EMAGE mouse gene expression spatial database

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EMAGE:12353

Smc2 structural maintenance of chromosomes 2 ( MGI:106067)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011062. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011062_01	euxassay_011062_02	euxassay_011062_03	euxassay_011062_04

euxassay_011062_05	euxassay_011062_06	euxassay_011062_07	euxassay_011062_08	euxassay_011062_09

euxassay_011062_10	euxassay_011062_11	euxassay_011062_12	euxassay_011062_13	euxassay_011062_14

euxassay_011062_15	euxassay_011062_16	euxassay_011062_17	euxassay_011062_18	euxassay_011062_19

euxassay_011062_20	euxassay_011062_21	euxassay_011062_22	euxassay_011062_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12353_wholemount_strong.wlz

12353_wholemount_moderate.wlz

12353_wholemount_weak.wlz

12353_wholemount_possible.wlz

12353_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12353_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 17 18 19

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 09 21 22 23

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 12 15 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22

rest of cerebellum marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 14 16 17 18 19 20 21

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 14 16 17 18 19 20 21

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

dorsal grey horn

strong

regional

strong expression: see section 14

ventral grey horn

strong

regional

strong expression: see section 13 14

viscerocranium

moderate

regional

Expression in the turbinate bone.

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 15 16

lower jaw molar

moderate

regional

moderate expression: see section 07 20

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13 15 16 17

upper jaw molar

moderate

regional

moderate expression: see section 07 19 20

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

renal cortex

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20

lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9448
Entity Detected:	Smc2, structural maintenance of chromosomes 2 ( MGI:106067)
Sequence:	sense strand is shown >T9448 CAAAGGAACAGTTCCGGCACGGTTGTTGGCTGCAGCGGATGCGGTGCCCGGGATGGTGGCGTTCGCCCGG TAGCGCCTGAGTACAGACGTGACTTGTAGCTTGTTGCCTAACAGGCTTACTAACCCAAGACAGTTTAGAA AATGTATGTTAAATCAATCATTCTCGAAGGATTCAAGTCCTATGCACAGAGGACTGAAGTCAATGGTTTT GACCCCCTCTTCAATGCGATCACTGGTTTAAATGGTAGTGGAAAATCCAACATATTGGACTCCATATGCT TTCTCCTAGGCATCTCCAACCTGTCCCAGGTGCGGGCTTCAAATTTACAAGATCTCGTTTATAAAAATGG ACAGGCTGGGATTACCAAAGCCTCTGTTTCAATCACTTTTGATAATTCTGACAAAAAGCAGAGTCCGTTG