EMAGE mouse gene expression spatial database

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EMAGE:12383

Api5 apoptosis inhibitor 5 ( MGI:1888993)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007141. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007141_01	euxassay_007141_02	euxassay_007141_03	euxassay_007141_04

euxassay_007141_05	euxassay_007141_06	euxassay_007141_07	euxassay_007141_08	euxassay_007141_09

euxassay_007141_10	euxassay_007141_11	euxassay_007141_12	euxassay_007141_13	euxassay_007141_14

euxassay_007141_15	euxassay_007141_16	euxassay_007141_17	euxassay_007141_18	euxassay_007141_19

euxassay_007141_20	euxassay_007141_21	euxassay_007141_22	euxassay_007141_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12383_wholemount_strong.wlz

12383_wholemount_moderate.wlz

12383_wholemount_weak.wlz

12383_wholemount_possible.wlz

12383_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12383_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6588
Entity Detected:	Api5, apoptosis inhibitor 5 ( MGI:1888993)
Sequence:	sense strand is shown >T6588 GCCCGGCGGCGGCTCCCGGCGTACTAGTGCGGTCGACGGCTCAGATAAGTTCCGTGGAGGCGGCGGTGGC GCCGACGAGGTGTTGGGCGACGGGAGCGGGCCCGGAACTTGGCGCTCAGCATGCCGACGGTGGAGGAGCT GTACCGCAACTACGGCATCCTCGCCGATGCCACGGAGCAAGTGGGCCAGCATAAAGATGCCTACCAAGTG ATACTGGATGGCGTGAAAGGCGGCACCAAGGAAAAGCGATTAGCAGCTCAGTTTATACCAAAATTCTTTA AGCATTTTCCAGAGCTGGCTGATTCTGCTATCAATGCACAGTTAGACCTCTGTGAGGATGAAGATGTGTC AATACGACGTCAAGCAATTAAAGAGCTGCCTCAGTTTGCCACAGGAGAAAACCTTCCTAGAGTAGCAGAT ATACTGACCCAGCTTCTGCAGACAGATGACTCTGCAGAATTTAACCTGGTGAACAATGCTCTGCTAAGTA TATTTAAGATGGATGCAAAAGGAACGTTAGGTGGCTTGTTTAGCCAGATTCTTCAAGGAGAAGACATTGT TAGAGAACGAGCAATTAAATTCCTGTCCACAAAACTCAAGACGCTCCCAGATGAGGTGTTGACTAAGGAA GTGGAGGAACTCATACTCACTGAGTCCAAGAAGGTCCTAGAAGACGTGACTGGTGAAGAGTTTGTTTTGT TCATGAAGATACTGTCTGGGTTGAAAAGCTTACAGACAGTGAGTGGACGACAGCAGCTGGTGGAGCTGGT GGCGGAACAGGCTGACCTGGAGCAGGCCTTCAGCCCCTCAGACCCCGACTGTGTGGACAGACTACTGCAG TGCACGCGGCAGGCTGTGCCTCTCTTCTCCAAAAATGTGCATTCTACAAGATTTGTGACGTATTTCTGTG AGCAAGTTCTCCCTAACCTCAGCACCCTGACCACCCCAGTGGAAGGCCTCGATATACAGCTGGAGGTATT AAAGTTGTTGGCAGAGATGAGTTCATTTTGTGGTGACATGGAAAAACTAGAAACAAATTTAAGAAAACTG TTTGATAAGTTATTGGAGTATATGCCTCTCCCTCCAGAAGAAGCAGAAAATGGGGAGAATGCTGGTAATG AGGAGCCCAAGCTGCAGTTCAGTTATGTGGAGTGCTTATTGTACAGTTTTCATCAGTTGGGGCGAAAACT TCCAGACTTCTTAACAGCCAAACTGAATGCAGAAAAACTCAAGGATTTCAAAATTAGGTTGCAGTACTTT GCACGGGGCCTGCAGGTTTATATCAGACAACTTCGCTTGGCTCTCCAGGGTAAAACAGGCGAGGCCTTAA AAACAGAGGAGAACAAGATAAAAGTTGTTGCACTGAAAATAACAAACAATATCAATGTTTTAATCAAGGA TCTCTTCCACATTCCTCCTTCTTATAAGAGCACAGTAACATTGTCCTGGAAACCTGTGCAGAAAGTTGAG