EMAGE mouse gene expression spatial database

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EMAGE:12350

Nol6 nucleolar protein family 6 (RNA-associated) ( MGI:2140151)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008267. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008267_01	euxassay_008267_02	euxassay_008267_03	euxassay_008267_04

euxassay_008267_05	euxassay_008267_06	euxassay_008267_07	euxassay_008267_08	euxassay_008267_09

euxassay_008267_10	euxassay_008267_11	euxassay_008267_12	euxassay_008267_13	euxassay_008267_14

euxassay_008267_15	euxassay_008267_16	euxassay_008267_17	euxassay_008267_18	euxassay_008267_19

euxassay_008267_20	euxassay_008267_21	euxassay_008267_22	euxassay_008267_23	euxassay_008267_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12350_wholemount_strong.wlz

12350_wholemount_moderate.wlz

12350_wholemount_weak.wlz

12350_wholemount_possible.wlz

12350_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12350_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9178
Entity Detected:	Nol6, nucleolar protein family 6 (RNA-associated) ( MGI:2140151)
Sequence:	sense strand is shown >T9178 GATCCGGCTTGGATGCCAAGTGCTGGAGAGAGACCTGCTGTGAAGATGGGACCGGCCCCCGCCGGAGAGC AGCACCGACGAGCAACTGAGGACCCAGAGGTGATGGAACTTGCCTTCGAAGGCATGGACAAAGAGAAGGC ACCCTCAAGAAAGCGTGCAAGAACAGAGCCACCTGCAGAAGGCCTCCTGCAGCCAGTGAACCTCAGTCGG GAGGAGCTGTACAAGGAGCCTACAAATGAGGAGCTAAATCGCCTCCGTGAGACCGAGATCTTGTTCCACT CCACCTTGCTTCGTTTACAAGTGGAGGAGTTACTAAAGGAAGTCAGACTGTCAGAGAAGAAGAAAGAGAG GATTGATAATTTCCTGAAGGAGGTTACCAAGCGGATCCAGAAAGTGCCTCCAGTCCCTGAGGCTGAGCTC ACTGACCAGTCATGGCTCCCAGCTGGGGTCCGAGTGCCCCTCCACCAAGTGCCCTATGCCGTGAAGGGCT CCTTCCGCTTCCGGCCTCCATCCCAGATCACTGTTGTGGGTAGCTACCTTCTGGATACTTGTATGCGACC AGATATCAATGTGGACGTGGCAGTGACCATGCCCAGGGAAATCTTACAGGACAAAGACGGGCTGAACCAG CGTTACTTCCGCAAGCGAGCCCTTTACCTGGCCCACTTAGCTTACCACCTGGCCCAGGATCCCCTCTTCA GCAGCGTACGCTTCTCTTACATGAGTGGATGCCACCTGAAGCCCTCGCTGCTGCTGCGGCCACATGGGAA GGATGAGCGCCTAGTTACTGTGCGCCTGCTTCCCTGCCCTCCCTTGGACTTCTTCCGTCCCTGCCGCTTG CTGCCAACTAAGAACAATGTGCGCTCTGCCTGGTACCGAGGGCAGAGCTGTCCGGATTACGAGCCGCCTA CCCCCCACTATAACACCTGGATCCTGCAGGATGTAGCTCTGGAGACCCACATGCACTTGCTGGCAAGTGT GCTGGGCTCTGCCCAGGGCCTGAAGGATGGCGTGGCACTTCTGAAGGTTTGGCTGAGGCAGCGGGAACTG GACAAGGGCCTGGGTGGATTTAACGGATTCATCATCTCCATGCTGGTTGCCTTCCTGGTATCTAAACGAA AGATCCATACCACCATGAGCGGCTACCAGGTCCTGAGAAGTGTCCTGCAGTTTCTGGCCACTACCGATCT GACAATCAATGGAATCAGTTTCTCTCTCAGCTCAGATCCCTCCTTGCCCACCCTGGCTGAATTCCACCAG CTTTTCGCGGTTGTGTTTGTGGACCCATCAGGCCGCCTCAACCTCTGTGCCGATGTTACAGCTTCCACTT ACAACCAGGTGCAGTACGAGGCAGAACTGTCTATGGCGTTGCTGGACAGCAAAGCTGACGATGGATTTCA GCTGCTGTTGATGACTCCCAAACCCATGATCCAGGCTTTTGACCACGTTGTCCATCTCCATCCACTGAGT CGCCTGCAGGCATCCTGTCACCAGCTAAAGCTTTGGCCAGAGCTGCAGGATAACGGAGGGGACTACGTTT CAGCCGCTCTGGGCCCACTAACCAACATTTTGGTGCAGGGTCTGGGGTGCCGGCTGCATCTGCTGGCTCA CTCTCGGCCCCCAGTCCCAGAGTGGAGTATCAACCAAGATCCCCCGAAGCACAAGGACGCTGGGACCCTG ACCCTGGGATTTCTCTTCCGGCCCGAGGGACTGACCAGTGTTATTGATCTTGGCCCAGAGGCTGACAAGC