GGATTTGAAGCTCATGATGAAATCACAGTAACAAGACAGGTGGTTATTGGTGGCAGAAATAAATACTTAA TCAATGGAGTGAATGCCAACAACACCAGAGTCCAAGATCTCTTTTGTTCTGTGGGCCTGAATGTTAACAA CCCTCACTTTCTCATCATGCAGGGCAGAATTACGAAAGTATTAAATATGAAACCACCAGAGATATTATCC ATGATTGAAGAAGCTGCTGGAACCAGGATGTATGAGTACAAAAAAATAGCCGCCCAGAAAACTATAGAAA AAAAGGAGGCTAAGCTGAAAGAAATAAAAACGATACTGGAAGAAGAGATTACTCCAACGATTCAAAAATT AAAAGAGGAAAGATCTTCTTACTTGGAGTACCAGAAAGTAATGCGGGAGATAGAGCATCTGAGTCGTTTA TACATTGCCTATCAGTTTTTGCGGGCTGAAGACACCAAGGAACGCTCAGCTGGGGAGCTAAAGGAAATGC AGGATAAAATAGTAAATCTTCAAGAAGTACTGTCAGAGAATGAAAAAAAAATAAAGGCTCTCAATTGTGA AATCGAAGAATTGGAGAGAAGAAAAGATAAGGAAACTGGAGGCAAACTCAAGTCTCTAGAGGATGCTTGT GCAGAAGCCCAGCGAGTTAATACTAAGTCTCAAAGTGCATTTGACCTCAAGAAGAAAAACCTAGCTAGTG AAGAAACCAAGCGCAAAGAGCTACAAAACAGTATGGCTGAGGATTCTAAAGCATTAGCAGCAAAGGAAAA AGAAGTTAAGAAGATAACAGATGGACTGCATGGCCTTCAAGAAGCAAGCAATAAAGATGCTGAAGCTTTG GCTGCTGCACAGCAGCACTTCAATGCTGTTTCTGCTGGCTTGTCCAGTAACGAAGATGGAGCAGAAGCAA CTCTGGCTGGGCAGATGATAGCCTGTAAAAACGATATCAGCAAAGCTCAGACTGAGGCCAAGCAGGCTCA GATGAAGCTGAAACATGCCCAACAAGAACTAAAGAGTAAGCAAGCAGAGGTTAAGAAGATGGACAGTGGC TACAAGAAGGATCAAGACGCTTTTGAAGCTGTGAAAAAAGCTAAAGAAAAACTTGAAACTGAAATGAAGA AGCTAAATTACGAAGAAAACAAAGAGGAAAAGCTTTTGGAAAAGCACAGGCAGCTGTCCCGTGACATCAA CAACCTAAAAGGAAAACATGAAGCTCTCTTGGCCAAGTTTCCCAATCTTCAATTTGCATACAAGGACCCA GAGAAGAACTGGAATCGAAATTCTGTGAAAGGACTTGTAGCTTCTCTGATAAATGTGAAAGATAATTCTA CAGCCACAGCTCTGGAAGTGGTGGCTGGGGAACGACTCTACAATGTTGTAGTAGACACAGAGGTAACTGC TAAGAAGCTACTAGAAAAGGGGGAGTTGAAGCGTCGATACACAATAATTCCTCTCAATAAGATTTCAGCC AGATGTATTGCTCCTGAAACTCTGAGGGTCGCTCAGAATCTTGTTGGTCCTGACAATGTTCATGTAGCTC TTTCCTTGGTTGACTATAAACCAGAACTTCAGAAAGGAATGGAATTTGTCTTTGGAACAACATTTGTTTG TAACAATATGGATAATGCCAAAAAAGTGGCCTTTGATAAAAGGATAATGACCAGAACTGTAACCCTTGGG GGTGATGTATTTGATCCTCATGGAACATTGAGTGGAGGTGCTAGGTCCCAGGCAGCTTCGATTTTAACCA AGTTTCAAGAAGTCAAAGATGTTCAAGATGAGCTGAGGACCAAGGAGAATGAACTGCGCGCTCTAGAAGA GGAGTTAGCAGGTCTTAAAAATGTTGCTGAAAAGTATCGCCAACTGAAACAGCAGTGGGAGATGAAGACA GAGGAGGGAGACTTATTGCAAACTAAACTTCAGCAAAGCTCTTATCATAAGCAACAAGAAGAATTAGATG CCCTTAAAAAAACCATTGAGGAAAGTGAGGAGACATTAAAAAGCACAAAAGAAATCCAAAAGAAAGCTGA AGAAAAGTATGAAGCATTGGAGAATAAAATGAAAAATGCGGAAGCTGAAAGAGAGAAAGAACTGAAGGAT GCTCAGAAAAAACTAGATTGTGCCAAAACAAAGGCAGATGCATCTAGCAAGAAAATGAAAGAAAAACAAC AGGAGGTTGAAGCTATTACTCTAGAGCTGGAGGAGCTCAAAAGAGAGCATGCTTCTAATGAGCAGCAGCT TGATGCCGTGAATGAAGCCATCAAAGCCTATGAAGGTCAGATTGAAAAAATGGCAGCTGAGGTCGCTAAA AATAAGGAATCAGTAAATAAAGCTCAAGACGAGCTGATGAAGCAAAAGCAAATAATAACAGCCCAGGACA ACATAATTAAAGATAAATGTGCAGAAGTGGCGAAACACAATTTGCAAAACAATGAGTCTCAACTTAAAAT TAAGGAGTTGGACCACAGCATAAGCAAACATAAGCGGGAAGCGGATGATGCTGCTGCAAAGGTATCCAAA ATGTTAAGTGATTATGATTGGATTAATGCTGAGAAACACCTATTTGGCCAACCTAATAGTGCCTATGATT