EMAGE mouse gene expression spatial database

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EMAGE:12379

Pcdha7 protocadherin alpha 7 ( MGI:1298369)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008269. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008269_01	euxassay_008269_02	euxassay_008269_03	euxassay_008269_04

euxassay_008269_05	euxassay_008269_06	euxassay_008269_07	euxassay_008269_08	euxassay_008269_09

euxassay_008269_10	euxassay_008269_11	euxassay_008269_12	euxassay_008269_13	euxassay_008269_14

euxassay_008269_15	euxassay_008269_16	euxassay_008269_17	euxassay_008269_18	euxassay_008269_19

euxassay_008269_20	euxassay_008269_21	euxassay_008269_22	euxassay_008269_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12379_wholemount_strong.wlz

12379_wholemount_moderate.wlz

12379_wholemount_weak.wlz

12379_wholemount_possible.wlz

12379_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12379_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 01 02

foot

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 22 23

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 18

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 06 07 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 03 04 05 06 07 16 17 18 19

trigeminal v nerve

strong

regional

strong expression: see section 07 14 15

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 11 15 16

cervical ganglion

strong

regional

strong expression: see section 08 16 17

thoracic ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 13 14 15 16 17 18

neural retina

strong

regional

strong expression: see section 01 02 20 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

tongue

strong

spotted

strong expression: see section 13 moderate expression: see section 10

stomach

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08

midgut

strong

spotted

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

lower jaw molar

strong

regional

strong expression: see section 04 05 17 18

upper jaw molar

strong

regional

strong expression: see section 04 05 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9420
Entity Detected:	Pcdha7, protocadherin alpha 7 ( MGI:1298369)
Sequence:	sense strand is shown >T9420 TTGGGCTTCACTCTTCGGTTGCTCCACAATGGCCAGCATCTCGGGACTGGAATGAAGAAATAAAAAACCT CCAGAATTTGGGATAACTGTCGCGAATGAGAATAAAACGTCAGCAATTTGAAATGGTAAACCTGAGAGGA TACAACTGGAAGAGCCAACAATTACTCCTGTTCCTGATAATTGTGGCAGCCTGGGAGGCAGGGAGCGGCC AGCTCCACTACTCCGTCCCAGAGGAAGCCAAACACGGCACCTTCGTGGGCCGCATCGCGCAGGACCTGGG GCTGGAGCTGACGGAGCTGGTGCCCCGCCTGTTCAGGGTGGCGTCCAAGGACCGCGGGGACCTTCTGGAG GTAAATCTTCAGAATGGCATTTTGTTTGTGAATTCTCGCATCGACCGGGAGGAACTGTGCGGGCGGAGCG CGGAGTGTAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGTTTTCCACGTGGAGGTGGAGGT GAAGGACATTAATGACAACCCTCCCATGTTTCCAGCGACACAAAAGGCTCTGTTTATCCTGGAGTCCAGA CTGCTTGACTCGCGGTTTCCACTAGAGGGCGCGTCAGATGCAGATGTCGGTTCCAATGCTCTGCTGACTT ACAGACTGAGCACCAATGAACATTTCTCTCTGGATGTACCGCCCAACCACGAGCAGGTGAAACCTCTTGG GCTGGTTTTGCGGAAACCTTTAGACAGAGAAGAAGCTGCAGAGATTCGCTTATTGCTCACGGCCACCGAC GGAGGCAAACCGGAGCTGACAGGCACCGTTCAATTACTCATCACCGTGCTGGATGTCAATGACAATGCTC CAGTTTTTGACAGATCTCTGTATACTGTGAAATTGCCAGAAAACGTTCCAAATGGAACATTGGTAATCAA AGTCAATGCCTCAGATTTGGACGAAGGCGTGAATGGGGACGTTATGTACTCATTTTCTAGTGATGTTTCT