EMAGE mouse gene expression spatial database

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EMAGE:12354

Kif5b kinesin family member 5B ( MGI:1098268)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011057. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011057_01	euxassay_011057_02	euxassay_011057_03	euxassay_011057_04

euxassay_011057_05	euxassay_011057_06	euxassay_011057_07	euxassay_011057_08	euxassay_011057_09

euxassay_011057_10	euxassay_011057_11	euxassay_011057_12	euxassay_011057_13	euxassay_011057_14

euxassay_011057_15	euxassay_011057_16	euxassay_011057_17	euxassay_011057_18	euxassay_011057_19

euxassay_011057_20	euxassay_011057_21	euxassay_011057_22	euxassay_011057_23	euxassay_011057_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12354_wholemount_strong.wlz

12354_wholemount_moderate.wlz

12354_wholemount_weak.wlz

12354_wholemount_possible.wlz

12354_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12354_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9430
Entity Detected:	Kif5b, kinesin family member 5B ( MGI:1098268)
Sequence:	sense strand is shown >T9430 GGCCTTTGGAGGGTGCGCGCGTCAGCTGGCCGGGAAGCCGGCGAGCTGCCGGGACAGGATAGTGGGCCGA GCCCTAGGCCTCGGCTGTCCTTCCCGAAAAGAAAAGAAGGAGCCGCTCGCCAGTCCCCGCAGCCTGTGGA GGCCGGTGAGGGAGACGCCGAGGAGCATCGAGGCTCTGTGGGCGCCGCCGCCTTGGCCGCCATGATGGAG CGGAAGTGAACTTCGGGCGCAGGCTGCCGGCGACAGCGCTTCGGTCTAGCTGCCCGGGGTGCACGGCGCT GCGGAGCCCGGACCTTCCGGTCCTCGCGACCCCTGCCTCTTGGCTCCCCTCGCTTACCCCAAGACTGAGC GGCTTGAAGAGGCGCCCGGCCTGCGGTCCCGGCTTCCAGAAAGATGGCGGACCCGGCGGAGTGCAACATC AAAGTGATGTGTCGCTTCAGACCTCTCAACGAATCTGAAGTGAACCGCGGCGATAAGTACGTCGCCAAAT TCCAGGGAGAAGACACGGTGATGATCGCGTCCAAGCCTTATGCCTTTGATCGTGTGTTCCAGTCAAGCAC ATCTCAAGAGCAAGTATACAACGACTGTGCAAAAAAGATTGTTAAAGATGTTCTTGAGGGCTATAATGGA ACAATATTTGCATATGGACAAACATCATCTGGGAAGACCCACACGATGGAGGGTAAACTTCATGATCCAG AAGGCATGGGAATTATTCCAAGAATAGTGCAAGATATTTTTAATTATATTTACTCCATGGATGAAAATTT GGAATTTCATATTAAGGTTTCATATTTTGAAATATATTTGGATAAGATAAGGGACTTGTTAGATGTTTCA AAGACTAACCTTTCAGTCCATGAAGACAAAAACCGTGTTCCCTATGTAAAGGGGTGCACAGAACGTTTCG TGTGTAGTCCAGATGAAGTCATGGATACCATAGATGAAGGGAAATCCAACAGACATGTCGCAGTTACAAA