGGGAAGAGAACTT CAGTCAGAGAGCACACCTCATCCAACATGAAAGGGTCCATACCAAAGAGAAACCTTATGAATGCAGTGAA CTTGGGGAGACATTTAGTCAAGTTCAGGCCTCATTCAATATGTGAGAACTGAGACCAGGGAAAGTCACAT ATGTACTGAATGCTATGATGTCTTCAGTCATAGCTTATTCCGTGGTCGGCATCAGAGAATTGATAACCAG AGAGAAACCCGCTGAATGTGATGAACATTGGGAAGGTTTTTTATTTTTTCTTATTTTTGGTGTTAAATTC TTCATTGGTTCCTGTAAATCAGTGCTAGAATATATAGAATATGATACATTCTTCAGATTATTTTCATACC TTAGTCACAGTTGGGGAATTTGTACTGGAGTAAAATTCCACTGTCAAATGTGAAAAAGCCATCAGTCAAC TACCTTGTTGGTTCAGTGTCAAATTTGTGCCATGCATAAAGCCTGTGAAGACATAGGTGTGTATGTGAGG AAACTCAAGTCATACCCCAGTGCTTGATCACATCGAGGAAACTATACCCAGAAATAGTCTATACCCAAGA AATTATTTGGATATAGTAAATGTGGCAGATCTGCAGGAGTCCATCTGTCATCAGAATGTACCAGATGTGA GTCTGATTGTTATGCAAATGCTAAGATCAAAACATCTGCACCAGATGAGACTGATATAATGCAAACGGTG AAATTTAACCACTCTATTTTCCAGGCCTTATCCAGTATCAAAGTATTAGAAAATGCTTACTTCTCTGTGT AATTATTACTATTTAGTCTCAGTCTTTTTTACTGCTGAATAACTGAATCCAGAAGATAATTGGGAAACGA AGTCATTTTCTCACAGTATCCTTTAATAAGTATTCTAAGAAGGTACAAGTATTTTATTATAGAACTCATT TGAGTGAATTTATTTTAATTTGGCCGATACACATTAAAACATTTTTCTTCTTTGAAATGGATTCTTATTC ACAGTTTACCTAGAATACTGTTAATGTGCCAGTGTTGACATAGCTTCTGGAGAGTAGCTCAATGAGCCTT CTCTGTTCTGGCCAGAGTACTCTCCAAAGGAGACTCAGGCCTATTCTTTTCTGAGGACCCTACAGACTAT TGTTTCTATAGCCACACCCTTGGCTTCCTGTTGATTTCTGCGTTCTCTTAGGCATCCTTAAATGATGTTC TGGTGTTAGCTTCTCACCAGCTGATAAAGAGTTACTTCCACATCAGCCATCCATACGTTTGGAGGATTCA GTCTTCCTGAGTTTGTGGCAGGCACCCAAACATTTGCAAGTGATGTCCAAGTTTTTAGCTGCCTTGTTTG CATCCACAGCCCTCTAATGAGATGACAAGGACATCAGGGAGCCAGTAAGACTCCTGTTGGCCTAGCTACA TTCATTCAGTGCCTACTTTGCATGTATGTGTATGTTTAGAATTCTTTTATATGCCTATATTGAAGGCCAA CAAAATGCTCAGGTCTGCTTTTGATAGGGAAACAAAGAACACACAAATCAGAATAAGGATATACAGCCTT CCAATAAAGATATTGCTGTTAGTGATGTATCTAGACAGTATTTCAAGAGAGTGGCAGGTCCGTTCCTGGT AAAACCTTAATCATTAGGATTGCTTCTTTATCTGTTCCCTCCTCCACTCCCTTCATCAAGCCAGGGTAGT ATTTTGAAGCATAAAGCTTTGTGCATAATTATTTGTGCATTGCCATACTTTTGTGTTGGGATGCAGTCTT TGTTGCCCAGGCTGGTCTTGAATTCCTGGGTTCACACAATTTCCTGCCTCCCAAATAGCTGGGATTATAG GCATGCGCTCCTGTGCCTGGATTTTATTTTTGTATTTTAATAGAATCTGCAGGCCATATAAAGGATCATG GCTCTTTTGATTTACAGAAGTGGTCACAGGTGTTGAGTCCAGGGTCCCTGCCCGATGCCACAGAGTTCAT GATTTTTTTACATGATGTGAAGCATTTTCATCTTAAAATTTGTAACATCTTGTGAATAAGAAAAGTGTTA TTTCAAATTCTAGTTGTCAATCAAGCAGTTGAGGCTTTCATAGTTCAGATGTCATAATGTTCACTAGGGA TCCTCAACCAGTATATAAAATATGCTGCTCACTGTAAATCTTGTGGCTGACCTCTAGGGTAGTCCCACCA CTGTATTTTACTTCTTTGCCATCAGCAAGCGTAGTGTCTCATCAGGACAAGAAAATCAACATATATCAAA TTGATCTAAATGTAAAAGCAAATGAAAAATGCATGGTTTTTTGTCCCTGTCGAACATGTATATTCTTACT TATAGAGACCAATAGTCACTTCCAGTGACTGAGGCAGAGGATTTGATTCATTCCTAAGTAGCAGAATCTG TAAAAACCCCACGTATGCATTTGGGTTTGGAACGTGCTTTTGTACTTCTACTTGAATAAAGGTGTGTTCA GTGCTCGTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30357186 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30357186 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12331 same embryo
	EurExpress:euxassay_007173 same experiment
	MGI:4829329 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source