EMAGE mouse gene expression spatial database

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EMAGE:12380

2310007B03Rik RIKEN cDNA 2310007B03 gene ( MGI:1919124)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008245. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008245_01	euxassay_008245_02	euxassay_008245_03	euxassay_008245_04

euxassay_008245_05	euxassay_008245_06	euxassay_008245_07	euxassay_008245_08	euxassay_008245_09

euxassay_008245_10	euxassay_008245_11	euxassay_008245_12	euxassay_008245_13	euxassay_008245_14

euxassay_008245_15	euxassay_008245_16	euxassay_008245_17	euxassay_008245_18	euxassay_008245_19

euxassay_008245_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12380_wholemount_strong.wlz

12380_wholemount_moderate.wlz

12380_wholemount_weak.wlz

12380_wholemount_possible.wlz

12380_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12380_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9986
Entity Detected:	2310007B03Rik, RIKEN cDNA 2310007B03 gene ( MGI:1919124)
Sequence:	sense strand is shown >T9986 AGTCTGGACTTGCACAACTCTGAGCAGGGAGGCGGCACCGGGCAGGGCACCCACTGGCAGGTACTGACTC ACCCCACTTCAAAGGCCTTGGCCTTGGTTCAGCTATTTAACCTGCTGCGAGAAGCCTGTCACTCTGGAAC TCTGCCTGTGGAGAGCTGAGCCCTGCCTGCAGACCTGCTCCTCACCTGGGCAGGTACCTCTTCCTCCGAG CCAGTCTACACAAGCTCGAGAGCGGTCCCCAAGAGCCAGCGGGGCCCTGCTGCCTGCTGCTCGGCTCAGG GATGCCTGGCTCAGAGATGCCTGCTCCCTGTGTCTTTGCTCCAGACATGGCGGAGCAGGTGCCCCTGTGG CATCACTACCTGTTGGCCATCCAGTCACGGGAGTCACCCCGTGTCCAGGACTACCAGAGAGCGGAGAATA TCTTGCTTACGGTGCTGGAACGCGTCCATGCCTTGGACTCCCGCTTCATTGTTGACTATTCTAGAGACCT GGAGGCCTTCCAGTTTGCCCTGCGTTCCTCAGAGGACCCACTGGATGTGGAGGTGCTCCTGGGGGTGGAT TCGGAGGCTCTCCTGATCGAGGAGTCAGAAGCCACTGAGCCAGGGGATGGCCCTGCAATCTGTCGCCTGG GTGTCCTCAAGGAGGCATCTGGCCTGGAGCCATGGATGACTGCTGACATCTTCAGTGTCTCCTCAGAGGA CAGAGACAAGTGCTGTGGCCACCTTGTACCCAGTAAAGTCCTGTGTGTCCTCAAAGATCTTCTGGTGGCT GCCATTGTACATTGCAAGCACCACAGGCTCATTCCACCAGGTTCACTGAATGCTGCTAATCTGAAGGAAG GGCAGATGCGCCTGTCTCTGCTGGTGTCCAGTGGCTGGAGGAAGATCCGCTTCAACGTTGTGCCCGTGGT GAGGAAGAAGCACCGGGTGCCTGCCTTGGAGGGGGCCCAGTTGAAGCTTGGATTTCCTGAGGGCATCTTG AGAAGAATTGCCAGCCACGGGGTGGACCTGGTACCAGCTAATGCCCAGCACTGGAGGATCTCCACTGGCT ACCTGCTCAGTCGCCTGCTTGATGCACTAGGCTCCCTCCCTGGCCACCGCCTGGACAGCCTCTCCATTCT CGACCGCGTAAACCTAGAAAGTTGGCAAGGTGGCAGCCAGAACCATGGCCTAACTTTTGACCACCTGAAG ACAGTGCTGCTGTGGGCCTCTACGCTGTTCCCAGCACCAGAGGACTGGGCTGACCTGCAGGGCTCAGTGT ACCGCCAGCTGGTGGTGCTGCTCTGCTGCCTGGCTACCAGGAAGCTGCCCCACTTCCTGTACCCGGAGCA CAACTTGCTGCAGGATGGTGGCCTGGACCTTGGCGCCATCTACCAACGAGTAGAACACTTCGCCAGCCAG CCCGAAGAGTCCTTACGAATCCATGTCACCCACCTGGGCCCCAGCCGTCCACCACGCATCGACAATGGTG TCAAAGCGCTACTGCAGCTCCCAGCCAGCGACCCTACCTACTGGGCCACCGCCTACTTTGACTTTCTGCT AGATAAGTTCCAGGTCTTCAACATCCAGGATAAGGACAGAATCTCAGCCATGCAGAACATCTTCCAGAAG ACCAAGACCATGGGGAGTGAGAACACCTGAGCTGGGCTGGGAGATCCAGTGTCTACCTGTGGAGCTCCCT AGAACTTTTGGAAAAGGAATGGGGAACAGGGATGTCTCTTTGAGTGACTGGTGGAGGGACTTAGTCCCTA AGGACTCCTGTTCAAGAGATCACTGTGAGAGTCTAAGTATCCTGCCCTGGGGACTTGAGGCAGCTGCCAT GTTCCAGTCTGAGTTTGAAGTCCATGGGTATATGCTTCCCAGAAACCCACCGATCCATCCATTTGATAGC CGAACAGGTACAGCCACGAGACAACACGGGTGCTTGCTCTGCTATTCTCCACACTACTCCTTGGGGTTGA AATACTATGTGTTCAAGGTTGAACACATTAATGCTCCAGTGTACCCCATGGAGAGGGCCCCCCTACTTTC TGTATCTCTCAATGCAAATATAGACTCCTAAAACTATGAAAGGCTCATGTGCAAAGGATGGTTTCATTTC AAACCTTCTCTACTCTCGGGTAGAGAGTGTTCTCCTGGACAGCCATCTCTACTCTGCAGAAGAGGGAATT CCATAGCTCCCCTTCCCCCATACCAGTCTTACCTGCTTCTCTGTCACTGTGTTCTCTGTTCCCAGAGTTC TGTGATTCCCCATATGCACTTATTTAATCTTCTCATTAAATGCCTTAAGCAGTAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30315016 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30315016 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12379 same embryo
	EurExpress:euxassay_008245 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source