TCAAAACAAACAACCCCAAAGAAGCTGGCCAGAGACTTCAGAAGTTACAGGAAGTAAAAGAGAAACTAGG GAGAAATGTCAACCTGAGAGCTATGAACGTGCTGACAGAAGCTGAAGAGCGGTATAATGATTTGATGAAG AAGAAAAGAATTGTAGAAAATGACAAATCCAAAATCCTTGCAACTATAGAAGATCTTGACCAGAAGAAAA ACCAGGCCCTAAATATTGCTTGGCAAAAGGTGAACAAAGACTTTGGCTCTATTTTTTCTACCCTTTTGCC TGGTGCCAATGCTATGCTTGCACCACCAGAAGGGCAGACAGTTTTAGATGGTCTGGAGTTCAAGGTTGCC TTAGGAAATACGTGGAAAGAAAACCTAACTGAGCTTAGTGGTGGTCAGAGGTCTCTAGTGGCTCTCTCAC TAATATTGTCCATGCTCCTCTTCAAACCTGCTCCAATTTATATCTTGGATGAGGTGGATGCGGCCTTGGA TCTTTCTCATACCCAGAATATTGGGCAGATGCTGCGGACTCACTTCACACATTCTCAATTCATTGTGGTA TCCCTCAAAGAAGGTATGTTCAACAATGCAAACGTTCTCTTCAAAACCAAGTTTGTGGATGGTGTTTCTA CAGTAGCCAGATTTACGCAGAGTCAAGCTGGGAAGATTCCGAAGGAAGCAAAGTCCAGAGGAAAAGAACC CAACTGAGTGCATGCCAGTTCAGAGTACAGACTTCTCACCTTGACGTTTTTACACATGAACTTCCAAGAA TTTGGGATCCAAGTTGTTTATATAATAATGTTAACATTATTTTCATATGTAATTCTTTTTAGCACTTTTA AAATTGCAGATTCCTCTTTTCTTACCTAGTGTGATTATGGTCCAATTATTGTAGTTGTGATTGCATGTAT ATCATCAATTTTTTTCTTACAAAAATCTTTTGTATGTATTAAAGAACCTGAGGTACATATAGTTGGTAAA TTGTGTATGCAAATGCCCAAAATACAAAGAAACAGATGAAGTTATAGGATAAATATAGCTGACAGTCAGC TTTTAACTAGTTTAATTTCTAAGTACAGTATTAATGACTTTTCTTTTGCTTATAAAATCATTCAGATACA GAATAGTTGTAAAGAAAGTCACTGGACCATTAAAGGGAACACATAGCAATTATGCATACACCGAGCTACT TGAGGTCAGTCCTGTGGAGACAGCATGCCAGATCCTTGGCTGGGAAATGGATCTGGAGAGACTTTCACGT TAGTGAGGTTGGACTATCATGTTTATGTATGTTTAATAATTGATCTCCACAAGCTTGAAATTTGAGTGTT TTTTAGCTTTGAGTATTTTAGTAGGAGCTTGGGAAGAAATGTGTTATTACACAGTATTACACTTGGATTA TTCTATCTACAGTCCTGTAATATTTTGTAAGATTTCTATCAGACTTCTCATAATCTAATCAATAATTTTT TGGTGAATGTAGGACCTAGCAGATTAAATGAAAAGTATATTTTATAAGAGTCTGGTAAAGGTAACTAAGT CTGTCTTCTCTACCAAAAAAACCTCCAAAGCTTCATGATTGATGAAATTCTTGGTGCCTTCTGCTTGTCT GGGTCAAAGGTAAAACTCCTTTAGAAAGCAGGAGGGGCCCAGTACACACCCTGAGATCCTGCAGCTCTTA CTGTCTCTACGTACATTTCCACATCAGCACCTCATGCCCGTAATCCTCAGAGCGCTAATGTGATCTGATT GGATTGGACAGATGGGGCCCATGTTTCGTCCTGATACTTAACCCTTAGTTCCTTGATGTTCTACTTTCTT TGTTGTGTCATACAGGCCCTGAGTTGGACATTTCTCAAACTTATATATGTGGTGTTATATAGACCTTAAG TCATTTGTACCCTGGAACATTTTGGAAATTATTTCATCTGGAGGGAAGTGGCAGTAGGCTGAAAATACTG ACTGATGCATAGTAGAAATCTCAGATAATGGACCTACACAATGTTTTGTTTTCCAAGGGGTTATAAACTA TTGGTCAAAATAGTTGTGTTTATTTACCCTGTCTCTAATAGTGGTATTCTAATTTGATCACAATCTAGTC AATGCCAGCTGTTAATATTTTGTATTAGGCAATTATAAAATAGCGTTGAATTGCTGTCTTAAGCTTATAA GTGACTATTTTGTGTGATTTCCTTTGTCTATAAATTATTTCCTTGTATGCCTTTCTGTTTTGTTTTCTTT TGTTTTTGTATTTTATATATAGTTTCACTCATATTTTTGTGCATATATTAAATTTCTGTGAGACATGCTA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30543190 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30543190 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12354 same embryo
	EurExpress:euxassay_011062 same experiment
	MGI:4828325 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source