EMAGE mouse gene expression spatial database

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EMAGE:12331

Tppp tubulin polymerization promoting protein ( MGI:1920198)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007135. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007135_01	euxassay_007135_02	euxassay_007135_03	euxassay_007135_04

euxassay_007135_05	euxassay_007135_06	euxassay_007135_07	euxassay_007135_08	euxassay_007135_09

euxassay_007135_10	euxassay_007135_11	euxassay_007135_12	euxassay_007135_13	euxassay_007135_14

euxassay_007135_15	euxassay_007135_16	euxassay_007135_17	euxassay_007135_18	euxassay_007135_19

euxassay_007135_20	euxassay_007135_21	euxassay_007135_22	euxassay_007135_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12331_wholemount_strong.wlz

12331_wholemount_moderate.wlz

12331_wholemount_weak.wlz

12331_wholemount_possible.wlz

12331_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12331_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 09 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 17 18

trigeminal v nerve

moderate

regional

moderate expression: see section 09 16

spinal cord

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 15 16 17 18

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17 moderate expression: see section 06 07 18

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 14

heart ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9267
Entity Detected:	Tppp, tubulin polymerization promoting protein ( MGI:1920198)
Sequence:	sense strand is shown >T9267 CGCTGAGGCGCAGCAGTGGACGCGTTTTGGAGCAACATGGCTGACAGCAAGGCCAAGCCTGCCAAAGCTG CCAACAAGACACCACCCAAGTCCCCAGGAGACCCAGCCAGGGCTGCTAAGAGGTTGTCACTGGAGTCAGA GGGTGCCAATGAGGGTGCAACTGCAGCTCCTGAGCTCAGTGCCCTGGAGGAGGCCTTCCGGCGGTTTGCA GTACATGGGGACACCAGGGCAACAGGAAAGGAGATGCATGGCAAGAACTGGTCAAAACTGTGCAAGGACT GCCACGTGATCGATGGGAAGAATGTGACCGTCACTGACGTGGACATTGTCTTCAGCAAGATCAAAGGAAA GTCCTGCAGGACCATCACCTTTGAGCAGTTCCAGGAGGCACTGGAGGAGCTGGCCAAGAAGCGGTTCAAG GATAAGAGCAGCGAGGAGGCTGTACGTGAGGTGCACAGGCTCATTGAGGGACGAGCACCAGTCATCTCTG GCGTCACGAAAGCTGTGTCCTCACCCACGGTGTCACGGCTCACAGACACAAGCAAGTTCACAGGTTCCCA CAAGGAGCGTTTCGACCAATCAGGAAAGGGCAAGGGCAAAGCTGGCCGTGTGGACCTGGTGGATGAGTCA GGCTATGTGCCTGGTTACAAGCATGCAGGCACCTATGACCAGAAGGTGCAGGGGGGCAAGTAGCCTCATA GGCTGACGCTCTGGACACTATAGGTGGCAGGCACAGGACTTACACCCATTGCACTTCATTACATTCCTAT GTTTAACTGGGCAGAACTCAGAATGCACCTCCACTAGGCGATGGTGGGGGCAATGCCCGATCCTCCTCCT GCTGGGTGCTTTGACACTTCTCCCTCAGACCCCAGGTTTGGGCCGTGATTCACTGTCCTGTCCTAACATA CCTGCCCTGTCCACAGCTGGAAAGTGGACTGCTATTTCCAACTGTCTCCCAGGATCATACCATACTGGCC ACTTGATTTTCAGGTAAAAGGGGGTGGTTTGGGTAAGGAGAAGCAGAGCAGATGCCAGAACTCACGCTGA CCTTATCTAGGCACTTCTAGGTCAGCAGGAGCTAAGAAACTAACCAACTGCATAGAGTAATCCTAAACAG GCCCTTTCATCTTTCTTGACCACAGCACATGGCTGAGGCTACTTGGTGGGTTAGCACACATGTGTGTGAA TACACATATGCACACATGCATATTAACCAACCGAGTCTGTGCTGTGAGAGGCCAAGAGAAAATGGAAGTT CCCCAGCGACCAGGAGAGTGACTTGGAGTATGAAGCTGGAAGTTTCTTCAGTGTTGTTTTAAGAAATGAC AAGACTATATGCTTGGTCTCAAGAGTGATCCAGGGAGATCCAGAACATCAAGCTGCCATGTCCCATCACC AGGTTGGGACGCACATCATGCTGAGACACTTTGTCCAGACCAGGCCTGGACAGTTGTTCCACCAGTGTGA GATGGAAGCTTTTACCAGGGCAAGTTATACTGTGTTTTGCTCAAAATTATGTGTCTTCAGCTCTATGGAG CCTTCTCTGGGTTCAGTTTTCTTAACTTTTCTCACAAGTCTAAGTGTAGGGCACTGGAAACCAGAACTAG GCCCAAGAAAGATTTGCAGCATCTGCTAAGGTTTCTTTTTCTGCCACAGGAGAGAAGTCAGGACTCAGCC CATCCATGTGTGAGGATGGCACTTAGCTAGGCCCAAAGGCATGCACCATCCTCATAAGAGCCCCACAGCA ATTGTTCCTTTGCATTAACACAGCCCTAGCCTTGCATGCAACTTGGTGGACTGTGTGGCCTTGTTTCTAG AAAACACACAGTGGCCTCAGGATATGCTTCCTGGAGCAGATGGGTGTTGAGGGTGGGACATTTTACATGG CTTGGACAGCATAGGTAGACAGCAAGGCTATCTTCCCCACTGCCATGGCACATAGTCACATTATCCACCT CTCTGGGGTTCAGAGAGCATGGCTTCACTGCAGCACCAGAGAAGACTACCTACTAGCCTAAGAGAGAAAA GATGGGCCTTAATACCAGACCTCCCAGACAGCAGGCTCTCTGAAAGTTCAAAGTTAACAAGCTCAAGGAA AATGGGGATGGTTGGCTTCCTCCCAAGGTTTTTTGAGTTTGCTGTGCCTGGCAAGTGGTGGAGCCTCCTT TCTGGAATCTTACCTCACTGGTCTGGGGAAACAAAATGACCCTTGCACTTCTTGCACCCTCACCCAGAAC CCCTAGTCCCCATGTGGCTGGCAGTCTCCTGCTCTGAGGATGCTCTTTTCTACTGCCACCATCACAGCCA AGTAAAATGGAACACACACACAAATCCATCAGGAAGTGACTGCATGTGGGCCAGGGCAGAGGGGAAGGCT AGTAAGGCATCAGAGGGTCCCCACAACATGTAGTGGGTCAGATGTTTCCCTGACCCCCAAAATAGTACGA AAACCCTGGACAAAGCCTTCTGTTCAAAGGGCTAAGATGCCAGAATGATGTCCAGATGAAGATTCAAGCC AAGTCAAGGGTTAACAGCCTAGGAACACCTAGGAAACAACATAAGTGTCCACCTTCACGTGACCGCCCAG CGTCATGGGGAAGCTTCATTATAAACTGGTCACCTTACCTATGTGCTCAGGACTGAACAGAAAAGCTCAG AGTGGCAAGCCCGCATAGGTCCTGGGCAGTAGAGGACCAGAACTGCCATCAGAGTGCCCAAAGGTATGAT CCTCAGCAGGAAAGCATTCCAGGAAAGCAACTGAGAAAGCATTTCTAATGGCTCTTACACAGCTTGGACT TTGTAAATGAAAATTGGGTTACTCTGCCTTGCTGGTCCTTACTGTCCAAGAGCAGAACCTGAGCACCCTT GGGTCCTGAGGGATAGTCCTAGGCCTGCCCATATAGTTCCCCAGCAACACCCAGAACTATGTCAGACAAG TGGTCTTCATTGTGTTCCCCCTGCCCCCCAGAGTTTCCTTACACAGCACCTTTCTTTAGTCAGTGTTAAG