TCAGATATAAAATCCAAGTTCCACATGGACACTGTGAGTGGAGAGATTACAGTTATAGGAATTATTGATT TCGAAGAGAGTAAAGCTTACAAGATTCCATTGGAGGCACGTGATAAAGGGTTCCCACAACTGCCTGGACA CTGTACCATTCTTGTGGAAGTTGTAGATGCTAATGACAACGCTCCACAGTTGACGGTTAGTTCCTTGTCT CTCCCAGTATCCGAGGACAGTCAACCTGGAAGAGTCGTCACTTTAATTAGCGTATTTGACCGTGATTCCG GTGCCAATGGGCAGGTGACCTGCTCCCTGACTCCTCATATCCCCTTCAAGCTGGTGTCCACATTCAAAAA TTACTATTCGCTCGTGCTGGACAGTGCTCTGGACCGAGAGACCATCGCTAACTATGATGTGATTGTGACC GCTCGCGATGGCGGTTCGCCCTCCCTGTGGGCCACGGCCAGCGTGTCCGTGGAGGTGGCTGATGTGAACG ACAATGCTCCACTGTTTGCGCAGCCTGAATACACGGTGTTCGTGAAGGAGAACAACCCGCCTGGTGCGCA CATCTTTACAGTGTCAGCGATGGATGCTGATGCGCAGGAGAATGCGCTGGTGTCCTACTCGCTGGTGGAA CGGAGGGTGGGCGAGCGCTTGCTGTCGAGCTATGTGTCTGTGCACGCGGAGAGCGGCAAGGTGTTCGCTC TGCAGCCTCTGGACCATGAGGAGCTGGAACTGCTGCAGTTCCAGGTGAGCGCGCGGGATGCTGGTGTGCC TGCCCTGGGCAGCAATGTGACTCTGCAGGTGTTTGTACTGGACGAGAACGACAATGCACCTACCCTGCTG GGGCCTTTGGCTAACGGGGTTGGTGGCACAATGAATGAGGTAGTATCAAGAGCTTTGGTGCCTGGTCAGG TTGTGGCGAAAGTCCGGGCCGTAGATGAGGACTCTGGATACAATGCGTGGTTGTCTTTTGAGCTGCAGCC AGTTGCGGGTGGAGTTCGTAGTCCCTTCCGTGTAGGCCTGTACACAGGAGAAATCAGCACGACACGTGCT CTGGAGGAGACAGATGCCTTGCGGCAGTGCTTGTTAGTGTTGGTGAAAGATCACGGAGAACCTTCTCTGA CCGCCACAGCCACGGTGTTGCTTTCTCTGGTAGACAGCGGCCAAACACAGAAGGTCTCATCTAAGGTGTC AGCTGGAGCCTCCAGAGTGGATCAGAGGCTGGTGGATGTCAACGTGTACCTGATCATCGCCATCTGCGCG GTGTCCAGCCTGTTGGTGCTCACGCTACTGCTGTACACAGCGCTGCGCTGTTCGGCGACGCCCACCGATG GAGCCTGTGCTCCAGGGAAGCCCATGCTGGTGTGCTCCAGCGCGGTGGGGAGCTGGTCATACTCGCAGCA GAGGCGACAGAGAGTGTGCTCTGGAGAAGGCCCACCCAAGACCGACCTCATGGCCTTCAGCCCCAGCCTA CCTCAGGGTCCCAGCTCTACAGATAACCCCCGGCAGCCCAACCCTGACTGGCGCTACTCTGCCTCGCTAA GAGCAGGCATGCACAGCTCTGTGCACCTGGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGA TCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAACCTGAGGCAGGAGAGGTGTCCCCTCCGGTGGGC GCCGGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAGTCCGGTCCCGGTG AGTTGCCAGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCGGCAGGAGCCTGCTAACAT CTCCATCCGGCAGGAGCCTGCTAACAACCAAATTGACAAAAGCGATTTTATAACCTTCGGCAAAAAGGAG GAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAAGGGAACAGCA CGACGGACAACAGTGACCAGTGAGGCCACCAAATGGAAACAAGCCACTTAGCCAGTTTTTGTAATAATGG CAAATCTCTCCCATGTAGCAACTCCCCGCTCCTTTCTCCTATGACATGAGCCCTCAGAAATCTGCAGAAA GTTCCCTGTGTCTGTCTTGATCGCATTTAACAGGTTTTGTCTTAAAAAGCTTTCCTAAGTCTGGTGTTAA CTCTCTCTCTCTCCACTCTGGCTTGTTTTCAGAACCTAAAAAGCAGACCCAGGTTTCCTTTCTCCCCCGC CGCAAAGGAGAAGCTTCCCAGCCCCGCCAGTGAGAGTTGGACTCTCTGCCCTGTGCTTCAAGCATCCTGT CTTGATGATATTTGCAGGGCAGGCTGAAAGGTATTCAGGTTGAGCAGTTGGGTGTTTGTGGTCACTGGGT ATGTGTGGCTACCAAGAGTGTTGGAGAGCCTGGTATTGGCTGGGATGGTCCAGATTAGACTAGTTAACAC AAGGAGGGCTGGGGCTCAAAGGCACATCAACACCGGGAGTCTTTCATCTGGAGGGGGAAAATGTGAAACT TACAAGGACCAGACTTTCTCAATCTCTCAACTAGACATATGATGGCCATCCTCTAACAGACAAAACCATC CCCACCGGCAAAGCTTTAGGAGCCCCTCAAGTGTGCTGGCTATAACATCACTGTATTCAAAACCTGCAGT ATGCACACGAGCCAGCAGTTCAAGCGTTTAACAAGAGGGTCGGCCAGGGCAACAGAAGCAGATCTGATGT GTTTCCTGTACACGTCCTTGTGCTCACGCTATTAAAAATTCTTTTGCACACAATGTTTATGAAAAGGTCT CATCCTTTTCCAACAACACATATGCAAAAGCAAAAGAAAAACCCAAGACCTCACTTTATGCTGTTTGTTG TTTGATAGATTTATTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30356469 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30356469 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12380 same embryo
	EurExpress:euxassay_008269 same experiment
	MGI:4827082 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source