TATGAATGAACATAGCTCTAGGAGTCACAGCATATTTCTTATTAATGTAAAACAAGAGAATACACAAACG GAACAGAAACTCAGTGGAAAGCTTTATCTGGTTGATTTAGCTGGCAGTGAGAAGGTTAGTAAGACTGGGG CTGAAGGTGCTGTGCTGGATGAAGCTAAGAACATCAACAAGTCACTTTCTGCACTTGGAAATGTCATTTC TGCTTTGGCAGAGGGCAGTACCTATGTTCCTTATCGAGATAGTAAAATGACCAGAATTCTTCAAGATTCA TTAGGTGGCAACTGTAGGACCACTATTGTCATATGCTGCTCTCCATCATCATACAATGAGTCTGAGACAA AGTCAACACTCCTCTTTGGTCAAAGGGCCAAAACAATTAAGAACACAGTCTGTGTCAATGTAGAGTTAAC TGCGGAGCAGTGGAAAAAGAAGTATGAAAAAGAAAAGGAAAAAAATAAAACTCTACGGAACACTATTCAG TGGCTGGAAAACGAGCTAAACCGTTGGCGTAACGGGGAGACAGTGCCTATTGATGAGCAGTTTGACAAAG AGAAAGCTAATTTGGAAGCCTTCACAGCGGATAAAGATATTGCTATTACCAGTGATAAACCAGCTGCTGC AGTCGGAATGGCTGGTAGTTTTACCGATGCTGAAAGAAGAAAGTGTGAAGAAGAACTTGCTAAATTGTAT AAACAACTTGATGACAAGGATGAAGAGATTAACCAACAAAGCCAATTGGTAGAGAAATTGAAGACACAAA TGCTGGATCAGGAAGAGCTTCTGGCATCAACCAGAAGGGATCAAGATAATATGCAAGCTGAACTGAATCG CCTCCAAGCAGAAAATGATGCTTCTAAAGAAGAAGTCAAAGAAGTTTTACAGGCCTTAGAGGAACTGGCT GTTAATTATGATCAGAAGTCTCAGGAAGTTGAAGACAAAACAAAGGAATATGAATTGCTTAGTGATGAAT TGAATCAAAAATCTGCAACTTTAGCAAGTATTGATGCTGAGCTTCAGAAGCTGAAGGAAATGACCAACCA CCAGAAGAAACGAGCAGCTGAAATGATGGCATCATTATTAAAAGACCTTGCAGAAATAGGAATTGCTGTG GGGAATAACGATGTGAAGCAACCAGAAGGAACTGGTATGATAGATGAAGAGTTTACTGTTGCAAGACTCT ACATTAGCAAAATGAAATCAGAAGTAAAGACCATGGTGAAACGCTGCAAACAGCTAGAAAGCACACAGAC TGAGAGCAACAAAAAAATGGAAGAAAATGAGAAAGAGTTAGCAGCATGCCAGCTTCGGATCTCCCAACAT GAAGCCAAAATCAAGTCACTGACTGAGTACCTTCAGAATGTAGAACAAAAGAAGAGGCAGCTGGAGGAGT CTGTTGATTCCCTTGGTGAGGAGCTAGTCCAACTCCGAGCACAAGAGAAAGTCCATGAAATGGAAAAAGA GCACTTGAACAAGGTTCAGACTGCAAATGAAGTCAAGCAAGCTGTTGAGCAGCAGATCCAGAGTCACAGA GAAACCCACCAAAAACAAATCAGTAGTTTGCGAGATGAAGTGGAGGCAAAGGAAAAGCTAATCACTGACC TCCAAGACCAAAACCAGAAGATGGTGTTGGAGCAGGAACGGCTAAGGGTGGAGCATGAGAGGCTGAAGGC TACAGACCAAGAGAAGAGCAGGAAGCTGCACGAGCTCACGGTTATGCAAGACAGACGAGAACAAGCAAGA CAAGACTTGAAGGGTTTGGAGGAGACCGTGGCAAAAGAACTTCAGACTTTACACAACCTGCGTAAGCTCT TTGTTCAGGACTTGGCTACCAGGGTGAAAAAGAGCGCCGAGGTCGACTCTGACGACACTGGCGGCAGTGC TGCACAGAAGCAGAAAATCTCCTTCCTTGAAAACAACCTTGAACAGCTCACCAAAGTGCACAAGCAGTTG GTACGCGATAATGCAGATCTTCGCTGTGAGCTTCCTAAGTTAGAGAAACGGCTTAGAGCTACTGCAGAAA