EMAGE mouse gene expression spatial database

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EMAGE:12391

Spock1 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 ( MGI:105371)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008541. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008541_01	euxassay_008541_02	euxassay_008541_03	euxassay_008541_04

euxassay_008541_05	euxassay_008541_06	euxassay_008541_07	euxassay_008541_08	euxassay_008541_09

euxassay_008541_10	euxassay_008541_11	euxassay_008541_12	euxassay_008541_13	euxassay_008541_14

euxassay_008541_15	euxassay_008541_16	euxassay_008541_17	euxassay_008541_18	euxassay_008541_19

euxassay_008541_20	euxassay_008541_21	euxassay_008541_22	euxassay_008541_23	euxassay_008541_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12391_wholemount_strong.wlz

12391_wholemount_moderate.wlz

12391_wholemount_weak.wlz

12391_wholemount_possible.wlz

12391_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12391_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 14 15 16 weak expression: see section 13

diencephalon

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vibrissa

moderate

regional

moderate expression: see section 07 23 weak expression: see section 06 08 09 22 24

cerebral cortex mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 19 20 24 moderate expression: see section 17 18 21 22 23

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 20 24 moderate expression: see section 18 19

olfactory cortex mantle layer

strong

regional

strong expression: see section 11 12 13 14 16 moderate expression: see section 17 18 19

hindbrain

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 04 05 21 23 weak expression: see section 22

midbrain

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 05 21

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 moderate expression: see section 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 09 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 19 20 24 moderate expression: see section 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 10 18 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 18 19 20

spinal cord

strong

homogeneous

strong expression: see section 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 11 moderate expression: see section 18

cervical ganglion

strong

regional

strong expression: see section 10 moderate expression: see section 18

thoracic ganglion

strong

regional

strong expression: see section 16

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 14 18 19

neural retina

strong

regional

strong expression: see section 01 02 03 04 moderate expression: see section 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 12 13 14 16 17 18 19 20 moderate expression: see section 09 10 weak expression: see section 21

vomeronasal organ

strong

regional

strong expression: see section 13 16 17

lower jaw incisor

strong

regional

strong expression: see section 13

lower jaw molar

strong

regional

strong expression: see section 08 09 20 21 moderate expression: see section 07 weak expression: see section 19

upper jaw incisor

strong

regional

strong expression: see section 13 moderate expression: see section 17 weak expression: see section 18

