EMAGE mouse gene expression spatial database

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EMAGE:12335

1500010J02Rik RIKEN cDNA 1500010J02 gene ( MGI:1916214)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008529. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008529_01	euxassay_008529_02	euxassay_008529_03	euxassay_008529_04

euxassay_008529_05	euxassay_008529_06	euxassay_008529_07	euxassay_008529_08	euxassay_008529_09

euxassay_008529_10	euxassay_008529_11	euxassay_008529_12	euxassay_008529_13	euxassay_008529_14

euxassay_008529_15	euxassay_008529_16	euxassay_008529_17	euxassay_008529_18	euxassay_008529_19

euxassay_008529_20	euxassay_008529_21	euxassay_008529_22	euxassay_008529_23	euxassay_008529_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12335_wholemount_strong.wlz

12335_wholemount_moderate.wlz

12335_wholemount_weak.wlz

12335_wholemount_possible.wlz

12335_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12335_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9318
Entity Detected:	1500010J02Rik, RIKEN cDNA 1500010J02 gene ( MGI:1916214)
Sequence:	sense strand is shown >T9318 GTATTTCATCATGACACTTGGTGAATTAGCCCAAGCTGGCAGCCAGGTGTCCATCATTGTACAGATCCCT GCTCAGATGGTGTGGCACCGAGTCCTCCGGCCTGGCAGGGCCTATGTACTGACAAAATTGCAAGTAACCA AGACCCGGATTCACCTTTCCTGTATTTGGACAACCATTCCATCTTCAACTCTGAAGCCTCTGAGACCAGG TTATGTGCAAGAGCTGGAGCTGGACTTAGAGTTCTCGAAAGCTGACCTTAAACCACCACCCCAGCCCACC AGCTCCAAAGACAGCAGAGGGCAAGAAGGTCTTGTCCGGGCTTCCAAAGTCTTGCATTATTTGGGAACAG TCACTGCTGTGTTGCATGAGTCTGCCGGCCTCTACATTCTAGATGGGCAGCTGATTCTCTGCCTCGCATA CCAGAAGATCCATGGCCTCAGGCGGGTTATTCGACCAGGGGTCTGTCTGGAGCTCCGAGATGTTCATCTC CTCCAGGCAGTGGGTGGAGCAACAACAAAACCTGTCCTGGCGCTCTGTCTCCATGGCACTGTTCGGCTTC AAGGCTTTTCTTGTCTTAAGCCTCTGACTTTGCCGTCCTCCAAAGTTTATGGTGCCTCCTTGTATGAGCA ACTCGTGTGGAAATGTCAGTTAGGACTTCCCTTATACTTATGGGCTGCCAAGACCCTGGAGGATCTCATC TACAAGCTGTGTCCCCATGTGTTGAGATGTCACCAGTTCCTGAAGCAACCCTCTCCTGGGAAGCCCAGCT TGGGCCTGCAGCTCCTGGCTCCCTCCTGGGATGTTCTGATTCCACCTGGCAGCCCCATGCGGCATGCGTA CAGCGAGATCCTGGAAGAACCACACAACTGCCCCCTTCAGAAATACACTCCACTGCAGACTCCCTACTCT TTCCCTACTATGCTCGCCCTGGCAGAGGAAGGGCAGCACAGGGCTTGGGCTACTTTTGACCCAAAGGCCA TGCTGCCTCTCCCAGAAGCCAGTCACCTGACCAGCTGCCAACTGAATCGCCACCTAGCCTGGTCCTGGGT CTGTCTGCCATCCTGTGTGTTCCAGCCAGCCCAGGTTTTACTTGGGGTCCTGGTGGCTTCATCTCGTAAA GGTTGTCTGGAGCTTCGGGATCTAAGAGGTTCACTGCCTTGTATCCCCTTGACTGAGAGTTCACAGCCCC