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EMAGE:1239

Nefm neurofilament, medium polypeptide ( MGI:97314)
TS18 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:1239
Fig 10a Caton et al, 2000 [PMID:10725250] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: V, trigeminal; VII, facial; IX, glossopharyngeal; X/XI, vagus; cranial accessory; XII, hypoglossal nerves.
Expression Pattern Description
Spatial Annotation:
EMAGE:1239Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1239_wholemount_strong_3D_1.wlz
1239_wholemount_moderate_3D_1.wlz
1239_wholemount_weak_3D_1.wlz
1239_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1239_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cranial ganglion
detected detected
homogeneousExpression is in cranial nerves
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1330766
Entity Detected:Nefm, neurofilament, medium polypeptide ( MGI:97314)
Notes:The anti-Nef3 antibody used in this study by Caton et al, 2000 [PMID:10725250] is described as "anti-NF160 antibody (N-5264; Sigma)". Reacts specifically with the neurofilament polypeptide with apparent molecular weights of 160 kDa and does not cross-react with other intermediate filament proteins.
Antibody Type:monoclonal
Raised In:mouse
Supplier:Sigma
Catalogue Number:N-5264
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:Dent's fixative (1:4 DMSO/methanol)
General Information
Authors:Caton et al, 2000 [PMID:10725250] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10725250] Caton A, Hacker A, Naeem A, Livet J, Maina F, Bladt F, Klein R, Birchmeier C, Guthrie S 2000 The branchial arches and HGF are growth-promoting and chemoattractant for cranial motor axons. Development (127):1751-66
Links:MGI:1354358 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI