EMAGE mouse gene expression spatial database

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EMAGE:12378

Rit2 Ras-like without CAAX 2 ( MGI:108054)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007060. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007060_01	euxassay_007060_02	euxassay_007060_03	euxassay_007060_04

euxassay_007060_05	euxassay_007060_06	euxassay_007060_07	euxassay_007060_08	euxassay_007060_09

euxassay_007060_10	euxassay_007060_11	euxassay_007060_12	euxassay_007060_13	euxassay_007060_14

euxassay_007060_15	euxassay_007060_16	euxassay_007060_17	euxassay_007060_18	euxassay_007060_19

euxassay_007060_20	euxassay_007060_21	euxassay_007060_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12378_wholemount_strong.wlz

12378_wholemount_moderate.wlz

12378_wholemount_weak.wlz

12378_wholemount_possible.wlz

12378_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12378_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 weak expression: see section 17 18

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 16 weak expression: see section 07 17 18 19

diencephalon lateral wall marginal layer

moderate

regional

moderate expression: see section 15

telencephalon mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 11 12 13 14 15 16 19 20 21 22 weak expression: see section 01 02 03 09 10 17 18

telencephalon marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 12 13 16 19 20 21 22 weak expression: see section 01 02 03 09 10 17 18

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 04 05 06 15 16 17 18

pons mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 weak expression: see section 05 06 07 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

spinal cord mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

neural retina

weak

regional

weak expression: see section 02 03 04

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 18 19 22 weak expression: see section 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4321
Entity Detected:	Rit2, Ras-like without CAAX 2 ( MGI:108054)
Sequence:	sense strand is shown >T4321 CAATTGCAGTGGTTGCTATGAGACCCGCTCCAGCAGAGGACCAGCAGGCAGCCTGTCGCTGATGGTTCTT GTCGTGCACCAAAGCTTTCCTTTGACACAGTTTTAGAGTTGTTTACTATCTGAGCAAGCACCTGACACGG TGACTCTTTTCTTCCTTTTTTTCCTCCTCGGGTCCCCCGAGTAAGATGGAAGTAGAAAACGAAGCCCACT GCTGCCCTGGCAGCTCATCAGGCGGGTCCAGAGAGTACAAGGTGGTAATGCTGGGCGCAGGGGGCGTTGG TAAAAGCGCAGTCACAATGCAGTTTATAAGCCACCAGTTCCCGGACTATCACGACCCCACAATCGAAGAT GCTTATAAAACCCAGGTGAGGATTGATAATGAGCCTGCTTACTTAGACATCTTGGACACTGCTGGTCAGG CAGAGTTCACGGCCATGCGGGAGCAGTACATGCGTGGGGGAGAGGGCTTCATCATCTGCTATTCTGTCAC TGACCGCCAGTCATTCCAGGAGGCTGCCAAGTTCAAGGAGCTTATTTTCCAGGTCCGTCACACCTATGAA ATTCCCCTTGTGCTAGTGGGTAACAAAATTGACTTGGAGCAGTTCCGTCAGGTATCTACAGAAGAAGGCA TGAATCTTGCTCGAGACTACAACTGTGCCTTCTTTGAGACATCTGCAGCCCTGCGATTCGGTATCGATGA TGCTTTTCAAGGCTTAGTGAGAGAAATTCGCAGGAAGGAATCCATGCTGTCCTTGGTGGAAAGGAAATTG AAGAGGAAGGACAGCCTGTGGAAGAAGATAAAAGCCTCCCTGAAGAAGAAGAGAGAAAACATGTTGTGAG GCTTTGCTTCCGCATCCTTCCTGTCTCTCCTTGTGGGTTTTATCAACCAGACAATTGTTCATATCTCCAT CTCACTCTCTCTTTCTCTCTCCATCTCTTTTTCTTTCCCTTTCCCCCTCTCTTAACACACACACACACAC AATCTACCTGCAGGCAGAAATTAGACTTCTATAACTGTGTGTCTTGAGATAGTTTTATTTTGCCTTTAAC AGCTGGATAGATTTTGTCAATCAGCTGGATAATGCTTTATAGATTTTACAATATATTACCAAATAAAATT AACATTCCAATTGCCTCATTTTCCTGTAAAGTGTAGCTTCATTTTGCATGCTGTCTGGTTATAAAGAAAA TCTGCAAACTTGTAACTGGGATGATAAAAGGCTTAGGGGATCTGCTTTTGCCTGATAACCCTGGTTGTTT GCATTTCACAAATCTGTAAATAATGAAGAGGCTGGAATAGTTTAGAGGAAAAAAAAATGTCCTTGTCTTC TCTTAAGTGGAAGAGTCTTGGTATTTCTTCTTGAGTCCTCTATAGATGAACATTAGAGAAATTATTGATA ATAGGAACTGAGTTTGTGTTATTAAAATACTAAAATTACATGTCCCCATATTTAGTGCTCAGTCTGCCAT ATGCTTTATAGAAGTACCACAGTAAAGTTACTGTAATGATAGTCTATGTTATCTGTTGTAGGAAGACAGC AATACTATGAACTAGATGATACATGTGCCCAAGAAAGGACCCTTTGCCCCTCTCAATGAATCTTCTTAGA ATTGCTTCACCTGCATAGTAGCCATCTGTCTGCAGCGATATGGCCTCTACATACTAAGTGTTCTCTTCAT ATTATTAAATTTCCTCTCTCATTTTCCAAGAAGCATTACAAGAAGCTGACATCAGAAATGTTGTTGTTAT TTGTCTTGTTATTTGTTCATTGTGTAAAATAAAATCTCTCAGTCACCCATTTAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4511264 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4511264 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12377 same embryo
	EurExpress:euxassay_007060 same experiment
	MGI:4827750 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source