TCATTGACCCGAATCTCGTAGGCTGCCTGGTGCGGGTAGAGAAGTTCCAGTTGGTAGTAGAGAGAGAAGT CAGGAGCAGCTTCCCTTCCTGGGAGGAGATGGGCATGGCACGCTTCATCCAGAAGAAGCAGGCCAGAGTC TACGTCCAGTTCTACCTGGCTGATGCCCTGATCCTGCCCGTGCCCAGACCCACCTTTGGTTCAGAACCAT CTCAGACAGCCTCCTCCTGCCCAGAGGGACCCCACCTGGGACAGAGCCGGCTGTTCTTACTGTCCCACAA GGAGGCCCTCATGAAACGTAATTTTTGTCTCCTTCCGGGAGACAGTTCACAACCTGCAAAGCCCACCTTG AGTTTCCACGTGTCAGGAACTTGGCTTTGTGGCACACAGAGGAAGGAAGGTTCTGGATGGAGCCCACCCG AGTCTCTGGCCGTTGAGAGCAAGGATCAGAAGGTCTTCCTCATCTTTCTTGGCTCTTCAGTTCGATGGTT TCCGTTCTTGTACCCAAACCAAGTCTACCGACTCGTGGCTTCCGGCCCCTCACAGACACCAGTGTTCGAG ACAGAGGGCTCAGCTGGCACCTCTCGACGTCCTCTGGAACTGGCTGACTGTGGATCTTGCCTCACTGTCC AAGAGGAGTGGACCCTGGAGCTTGGGAGCTCTCAGGACATACCTAATGTACTGGAGGTCCCTAGAACACT GCCCGAGTCCTCACTGGCCCAGCTGCTCGGTGACAACTCCCCCGATTCCTTGGTATCTTTCTCTGCTGAG ATTTTGTCTCGGATACTGTGTGAACCACCTCTGGCCCTTCGCCGGATGAAACCTGGGAATGCTGGGGCTA TTAAAACAGGTGTGAAGCTAACAGTAGCTCTAGAAATGGATGACTGTGAATATCCCCCTCACCTGGACAT ATATATCGAAGATCCACAGTTGCCTCCCCAAATAGGACTCCTTCCAGGAGCCCGAGTCCACTTTAGCCAG CTGGAGAAAAGGATTTCCAGATCCAACATTGTTTACTGTTGTTTCCGGTCATCCACTTCTGTGCAGGTCC TGAGCTTCCCCCCAGAGACCAAAGCCAGTGCTCCTCTGCCCCACATCTATCTGGCTGAACTTCTCCAAGG TGACCGGCCTCCCTTCCAGGCCACTACCTCTTGCCATATTGTGTATGTCCTGAGCCTTCAGATCCTGTGG GTGTGTGCTCATTGCACCAGCATCTGCCCCCAGGGTAAGTGTAGTCGCCGGGACCCCAGTTGTCCATCTC AGAGGGCTGTAAGCCAGGCCAACATCAGGCTCCTGGTAGAAGATGGCACCGCAGAAGCGACGGTGATCTG TAGGAATCATCTTGTGGCAAGGGCACTAGGGCTGAGTCCTAGTGAGTGGTCCTCCATCCTCGAGCATGCC AGGGGGCCGGGGAGAGTGGCCTTGCAGTTTACAGGGCTAGGAGGCCAAACAGAGTCTGCAAGCAAAACCC ATGAGCCCTTGACCCTGCTCCTCCGGACACTTTGTACCAGCCCCTTTGTCCTCCGACCCGTTAAGCTTTC TTTTGCACTTGAAAGGAGACCCACTGATATTTCCCCACGAGAACCGTCCCGGCTGCAGCAGTTCCAGTGT GGAGAGCTCCCTCTGCTGACCAGAGTGAATCCCAGGCTCCGACTGGTCTGCCTTTCTCTGCAGGAGCCGG AGCTCCCCAACCCTCCACAAGCATCTGCTGCTTCGTCTTGATGACACTGCCTAAGGTGGCCCCAGAGTTT CCAGGACCCCAGACACTGGAGTGAGGAACTCCTGCCCCACTGTGATTTCTAGTTATTCTGGTGTCAGCAT GCCTTGGAGACACTGAAAACGGCAGATCACAGCATGATAATGGTGTTTCCTCCAATTGGGTTAGAACACC TACACTCAGAGGTGACCCTCCCTGAGTGCTTGGGTTCTGCCTTGTGGAGAAAGCAGGACGGAGACCTTGC CCCTTATGATTGATTGTATTGTGATAGGCCCTTTGGGCTACAATAAACTTCTCTGAGGACTTTCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6847619 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6847619 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12332 same embryo
	EMAGE:12333 same embryo
	EMAGE:12336 same embryo
	EMAGE:12334 same embryo
	EurExpress:euxassay_008529 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source