TTTAACTTTGAAAATAAACGCTCCCTCTAGATTATATGTCATCTAGCGAGGGAAGGGTAGTAACAATTAG ATCACGGAGACTTGGTGTCTTTGTACCGTCAGTTTAAAATGTCTATGGAATTCATTGTGACATCTGTGTA CCCTTTCGACATAGAAACTGATTTGAATCAAACTTTGCTGCACAAAACAGATGTGTATTTTCCTCAGTTT GATTTGAACACACAGGTAGAGTGTTTGTCTCTGCTGCATGTTTTATGCAATACCAATTAGCTATTACTAA TTATGGATAAATAGAATTCAGTGTCTGTGAATAAAAACAAACAAACAAAAACTGCACAGCATGCAAACCA AGACCCAGGGCCATGTGCTATTAGGTGACAGGCACACGAGACAGGGATAGGTTCCCATAGAGGGTGACAC TCAGGAGGTGACATTTTCATTTCTAGAGGGGACACCCAGGAGACAGAGACATACTTTGTTGTGAGCTGAC CCAGGAAACAAAGGATACATTCTATCAGGGGAAGACACTCAAGAGACAGAGACAGGCTTTCTTTGAGTTA TGACAGCAAAGAATAAGGACAGGTTCTGTCAGGGGCGACACTCAGGGACATGGATTGGAGTGACAGAGTC ATATTTTGTTGCACGTGACACTCGAAGATATGTTTTGTCAGAGTGAAACCTAGGAACATGGTCTGTAGAG GGTGACATACAGGAATACATTCTGTTGGAGGTGACATTCAGGAGAAAGGGAAAAATTGCAGGGAAGAGCC ATGCAGATGGTGTCTTAAGTGTCAGGGAACGATGGTAGGTAGGTTGGAGGCTGTCTGCAAACACTGACTC TAGTAGAACCCCTATTTATTTCTATTTAATGTAACGAACAACTAGTTAGTGGAAATGCTTTACCAAAATC AGAGTTCAAATTACGTATTTAAATATTGTTGAAGATCTATATTTATACTGGAGTATTTTTACACCTTTCA GTATCCTATCTTATATTGGGGCATAGGGGCATTATGACACATGGAGATAGTTCTGAACTGGGCTGCACAA CAGTTCTGCCACCTCCCTGTAGTCTGTCCCAGGGAGTTGACAGGTGCCTGCCCTGAGCCAAGACCCACTC GCAGAGGCCCTGGGCTGCCTCAGCTGCCTGTTTTAATGAATAGTTTTACTGGCACACAGCTGTACCCATG TGTTTACATACTGTTCTGTAGCTGCTTTGGTATTAGAACTAGCTGGGAGGATTGAGACAGACGCCATGGC ACAGACACATATGGTATTTGCCACCTGTTCTTTTTCAGGGAACACTGCCTGTCTGTCTGCTTGGTTCTCT CTCTGTAGACTTGTGTACTACCTGGCTACAACATGGGCACATGGGGGAAGCCCTGCCAATTAAGAATGGC AAGAGTGACAATTTAGCTGTACTGTGAAGACATCAGTAGACAGAATATCCTTCCAACACTGAGCCAAACT GCTCTTCTTCACCAACATACTACTGACCATTTTACAGTTCAGGGGTGTGGCCATCTTGGGACGCTTAAAT TATCTCTTCCTTTAGATTTAACCAAGCCAAAAAACAAAGGAAGCCTGTACAACAACTTAAACTTGAAAGG AATAATATGCTACTACTACTAGTTTGTACTAAGTTTTTTTTTTTCAAGAAAGGGAGTTAAGTTTATATAT ATGTGATATATATTTTTACACTATTTTATTAATGTTAGGGATATGGAGTTTATCACTTTATCAGTGTGAT GGGTAATGTTGATGTCATGGGTGTTCTGACTCAGAAAGACACTTATACTGACATCTAAGCCACACTTGCT CAGACTGTCTGTCAGGTCAGGATGGCCCTGCTGTCCCTCCTATAGAGCGCAAGGCGTCTAGTCACCTAGT TGGACATATGGCCTGGCTCATATTGCTTTAGTGAAACAGCTTGTGTCTTTTATTTCGTTTATTGTGTTTG CTGTTATTAACATTGTGTGTCAAGTTGGTAAAGTGATGAATAAAGGTTTTAAAATATGGAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6837919 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6837919 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12330 same embryo
	EurExpress:euxassay_007135 same experiment
	MGI:4828881 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source