EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12382

Mr1 major histocompatibility complex, class I-related ( MGI:1195463)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007120. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007120_01	euxassay_007120_02	euxassay_007120_03	euxassay_007120_04

euxassay_007120_05	euxassay_007120_06	euxassay_007120_07	euxassay_007120_08	euxassay_007120_09

euxassay_007120_10	euxassay_007120_11	euxassay_007120_12	euxassay_007120_13	euxassay_007120_14

euxassay_007120_15	euxassay_007120_16	euxassay_007120_17	euxassay_007120_18	euxassay_007120_19

euxassay_007120_20	euxassay_007120_21	euxassay_007120_22	euxassay_007120_23	euxassay_007120_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12382_wholemount_strong.wlz

12382_wholemount_moderate.wlz

12382_wholemount_weak.wlz

12382_wholemount_possible.wlz

12382_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12382_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7112
Entity Detected:	Mr1, major histocompatibility complex, class I-related ( MGI:1195463)
Sequence:	sense strand is shown >T7112 CCACGCGTCCGCCCACGCGTCCGCGGTAGAGTACAAGTTCTCAAAAGATTCATCAGGGGTCTGTCACTGT CTGAGGTTAAAAAAAAAAAAATCAGAAAAGCAAAGGACTTCAGCGTGGGTTGATGATGCTCCTGTTACCT CTGCTCGCTGTATTCTTGGTGAAGCGAAGCCATACTCGGACCCACTCGCTGAGATATTTTCGTCTGGCTG TTTCCGATCCTGGTCCCGTCGTCCCTGAATTTATCTCTGTTGGGTATGTGGACTCACACCCTATCACTAC ATACGACAGTGTCACTCGACAGAAGGAGCCGAAAGCTCCATGGATGGCAGAGAACCTGGCACCTGATCAC TGGGAGAGGTACACTCAGCTGCTAAGGGGCTGGCAGCAGACATTCAAGGCGGAGCTGAGGCACCTACAGA GGCACTACAACCACTCAGGGCTTCACACCTACCAGAGAATGATTGGCTGTGAGTTGCTAGAAGATGGCAG CACCACAGGGTTTCTCCAGTATGCATATGATGGACAAGATTTCATCATCTTCAATAAAGACACCCTCTCC TGGTTGGCCATGGATTATGTGGCTCACATCACCAAGCAAGCATGGGAGGCCAACCTGCATGAGTTGCAAT ACCAAAAGAACTGGCTGGAAGAAGAATGCATTGCCTGGCTAAAGAGGTTCTTGGAATATGGAAGAGATAC CCTAGAAAGAACAGAGCATCCAGTGGTAAGAACAACTCGAAAGGAAACTTTTCCAGGGATTACAACTTTC TTCTGCAGAGCTCATGGCTTCTACCCACCAGAAATTTCCATGACATGGATGAAAAATGGGGAAGAAATTG CCCAAGAAGTGGATTACGGAGGGGTACTTCCCAGCGGGGATGGAACCTATCAAACGTGGCTGTCAGTTAA TCTGGATCCTCAGAGCAATGATGTTTATTCTTGTCATGTGGAGCACTGTGGTCGCCAAATGGTTCTGGAG GCCCCTCGGGAATCAGGGGACATCCTTCGAGTGAGCACGATCTCTGGAACCACAATTCTCATCATCGCCC TGGCTGGAGTTGGTGTTCTGATCTGGAGAAGGTCACAAGAACTAAAAGAAGTCATGTACCAACCCACCCA AGTGAATGAGGGAAGCTCTCCCTCTTAGGAGCCACAATCTCTGCTCTCAGGCATCCGCATGTCTTGGCCA TACTCCTGATGCAACCGGGGACTGTAAAACTGAGCACTTGTGACAGGAAGATAGGAGCTACAAATTTTTC TTCATTCTTTGGCTCCAGAAGAGCTGTCAACTTTTAGGCTCTTGATAGACTCCTTTATCACAGTCAACTT TAAATATAGTTTGTCTCATGACCCAATACTTCCCAATATTGTAGTTCTCAATAGTGATTTACAAGTAGAT CACAGAGTATCAGAGCCGCAAGCAACTTTCTCACCTAAGCAGGAGATGTCTTCTAAAATGTCATAGACTT GAGCATTAGCCTTCACTCAACATTATGTGCAAGTTCCCCCATTCTACTACAAAACAGCCTTTCTTGTTCA TTTTCTCTCCTGAGAATGTTTACCATGTAAGAACTTTGACCACTATGGCTCTCATGTTTGAGAATCTATA AGTAGGGTTGAGCCAACCCATGAAAATAGCCAGCAAGTTTCTACAGCAGCCTTTGAGTGGACGAGTCACT ATATACATATCATGGCCCAAGGGGAGGCAGAAGTATCCAGTTCATGGTGGCTCTGTCAGAAAGCAAGGAA TATTGCATGTTTGTCTGGCTACATTTATGGCAATGACTTCCTTTGCTTTTACTGTTTCTGTGAGAAATTA GAACAATAATTTATCAAGCTGAGTGTGATGGGAATCTTAGAAGCCCAGTACTCAGGAAGCTGAAGCAGGT AGATTTTACATTTGGGGCTAGCTTGAGCTACACAATGAAACCCTATCTGAAATAAACCAAAACAACAACA ACAGCAACACACTTAACAGAACTAAAACAATCATTACCAGACATATTGTCCCCTCCCATGGAACGGCAGA CCTAGACTCACTTGCAATATTTCAACATGAAATAATAGTGTTTGGCTGGAGATGTCCAGGTGGGTGAAGA TGTCTGTTGCCAAGCATGTCCACCTGAGGTCAGACACCCTGACCCACACACACAGTGAAAGAAAAAAACT GACTCCTGAAAGTCGCCCTCTGACCTCTCCATGAGTGCTAAACTAAGGTTTTTATTTTTGTTTTTGTTTT AATCTGACAAAATGTGTTCATATGCTTATGTCTCAAAACGAGTTCTCTTTACTCTGGTATTGGAGACTGA ACCAGTACAGAAAAGACAGGAGTTAGAATTGAACCTTTGGGGGTTTAGGGTGTAGCTCAGTGGCAGAGAA TTTTCTTAGCATGTATGAGGCCCTGAGGTCAGTCTTCAGCACTTTGAACACACACACATACACACACCAC CACCACCACCACCACTAATAACAAACAAAAACTAAAACTTTGGATCTTTCTTCTGTAAGTGGTTTTAAAT TAAATTGTTTCCTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:4017492 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4017492 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12386 same embryo
	EMAGE:12384 same embryo
	EMAGE:12381 same embryo
	EMAGE:12383 same embryo
	EMAGE:12385 same embryo
	EurExpress:euxassay_007120 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source