upper jaw molar

strong

regional

strong expression: see section 08 09 20 21 moderate expression: see section 07 weak expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9299
Entity Detected:	Spock1, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 ( MGI:105371)
Sequence:	sense strand is shown >T9299 AACGAGCCGGGCATCAGCCTTCCCAGCCACCGCGGTACAGGAACCCCAGCAACTCGGGCGCCGGGACTCG GGCGGGCTCCCAAGATGCCAGCGATCGCGGTGCTCGCGGCGGCCGCCGCGGCGTGGTGCTTCCTCCAAGT GGACAGCCGGCACCTGGACGCGCTGGCCGGTGGCGCGGCCCTCAACAACGCCAATTTCCTAGACAATGAC CAGTGGCTGAGCACTGTCTCCCAGTATGACCGTGACAAGTACTGGAACCGCTTCCGAGATGAAGTTGAGG ATGACTATTTCAGAAACTGGAATCCCAACAAGCCCTTCGACCAAGCCCTGGACCCATCCAAGGACCCCTG CCTGAAGGTGAAATGCAGCCCGCACAAAGTATGTGTGACCCAGGACTACCAGACGGCTCTGTGTGTCAGC CGCAAGCACCTGTTGCCCAGGCAGAAGAAGGGCAATGTGGCTCACAAACACTGGCTTGGACCTTCCAATC TGGTTAAGTGCAAGCCTTGCCCCGTGGCGCAGTCAGCGATGGTCTGCGGCTCTGACGGCCACACGTACAC GTCCAAGTGCAAGTTGGAATTCCACGCTTGTTCTACAGGCAAAAGCCTCAACTCCCTCTGTGATGGGCCC TGTCCGTGTCTGCCTGAGCCTGAGCCACTGAAGCCCAAAGCAGAGAAGAGTGCCTGCACGGACAAGGAGC TGCGGAACCTCGCCTCCCGGCTGAAGGACTGGTTCGGGGCTCTTCATGAGGACGCCAATAGAGTCATCAA GCCTACCAGCTCTGATCCAGCCCAAGGCAGGTTTGACACCAGCATCTTACCCATTTGCAAGGACTCCTTG GGTTGGATGTTCAACAAGTTGGACATGAACTATGACCTGTTGCTGGACCACTCAGAGATCAATGCCATCT ACCTAGACAAATATGAGCCCTGCATCAAGCCTCTCTTCAACTCGTGCGACTCCTTCAAGGACGGCAAACT CTCCAACAATGAGTGGTGTTACTGCTTCCAGAAGCCAGCGGGTCTCCCTTGCCAGAATGAAATGAACAGA ATTCAGAAGCTAAGCAAGGGGAAAAGCCTACTGGGGGCCTTCATCCCTCGATGTAACGAGGAGGGCTACT ACAAAGCCACACAGTGCCACGGCAGCACGGGGCAGTGCTGGTGTGTGGATAAATATGGGAACGAGCTGGC CGGCTCCAGGAAACAGGGCACTGTAAGCTGCGAAGAGGAGCAGGAAACCTCCGGGGACTTCGGCAGTGGA GGCTCCGTGGTCCTGCTGGATGACCTAGAGGATGAGCGGGAGCTGGGACCAAAGGACAAAGAAGGGAAGC TGAGGGTGCGCACCCGGGCCGTGCGGGAAGATGATGAGGATGAAGATGACGACAAAGAAGATGAGGTCGG GTACATATGGTAGTGCCCACGAGGAAGAGGACACACTTTTGGCACAGATCTGCAAGTCGTTTCCTTTGCC TGCATTTGTATCTAAGACTCCGAGGCACCGGGGTCTCTTCTCCACTGTTGATCTCTGAACCGGGGCCTGA GGTTTGGAAGACCCTCTTCCCAGAGTGACTGAATTTGCATACGGTTGTGTGGGAGAAAGGATGTTATTTT TGTTCCTTGTTTTCGTTTTCATTGGATGAGAGGACAGGCTGGCTTAGTTAAAGCCGCCTCCTCTCCCTGT GAGGTTTTCGCAACTAGCATGTGATTTGTGTGGAATCCAACAGTGCGGGGAGCCCCACCCTCTCAAGCGT CAAAGACCCTCTCTGATTACCCACGTCGGTGGTTCCTACAGCATGGTTCCCAGCGTCTTATGATGTCTGC GTGCTTTTCTCGTGTCCTGTAGATGTTGTGGAAAACATACCAGGCTGTCCCTTTTCCCTGTCTCTACCAC CTCTGGTGTTGATTATTAAAAATTAATTTTCATGTCACTGTATCTGACTTCCTACAAACGACAGCCTTAA TTCAGTCCAAGTCCCTTTGGAGAATTCATTTTCTTTCTTAAAAAAAATAAGATCTCGACACCCCCCCCCC CCCCCTGCGCCCAAAGGTGACTGTGCTTGCATGACTTGCATACACACACCTTTCAGTCTAGTGTCTGGTT GTATACCAAAAACACCTTAGATAAGCAATGGCTTTTAGTCAGTTGCTCCAGACCGAGAACACAAACCGGG GTATGAAATTCCTCTTAGAGTCCGGTTTGCACTAGCTCTTCGTGTGTGTGTGTAGCCTGCATCCCGGGGA GGCTCTTCTATGCGGTCTCAGAGTGACTGGAAGCCTTTTTGATTTGGATGAGCCTCCATTTGCCTAAATT