EMAGE mouse gene expression spatial database

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EMAGE:12349

Pigk phosphatidylinositol glycan anchor biosynthesis, class K ( MGI:1913863)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008270. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008270_01	euxassay_008270_02	euxassay_008270_03	euxassay_008270_04

euxassay_008270_05	euxassay_008270_06	euxassay_008270_07	euxassay_008270_08	euxassay_008270_09

euxassay_008270_10	euxassay_008270_11	euxassay_008270_12	euxassay_008270_13	euxassay_008270_14

euxassay_008270_15	euxassay_008270_16	euxassay_008270_17	euxassay_008270_18	euxassay_008270_19

euxassay_008270_20	euxassay_008270_21	euxassay_008270_22	euxassay_008270_23	euxassay_008270_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12349_wholemount_strong.wlz

12349_wholemount_moderate.wlz

12349_wholemount_weak.wlz

12349_wholemount_possible.wlz

12349_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12349_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9170
Entity Detected:	Pigk, phosphatidylinositol glycan anchor biosynthesis, class K ( MGI:1913863)
Sequence:	sense strand is shown >T9170 GGGCGTGAGAAGCCGGCAAACATGGCGGCCCCCTGCTTCCTCACTCTGCGGGTGGCTACCCTGGCCGCCT TGGCGCTCTTGTCTCTCGGCAGCTCTGCCGCTGGACACATCGAGGATCAAGCCGAACAGTTCTTTAGAAG TGGACACACAAATAACTGGGCTGTTTTGGTGTGCACATCCCGATTCTGGTTTAACTACCGACATGTTGCA AATACTCTTTCTGTTTATAGAAGCGTCAAGAGGCTAGGTATCCCGGACAGTCACATTGTCCTGATGCTTG CTGATGACATGGCGTGCAATGCTCGGAACCCCAAGCCAGCCACAGTGTTCAGCCACAAGAACATGGAGCT CAATGTGTATGGAGACGATGTGGAAGTGGACTACAGAAGCTATGAGGTAACTGTGGAGAACTTTTTAAGG GTATTGACCGGGAGGGTTCCACCCAGTACCCCTCGCTCAAAGCGTCTTCTTTCCGACGACAGAAGCAATA TCCTCATTTATATGACAGGTCATGGAGGGAATGGTTTCTTGAAATTCCAAGATTCTGAAGAAATCACCAA CATAGAACTTGCAGATGCGTTTGAACAGATGTGGCAGAAGAGACGCTACAATGAGCTGCTGTTCATTATT GACACTTGCCAGGGCGCGTCCATGTACGAGCGGTTCTACTCTCCTAACATCATGGCCTTGGCTAGTAGCC AGGTGGGAGAGGATTCGCTGTCGCACCAGCCTGACCCTGCGATTGGAGTTCATCTTATGGATAGGTACAC GTTTTATGTCTTGGAATTTTTGGAAGAAATTAATCCAGCTAGCCAAACTAACATGAACGACCTTTTTCAA GTGTGTCCCAAAAGTCTCTGTGTGTCGACCCCTGGACATCGCACTGACCTTTTCCAGCGAGATCCAAAAA ATGTCCTGATCACCGATTTCTTCGGAAGTGTGCGCAAGGTGGAAATCACAACAGAGAAGATCAGTTTGCA GTGGGATTCACAAGTCGTGGACAGCAGTTCTAAAGAAGACGGCACGGCCGAGGAGCGCATGGGACCTCTC AAGTATGCTGAGCAGCTCCCGGTGGCTCAGATAATACACCAGAAGCCAAAGCCGAGAGACTGGCACCCTC CCGGAGGCTTCATCCTGGGGCTGTGGGCGCTCATCATCATGGTCTTCTTCAAGACCTATGGGATCAAGCA TATGAAGTTCATTTTCTAAGACTCAAGGATGTATGAGGAAGAATGAATGGAAGACTGCAGCCTTGGAGCA AAACTTAGGTTTTCATATGTTTTTAAATATGTTGCTTTTGTGTAAATTTGGGAAGAAAATTAGGAAAATT GAATATTAATGAACTTTTGACTTTAAAACATAATATTGTTAATGCACTTGTTTACTGGCCACAGAATATA TCTTTCATTGTGTTTGTGTGTTTGAGTTATAGAAAGCAGAGAGCAATGACATTGGAACTTTATGCTTAAT TCTTTTTACCCTCCTCTCATTGTAAAAGTGAGTAGTAAAGGTGTTAGTTCCCACTAAAGGAAATTACTTC CGTTCATAGGTTTGTGTGTGCTGTCTTGGTCTGTTTATCTATGGACATATGTTCCCAACCATACAGAATA TGCCATCTCTAATCTTTGGTGTTTACAATGCATTATTAACCTTTCTCCGCCCTCGGACATCCTTCAGGAT ATACTGTGAGCCTGACTTGGATGCACTTGCCTACAGTCCAGGCTGTTCAGGAGGCCGAGTCGATTTTTGA GCCCTGGGGGTGTTGAAGGTCAGCCTGGGCAACAAACAACAACCAACAGAAGAGAGAAACACCACGGAAT GCACCGCCCACTCTATGGTGGTCACGTGACAGCTCCTGAGGGACCCTTACCTGTTCCTAGAGTTCCCTTG