GAGTGAAAGCTTTGGAGTCAGCCCTGAAAGAAGCCAAAGAAAATGCATCTCGAGACCGTAAACGCTATCA GCAAGAAGTAGACCGGATAAAGGAAGCAGTCAGGTCAAAGAACATGGCCAGAAGGGGACATTCTGCCCAG ATTGCAAAGCCGATCCGTCCTGGACAGCATCCAGCGGCCTCGCCAACTCACCCGGGCACAGTTCGTGGAG GAGGCTCATTTGTTCAGAACAACCAGCCAGTGGGGCTTCGTGGTGGTGGAGGCAAGCAGTCGTAAACACT TGACACATTGCAGGTGTTGAAAAGTAATTGAAGTTTGAAGAGGACATAAAATCAGTCTTTCACTAACTAA CCTCTATTCCTTGGGATTGTAGAAATTTAATTTTTTAATGTGTAAATTACTCCTACCCTGTACAGCTGTT TCCTACCCACTCTTGTAAACTCTGCTGCTTCCCAACACTAGAGTGCAATGTTGATGTCCTAGAAGGGAAA CGACAGTTTACAACTGCGGCCCTGTTTGTTACACGGTGTGTCCTTCGTGTCTTCACTGTGCTGAGTAACT GTGTTGAGGGACTCTGCTCCTTGATTTGCCTTTCTGTGAGTGTGTAATCTTGGTTTACATTACATAGCTG CTATTGCTTTTCATTTTCCCTTTTCTACTAAAATGTCTTTTTTTTTTTAAAGGAAATATGGAACATTTAG ATTAAATGTCTTAAATTTTACTACTTATAACACTACTTGCCACAAAATATATCAGGTCAGTCAGTACTGT ATTTTAAAAATCCCTCAAGCAAGGTATGGTGGCACGCATCTCTACTCCCAGGCAGAGGCAGATGGATGGC TGTCAGCTTGAGTTCAGCCTGCTTTCCAGGACAGCCAGGGCTATATAGGGAGACCCTGTCTCAAAAATAA ATCAAAAAATAAAAAATTAACTTAAAAATAAATTTAAAAATCCCTTGATATGTTGCTGGTATAGTCTCTG AAGCTTGCCTCTGAAAACGTTGAGCTTTGAGACCAGGAGTTCGAGACTGAACAGGCTCTTTCTATTATAT CATTAGCTCATGAGATGCCTTTTGAATACAAATATCTTACATATCAAAAATGGATCTCATGTTAAATGGA TTTGGAAGTACTATACCCCTTCTTTCTACTTCTGCACTTAAACATAGCTTAATGTGCTCTGCTTTGTTCT GTTATATCTTCCTGCTAAAGTAAGAATTGAGCAGAGCTAATGTCATCTTTAATATTAGGAAATAAGTGGA CTAAGACATTATAGAATTAAATAATTTTCAAATGTTAAATAATTGTATTGACTGATAAATTTATAGCCTC TGAAAAATGAGAAAAAAATTGTGAACAGGACAGATGAAGTATAAATTTAAAACTACATGGTTAATTTTGC TCTGAAATCCTATAGGTGGTTTTCACCTGTTATACTAAATCATACACTATAATTTGTTTAGTTAGAAATA GTAAACTGTTTGCAAGTCACTAGTAATTCTCAAAAAAATATTGTTGGCCATAGTGAATAATTTTTAAATT AGAACTAAGTGTGTACCTATTTTTGGCACTTATTTTTCAAATAATTAAGCTCAATCAAGTATATTTTAAG GATCTATAAAAGTGACAGGAAGACCGGGTGTGGTGGCGCACACCTTTAATCCCAGCACTTGGGAGGCAGA GGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAAGACAGCCAGGGCTACAC AGAGAAACCTTGTCTCGAAAAACCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30543821 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30543821 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12353 same embryo
	EurExpress:euxassay_011057 same experiment
	MGI:4825768 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source