ATTGGGCAAAAGAGAACCAGTGAAGATACAAGTTCAGGTTCACCACCTAAGAAGTCTCCAGGAGGACCAA AAAGAGATGCCAGACAGATTTATAATCCTCCGAGTGGAAAGTACAGCAGCAATCTGAGCAACTTTAATTA TGAGAGGAGCCTTCAGGGGAAATAGAGGTGGCCGAGGTTGGGGAACACGAGGAAATCGAAGTCGAGGAAG ACTCTACTGATTGTGACGTCACATTCTTCAGCATTGTCATGAGATTAATATACTTAAATCTACTACTCAT TGGATTGCCGGGGATGTCCCTTTAAAAGGACTGCTGCCTTCAGCTGAAAATGTAATGTTCTTTCTACCTT TGTATGTATGATCTACTTTTGTAAAAGACCATGGTTGTGTCCAAGGTAAAACCACAACAATATTTTTGGA TGCTTTGTCTGCAGCCTTGACTTGTTTTCACAATATCCTCACTATCCAGACTTCATCTTGTGAATCTTGC TTTTACACATCTTGATGGTTTGTCACTGAGATCTGACTCTATTGGAAAAGTCAGTCTAGCCTGTGATCTG GTGAAGATCATCACTCTTGAAACGTTACTGTTCTCCTCCCCATTCCGTTAGGTTTCTGCCCAACACATGG AGAAGAGTTAAGAGCAGTCTTAACCTTCTGCTTTCATTGTTTTCTAAGGAAGCTGCTAGGCAGTGTTGTC GCATTCCTGCCAAGTCATAGAAATGAAGCACCAGCTTCTCACAGTGTACAGGCGATGCCAGCCTTTTAGA AAGAACTTGCTTTGCATTGCCCCTTCAGTCTTCTCCATATTTTGGGATACAGCTTTGAACACAGGGTCTT ATTATTTGAATAGGAGTACATGTGCATAATATACGTACAGACATAAGCATATGTTGTGTGTGTATATATG CATGCTATGGAACTTGAGTGTACAACCAATCAATACATTAAATTCTCGGAAGGGGTCCTCTGACATAGCG ATTATCCCTTTGAGGCTGAATCCATTACTGACTTGGTATCTAGGTTTTCACTCCAGTAGGGAGGAATTTC AGTCAGCTGAACTTACAGATTATTATTGTTGTTTAAAACTAAAAAACAAAGGCTGTTTTTACAGAGACAA ATTTAAAGACCCTTGTTGGTGAAATACAGCCAGATCTGGTGTACATACAGGTTCCCACAGGATCTGCTAG TGTAATGTAGGATTTGGAGACTTAATAACCAGGGCTACCCAGGAGTGTGACTTGGTGACATAGTACCATA AAAGTTGCTCACTCCGCTTGCTTTTGCCACTTTCAAATTTTAACTTCTCAGGTTATTAATCAGATTATTG TGTAAGTTAGCCAATAGTCTTTAGATTAAGGCAACAGACGGGAGGTTCGTGGAGTGTCTCATGTTGGGCA TTTTTAGTAGCCCAGACTCTGTTCTTCATTTGAATGTTTCACACATTTTTGTTCACAGTTAATCTTCCAA GTTTACTATTCAAATCAGAAATTCAGATGACATTTCTAGTGGTTTGCTGTTTTGGTTTTTTATGTTTTTT GGTTTTTTTGAGGTTTCATTTCTTACACAGGTGTCTTCATCACCATCACTTCTACACTGGGGGAAAAACA ATCTCCTTTGTGAGAATCACTGCACGTATTTATGGCGAAAATATTTCTGAAAGTCTAGAGTGATACAAGT GAGCACAAGAAGTTGGTCAGCTTGCCTATGGAGTGCTGGCAATAAACTCTGAACATTCCACAAGCCTGAG CTGAACCTAGGCTCCCTTGGAAGCTGAACAGACATAGGAACATGGGATTGCCAGCTGAATCCTGTGACCA TCTAGTTATGGACCTCAGAGATAGATCAGCATGGCCTAAAGCCATTTCAAGTACAAAAATGAATTGGACT ATAGCAACATAAATTTGAACACAGTAACTTTTCCTCTTTGCCTGAGGGCATTTGTAAACTCGGCAGAAGT AACTTGACCTCATGTCTACTGCAGAGCTCACTGCATTCACCCCTCCTCATCCTTTGCTTCCTTCCCCTTG CCTAGTCAGTAGTTCATAACTTAGTGTTCCTTTTGCTTCAGAAATCTGAAAGAAAATCTTCATGCCATGA ATCTTTTTCTTCCGCAAGGACACCTGTGTCTCCCTTGTTTAAATAAATGTAAATGCTCCCTTATGCTTTT GAAATAAATTTCCTTTTGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3594358 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3594358 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12386 same embryo
	EMAGE:12384 same embryo
	EMAGE:12382 same embryo
	EMAGE:12381 same embryo
	EMAGE:12385 same embryo
	EurExpress:euxassay_007141 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source