GTTTTTACCCACAGAAGCCATGCAATTCCCACGTGGGCAGCTTCACAAGCCATTTCTTCACTTCCGCTCA CAGCTCCCCTCAGGATACAGTGTACCCACCTGCGGCAGCTTCCTTCCCTATCCACCCAAGGGCTTTTTCC TACCGTGTTCCCCACTGAGCCCCTGCTCCCCAAGAAGACAGCATCATTATAAAGCTTAGAAGAAAATTTA GGGTTCTTCAGTGTCTCCTTTGACCCCCTTTCTATCCACAAATCATGACCCAGGGTATAGAGACTTTAAA GGTTTCTCGTGTTCCCCCATTATTTACTAGGGACCCCCAATGGAAGCCCAGAAAGAGATTCCTAGTAGCA CAAGAGGCTAGTCTCCTCATCAGCAGCCATCTCCCCGACACATGCTGACCTTAGTTGACACACGGCTGAG GCCATTTTACTGTTTGTCCTGAGAGCTCGTTATTGGCATCTGGTTTCACTAGTGATATCATCTCAATACT TTTCAGATTTCTTTCACAAGTACCAAAGAGCAATCTTCAGTGCGGGCCCCGTTCAGCTCAGGGCCAATCC CCCAGCTCTGTCTTCTTGCTTCCTGCCTAGCTCCTTGCCCTAACCAGCCACAGCCTGCCAGACACACTCT CTTCATGGTCCTTGCCTTCCCCCATGGTGAGTTTTAGCCAGTCCACAAGACAGATCCATCAACTCAGGCT ACAAAGGTATTTGGTTAAACCGAGATCAGGGAGGCAGAGCCCTAAACTGGCTTTCTGTATTTAGGAAGCC ACCTACAATTCGAATTCCCCTTCTTGAATAGGACAGGCAAAGTGACATCAGCCCTCTTGGGAAATGAATA TACCCGGTGCCCTTTACCCCTGGCCTGGGATACACACGCAGAGCCAGGGTGCTGAGATTCTGTGTGCTTC CTCCTTATGGAGATGGGAACCTGCTTTGGAATCTCAGTGCCATCCCCCCCGCCCCCACCCCCTATTTTGT TTGTTTTTTTCTTCCCATTCTCAAAATGGATCATAATTTAGAAAACAGGTGCTTAATCACAGCTAAATTG TAATAGAATACATTAAAAAGACATATTAGAAATCTCAGTTTAGGCCATCCATATAAGCCATTGTTGTTAT TTCCTATGAGCTTAGGGGGAAAAAAAACATGCACAAGAGAACAAATTAACAATCGGCAAATTGATTTCTC TCTGAGAAACTTCTAGGAACCCAATGAGCCACTTACTTTGTCTAATTATCTTATGACAAGTGTCACATTA AGATGACACTTAGAAGTCCTCAACATTATCTTAATGGTGAAGAAGAGTACTCAGGAGGCAATTCCCAGGA AGGTAATGAGATGTCATTTCCAGAGACATTCCAAGAAGATATTTTGATTCATTAAAATATTAAATCAAAA GCCCTCCTACGTGTGGAGCCCACATCAACAAGACAGGCCAGTGCCGCTTCTCATGGCCCCACTTTGGGTT TTAAAGATGGCTATTTATAATGGTACTTTAAAGTTAGACTTTTGTTCTGTTTCCTGAGAAAGAGTGCTTC CACTGGTCTTCTGGAAGGACCCTTTCAGTCTAATCATCAGGCGAGCATATGAGACTTTTTAAGCATATGC GGCTGTAATGATAAACCACTTGCTTAGGCAGGCACTGAGCTGAGTCCCCACTTAGCTTTAGAAAACCTTC AGTTGATTCTGTTACTCTAATTCTACAAACTGTTACTTACTATCATTCCTGTGACATCCAGTTTAAACCC TAGAATTCTCTTCCACCAGGGTTCATTGTCTGCCATATGACTATGTTTCTACAGATATTCTCAGCATGAG GATGGCTTCACATGGCTTTCTCTCAGTACTCCCCTTTATGCCCACGACCCTGTGTATACAGGAAATGATG ATTTGTCTGTCCAGGTCTACCCTCTGCTTGTCCCAGAACGCCATTGACATTGACTTTGGTTTGGTTTTTA TCTTGACCACACTGTACAGTAACATCCAAGAGCCCTTCCTACAGTGGGTGTTTTTTTTTTTTTTTTTTGG TCTTTTTACACCTTTTCTCGGTCAGTGATGATGTTTGTCCCTGTTCAACATGTAGCCCTGTAACAGAAGC CCATCAAATTAATTCAGAGTGTCAGTCTGTTGTCCCTATTGTAGGTGAAATAGTGACGTAGAAAACCTAG TATTCTGAATGCTTTTCCATGTAGACTTTTTCTGGAATGTGAACACGACTCTTCAGTTAATAGCAAGCGC TTCCCTGTAGCCCTGAGTAGGCGCAGTTCTGTCTGTCTCAATAAATTTTACTTTGTCCGCAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6842221 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6842221 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12393 same embryo
	EMAGE:12392 same embryo
	EurExpress:euxassay_008541 same experiment
	MGI:4828421 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source