EMAGE mouse gene expression spatial database

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EMAGE:12363

Dnahc12 dynein, axonemal, heavy chain 12 ( MGI:107720)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011149. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011149_01	euxassay_011149_02	euxassay_011149_03	euxassay_011149_04

euxassay_011149_05	euxassay_011149_06	euxassay_011149_07	euxassay_011149_08	euxassay_011149_09

euxassay_011149_10	euxassay_011149_11	euxassay_011149_12	euxassay_011149_13	euxassay_011149_14

euxassay_011149_15	euxassay_011149_16	euxassay_011149_17	euxassay_011149_18	euxassay_011149_19

euxassay_011149_20	euxassay_011149_21	euxassay_011149_22	euxassay_011149_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12363_wholemount_strong.wlz

12363_wholemount_moderate.wlz

12363_wholemount_weak.wlz

12363_wholemount_possible.wlz

12363_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12363_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon roof plate

moderate

regional

moderate expression: see section 13 weak expression: see section 12

choroid invagination

moderate

regional

moderate expression: see section 08 09 10 14 15 weak expression: see section 16

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 05 06 07 08 09 10 11 12 16 17 18

nasal cavity olfactory epithelium

moderate

spotted

moderate expression: see section 08 09 11 12 16

pharynx epithelium

moderate

spotted

moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37352
Entity Detected:	Dnahc12, dynein, axonemal, heavy chain 12 ( MGI:107720)
Sequence:	sense strand is shown >T37352 CTATGGTTCGTTTGGACTGTGAAGACTTGAAGATAGGCTTGACAAATAAAGCAAGAGCCTTTGCAAACAT TTTATTAAATGACATAGCTTCAAAACATAGAAAAGAAAATGAATCTATTTGCAGTGAATTTGAAGCAATT AGAGACCATGCGCTACGAGTTCCTGAGACCACAGAAGAAATGATGGAGCTAATTGCTTTCGTAGAAAGAG CTCGAACCGTAGGAATTCTTGACTTGGCTCTAAGGATCCAGGAATCTAAACGCCAAATGAGTTACTTTTT AGATGCTCTTCTAATGTCTCAAGAAGACTTAAATTTAAATGCAACTGTCCTCTTGTGGCCTACTAAAATT ACCCCTGTCTTTGATGAGAATGATGAACTCATTGAGAATGCTAAACATGCAAAAGAAAATGAACTAATAG CCAAGAGAGAAAAACTGATTTTGGAAATAGAAAAAGAATCACGTCGCATGGAGGAGTTCACCGAGTTTGC AGAACTGGACCGCATGCATCAGTATGTGGCAGATGTAAGACAACTGCAGAAACGGATTCAGGAATCGGAA GAAGCTGTTCAGTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84531. Forward Primer - name:084531_F_cDNA_4921531P07Rik, sequence:CTATGGTTCGTTTGGACTGTGA; Reverse Primer - name:084531_N_SP6_cDNA_4921531P07Rik, sequence:AAACTGAACAGCTTCTTCCGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12361 same embryo
	EMAGE:12362 same embryo
	EMAGE:12365 same embryo
	EMAGE:12364 same embryo
	EurExpress:euxassay_011149 same experiment
	MGI:4824338 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source