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EMAGE:1237

Mitf microphthalmia-associated transcription factor ( MGI:104554)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:1237
Fig 4D Martinez-Morales et al, 2001 [PMID:11493524] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: ol, outer layer; ov, optic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:1237EMAGE:1237Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1237_voxel_strong_3D_1.wlz
1237_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1237_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
optic vesicle
detected detected
regionalExpression is in the presumptive RPE (retinal pigmented epithelium).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2137294
Entity Detected:Mitf, microphthalmia-associated transcription factor ( MGI:104554)
Notes:The anti-Mitf antibody used in this study by Martinez-Morales et al, 2001 [PMID:11493524] is described as a "monoclonal antibody against MITF (Neomarkers)", "used at 1:1000 dilution".
Antibody Type:monoclonal
Raised In:mouse
Supplier:Neomarkers
Specimen
Organism:mouse
Strain:DBA/2 x C57BL/6
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:either cryosection or paraplast
Secondary Antibody:goat anti-mouse biotin-conjugated IgG
Tertiary Antibody:peroxidase-conjugated streptavidin
Labelled with:horse radish peroxidase
Visualisation method:NovaRed substrate (Vector Laboratories)
General Information
Authors:Martinez-Morales et al, 2001 [PMID:11493524] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11493524] Martinez-Morales JR, Signore M, Acampora D, Simeone A, Bovolenta P 2001 Otx genes are required for tissue specification in the developing eye. Development (128):2019-30
Links:MGI:2137358 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI