EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12385

Becn1 beclin 1, autophagy related ( MGI:1891828)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007144. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007144_01	euxassay_007144_02	euxassay_007144_03	euxassay_007144_04

euxassay_007144_05	euxassay_007144_06	euxassay_007144_07	euxassay_007144_08	euxassay_007144_09

euxassay_007144_10	euxassay_007144_11	euxassay_007144_12	euxassay_007144_13	euxassay_007144_14

euxassay_007144_15	euxassay_007144_16	euxassay_007144_17	euxassay_007144_18	euxassay_007144_19

euxassay_007144_20	euxassay_007144_21	euxassay_007144_22	euxassay_007144_23	euxassay_007144_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12385_wholemount_strong.wlz

12385_wholemount_moderate.wlz

12385_wholemount_weak.wlz

12385_wholemount_possible.wlz

12385_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12385_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5745
Entity Detected:	Becn1, beclin 1, autophagy related ( MGI:1891828)
Sequence:	sense strand is shown >T5745 GGGAAGTAGCTGAAGACCGGGCGATGGGAACTCTGGAGGTCTCGCTCCGGGGCGGACCCGAGGCCACCGT GCCCCCGCGGTAGAACGAGCCTGGGTGGCGGGCTCCGGGCTCCTGAGGCATGGAGGGGTCTAAGGCGTCC AGCAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGCTGTAGCCAGCCTCTGAAACTGGACACGAGCTTCA AGATCCTGGACCGGGTCACCATCCAGGAACTCACAGCTCCATTACTTACCACAGCCCAGGCGAAACCAGG AGAGACCCAGGAGGAAGAGGCTAACTCAGGAGAGGAGCCATTTATTGAAACTCGCCAGGATGGTGTCTCT CGAAGATTCATCCCCCCAGCCAGGATGATGTCTACAGAAAGTGCTAATAGCTTCACTCTGATCGGGGAGG CATCTGATGGTGGCACCATGGAGAACCTCAGCCGGAGACTCAAGGTCACTGGAGACCTGTTTGACATCAT GTCGGGCCAGACCGATGTGGATCACCCGCTGTGTGAGGAATGCACAGACACTCTTTTAGACCAGCTGGAC ACTCAGCTCAATGTCACTGAGAATGAATGTCAGAACTACAAACGCTGTTTGGAGATCCTAGAGCAGATGA ATGAGGATGACAGTGAGCAGCTGCAGAGGGAGCTGAAGGAGCTGGCCTTGGAGGAGGAGAGGCTGATCCA GGAGCTGGAAGATGTGGAAAAGAACCGCAAGGTGGTGGCAGAGAACCTGGAGAAGGTCCAGGCTGAGGCG GAGAGATTGGACCAGGAGGAAGCTCAGTACCAGCGGGAGTATAGTGAGTTTAAAAGGCAGCAGCTGGAGT TGGATGACGAACTCAAGAGTGTGGAGAACCAGGTGCGCTACGCCCAGATCCAGCTGGACAAGCTCAAGAA AACCAATGTCTTCAATGCCACCTTCCACATCTGGCACAGCGGACAGTTTGGCACAATCAATAATTTCAGA CTGGGTCGCTTGCCCAGTGTTCCTGTGGAGTGGAATGAAATCAATGCTGCCTGGGGCCAGACAGTGCTGC TGCTCCATGCTTTGGCCAATAAGATGGGTCTGAAGTTTCAGAGGTACCGACTTGTTCCCTATGGAAATCA TTCCTATCTGGAGTCTCTGACAGACAAATCTAAGGAGTTGCCGTTATACTGTTCTGGGGGTTTGCGGTTT TTCTGGGACAACAAGTTTGACCATGCAATGGTAGCTTTTCTGGACTGTGTGCAGCAGTTCAAAGAAGAGG TGGAAAAAGGAGAGACTCGATTTTGTCTTCCGTACAGGATGGACGTGGAGAAAGGCAAGATTGAAGACAC TGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAACTCGGAGGAGCAGTGGACAAAAGCGCTC AAGTTCATGCTGACCAATCTCAAGTGGGGTCTTGCCTGGGTGTCCTCACAGTTCTATAACAAGTGACTTG CTCCTTAGGGGATGTTTGCCTTTAAGGTTTTATACTTTGTTTGGTTTGGAAAGATGCTTTAAATTAAATT TGGGTAATATTAAACCACATGTTTACAATACCAAAATCCACAAAAGCTACTTTATTTTCAAATATGACAG ATAGTTTCCAGAGTACGCCATGTATAGCAAAGAACCCTGCCATAGTTTTGACTCAGCCCCATGCATCCTT TCCCTCTTTCCTGAAAACAACTAATTTAAATTTGCTTTGTTTTCTTTTTTAAGTTGAATTGACGTTAATG TGTTTTCACTGGATTTTATCTCTCTCAACTTCCTGCACTTAAAATTTGAAACAGCAAAGGTTTGAGATGA GATGCTTGTGGCACACAGTTGGGTGATGTGGGGAAAGGACACCGGGTCAGGAGTTGCAAGTTTAACTCCG TCCTCACTTGTAGCATTGAATGCCTCCTGTGCTGTCTAGTGGGACTACAGAATGCTGTTTGATACTGTGT GCGACGTGGAGAGATTTAATTATTTGTAATAAAGGATTTGCTATGGTCTATTAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:2650059 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2650059 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12386 same embryo
	EMAGE:12384 same embryo
	EMAGE:12382 same embryo
	EMAGE:12381 same embryo
	EMAGE:12383 same embryo
	EurExpress:euxassay_007144 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source