GGCTCACTCTGGGATAGTAATGCCTCTGGAATTCTGCAGCTTTACTCGCACAGGAAGATGGAGACACAGC TTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCA CAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTAATTGCACAGGAAGATG GAAACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGC TTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCA CAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATG GAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGC TTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCA CAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATG GAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGC TTTACTCGCACAGGAAGATGGAGACACAGCTTTACTCGCACAGGAAGATGGAGACACAGCTTTAATCGCA CAGGAAGATGGAGACACAGCTTTGGTAGCTGTTTATCTAGGGAAGTCATGCCTCTGAGAATTTTTAGTGG TAACCCCTCAGAGAAACATGCCCTATTACTTTTTATTTTTAAGCATACTGTGAACTTCTAGAATTGATTG TATCTCTTCAGTTTTTGTCCTGAAAGAGACAGATTGCTGACAGCTGGATCTATGCTGTCCCTGAGGACAG CCCCATAAACTGGATGACATACATGGAGCACACACACTGAAGGCCCCGCCTTCATGTCTGATGGATCAGT AAGACACATCCAATACTGACTCTTCTAGGATGTCAGTCACCTCCACATCTTCCTGCGGTGAAAAGATGGC CAAGCTTGTGTTTTGTGACCATTCTCAGACACTCTTTTCTCTTTCAGACTTTGGCATCAGAGAGCCGTGC TGGGGTGGGCTGTGCCTATAGATTTACTATTCATGTCTGAGGAGAATCTTAGGTGTTTCATCCAAGAATC TATATGTTCATCTGTTTTCTTTACAAATACTGTTTTCTAATTGTAGGTAACAATTAAGAAGTCTTCTAAT CCCCTGGCAATAGGCAGATACACCTTGTTTGTGATATTGCTCTTTTGTCAGTCAGGGGCAGAGCACATGG CAGTCCTTGTTCATCCTAGCTTGTTGCCTGCCCTGGTGGCCAAAGTTGTGTCATGCCTAGGACACATAAA AATCAAATCATCAAATCAAATTGAGGCACCTTGGGAGGAAGACAGTAGACAGAGGCCTGTGGACCATGGA CCACGTGCCACCCACAGACCTGCTCTCTGATGTATGACACAGGTGGGCTGTATATACCCACCCACTGTGC ATAGACAGGATTTCATCCAGGGGCCCACCAGCTTCATGGCAGCCTGCTCTGCAGGAGTCACCACGATGTG TTAGTTAGTATGTGCTAGTCAGGTGGGAGACTCAAGGGTGGAGAGGAGTAGCCTGTGGTTGGTGGCCAAT GCTGCCACCTCAGACCATGCTGCCAGTGAGGGCCATGTCTGAGTCTGTGGCTATGCAGAGGCAAGAATAG GTGTCCATATCTGAGGCTCAATGCCACTAGAGAGCAAGGGAAAACCCTGATGGGGCAGCCTCTCGTGACC ACGTGGCTGTCCAAGGCTGTGCAGAACAGGACCAACCCTTCACTTGCTGCAGCCCTCTAGAGAGCTGGCC CTTCGCCTTGCCCAGGCAACACTGCGGAGCTTGTCCTGGAAGTAGGTGAGTTGGGCCTCTATGTGAGGGA GAGCTGATCCTGCTACTCCTCTGCCATGAGGTGGCCTGGGCAAAGGGTGTGTGAGTGTGGGAGAGCTGAC CCTTCACTTGGCTGTTGTGGGGTGGCTGGGGCACAGAGATGCCTCCCTTGTAGCCTCCACCAGTCAGGAA AGCTGCCTATAGGGTAATAAGTACAGGGAAAACTAGCCTGGCCCCTCACCTCCTGCAGCACTGGGGAGAG TGGGCCCACACCTTGACTGGGCAGAACAGAGCACTTAACTCTGGAGACATGGGTGCTGTCAGCTGACCCT GCCTCTTGCCAGTAGCAGCACTGGGTGGCCTAACCAGAGCAGTGCTGGAGAGCTCGCCCTGGTGAGCAGG TAAGGGAGAGCTGGCAGGCTGAGCAGCTCAGCAGCCCGCAGGCCCAGGTCCAGGGCTCTGAATTTGTCCA TCCCCAAAATCCATACCATCTGCAAATCGTTGGGATGCATGAACAAGCCAGTCCTGCTGTTCCAAAGCTG TAGGAGTCCCGGTGAGGATCCTGTAGTGATGATGTCACAGAAACCAAAGACCTCAAGCCAGACCAATGAC TCATTGCAGTGGACATTTACAAGGAAAGATGTGTGGACAGAAGGTTTACTGTGAGACTCACTGTGACACC ACAGCTTCCTCGATGAAATGTTTCTATGCTTTGCTTTTTATTTGTTGTTTGTTTTTTATTTGGGGGGAGA TTGCAAGGGTAGAGAGTGGATATGAGGGGACAAGGAGATGAGTGGGACTGGGGTGCATGATGGGAAACTC AGAATCAATAAAAACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827521 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827521 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12350 same embryo
	EMAGE:12351 same embryo
	EurExpress:euxassay_008270 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source