CTGAGGCTGCTGATTTCCGTCAGTTCTGGGGGACCCGCTCAGAGCTCAGGCGTTTCCAGGACGGAGCCAT CCGGGAAGCTGTGGTCTGGGAGGCAGAATCTTTGTTTGAGAAGCGTCTTATTCCTCATCAGGTGGTCACC CACCTCTTAGCACTCCATGCTGACATCCCAGACACCTGTATCCAGTATGTGGGTGGCTTCCTTGATGCAT TGATCCAAAACCCCAAAGAGATCTCCAGCACGGGTGAGGAGGCCTTGGCGTTGGCTGTGCGTTGCTATGA TGACCTCAGCCGCCTGCTCTGGGGGCTGGAGGGTCTCCCACTTACTGTGTCTGCCGTTCAGGGGGCTCAC CCAGTGCTGCGCTACACAGAGGTTTTCCCTCCAGCTCCAGTCCGTCCTGCCTACTCCTTCTATAACCGCC TCCAAGAGCTGGCCTCACTGTTGCCCCGGCCGGATAAGCCCTGCCCAGCCTATGTGGAGCCTATGACTGT GGTTTGTCACCTGGAGGGCAGCGGGCAGTGGCCTCAGGATGCAGAAGCGGTGCAGCGGGTCCGAGCCGCC TTCCAGCTGCGCCTGGCGGAGGTGCTGACCCAGGAGCATAGACTACAGTGCTGCGCCACTGCCACACACA CGGATGTCCTCAAGGATGGGTTCGTGTTTAGGATTCGAGTGGCCTATCAGCGGGAGCCACAGATCCTGAA AGAGGTGCGGAGCCCCGAGGGCATGGTCTCCCTGAGAGACACACCTGCCTCCCTCCGCCTTGAGAGAGAC ACCAAGCTGTTGCCTCTGCTCACCAGTGCCCTGCATGGACTCCAGCAGCAGTACCCAGCCTACTCGGGGG TGGCTCGCCTGGCCAAGCGGTGGGTGCGTGCCCAGCTTCTGGGTGAGGGCTTCACTGACGAGAGCCTGGA TCTGCTGGCCGCTTCACTCTTCCTGCACCCTGAGCCCTTCACCCCTCCCAGCGTCCCACAGGTTGGCTTT CTTCGGTTCCTTTACTTGGTATCTACCTTTGATTGGAAGAACAACCCTCTCATTGTCAACCTCAACGGTG AGCTGACTGCTGAGGAACAGGTGGGGATTCGAAGTAGTTTCCTGGCAGCTCGAACACAACTCCCAGTCAT GGTCATCATTACCCCCCAGGACCGCAGAAGCTCTGTGTGGACACAGGATGGACCCTCAGCCCAGATCCTT CAGCAGCTCGTGAGCCTGGCGGCTGAAGCCCTCCCTATCCTAGAGAAGCAGCTGATGGATCCCCGGGGGC CTGGGGACATCAGGACCGTGTTCCGGCCACCCTTCGACATGTATGATGTGCTGATCCACCTGACTCCCCG CCACATTCCCCGGCACCGCCAAGCTGTAGACCCTCCAGTTGCTTCCTTCTGCCGTGGTCTGCTCGCTGAG CCAGGGCCTTCGTCTCTGATGCCTGTCCTGGGCTATGACCCCCCTCAGCTCTATCTGGCACAGCTCCGGG AGGCCTTTGAAGATCTGGCTCTCTTCTTCTATGACCAGCATGGTGGAGAGGTGATTGGTGTCCTCTGGAA GCCCAGCAGCTTCCAGCCCCAGCCCTTTAAGGCCTCCAGCATAAAGGGACGCATGGTGGTGTCTCAAGGT GGGGAGCTTGTGATGTTGCCCAACATAGAAGCAATCTTGGAGGACTTTGCTGTGCTGGGTGAAGGCCTGG TACAGGCTGTAGAGGCCCGAAGTGAGAGGTGGACTGTGTGATTCTCGCCTTGGGACAAGTGTTGGATCTG TGCAGCAGCAGGTCAGCGGCATAGCCACTCTCAGTGCAGATGGACGCCTGATGAACCACTGAATCCCCCA TCCCCAGAGAAAGCCCCGTGAGATTCTTCTGATGTTTCAGCTCTGGGGCAGGGGCCATGGTGTCCTGTGT CCCCTACACCTGACCACCCCAATTCCCAGAGGAGGAGACAGAGCCAGCTCAGAGAGAACTGAATCCACGA GATCCATTTGCCTATCCATCTTGAGTCAGCCTGAACTCTTGTTCATTTTTGTTTTCTCTCCATCTTCATC TTCCCGCTGGCAGGACGGATGTGTCCAGATGTCAGCGTGCCTTTCCCTGTTGCTGCTTCTGGCCTTTAGA TTGTGGCCCTTCCTGGGGGTCAGGGCACCAGCACTGAGCAGTGGGAGGGAGCTGTGTGTGTATGAATGGA ACCCATTCAGTAGGCACAGTGCCCTGAGGGTAGGAACAGTGGGAAAAGATACAGACCAGGCAGGGCTGGC TGGAGTTGAGGGGATCCCGGTCCAAGAGAGAGAGAGCGCTGGGCGCTGCAGCCTGGCCTCTCTGGGTCCT GCCCCCTCCTTGCTGCTGCCTATCATTACCCATTACTGTGCCATACGCCTGCTCTTGGAACTCTGAGGTA GGCAGGATGCTTCCTCTCAGCCTGATCCACATCCCCTTCTGGCAGCCACTCCCTGCTTTTCTCCCCTAAT CCTGACTGTTTTTTTTTTTAGACTCAGCTCAAATAGCACCCCACCTAGTCCCTGCCCTCACCCCCACCCC TCTCTCCCTTCTGTGAACTGTGAATGCGATTATTCCTTGTCGAGTTCACTTGGCAGGCATGCATACCAGT ATGCACCTTCTATTGTCCTGAACTCTGATTTAAAATTAAATTGCTTAAGTCTTTCTGGGTAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827596 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827596 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12349 same embryo
	EMAGE:12351 same embryo
	EurExpress:euxassay_008267 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source