EMAGE mouse gene expression spatial database

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EMAGE:12392

Gria1 glutamate receptor, ionotropic, AMPA1 (alpha 1) ( MGI:95808)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008537_01	euxassay_008537_02	euxassay_008537_03	euxassay_008537_04

euxassay_008537_05	euxassay_008537_06	euxassay_008537_07	euxassay_008537_08	euxassay_008537_09

euxassay_008537_10	euxassay_008537_11	euxassay_008537_12	euxassay_008537_13	euxassay_008537_14

euxassay_008537_15	euxassay_008537_16	euxassay_008537_17	euxassay_008537_18	euxassay_008537_19

euxassay_008537_20	euxassay_008537_21	euxassay_008537_22	euxassay_008537_23	euxassay_008537_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12392_wholemount_strong.wlz

12392_wholemount_moderate.wlz

12392_wholemount_weak.wlz

12392_wholemount_possible.wlz

12392_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12392_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex mantle layer

strong

regional

strong expression: see section 10 11 12 13 16 17 18 19

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 16 17 18 19

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord mantle layer

strong

homogeneous

strong expression: see section 10 11 12 13 14 15 16 17 18

saccule

moderate

regional

moderate expression: see section 05 06 07 08 19 20 21 weak expression: see section 22

neural retina

moderate

regional

moderate expression: see section 01 03 weak expression: see section 02 04 24

palatal shelf

moderate

regional

moderate expression: see section 07 08 09 10 19 20 21 weak expression: see section 11 12 13 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9298
Entity Detected:	Gria1, glutamate receptor, ionotropic, AMPA1 (alpha 1) ( MGI:95808)
Sequence:	sense strand is shown >T9298 GAAGAATCAAAGGGAGGGGAGGGAAGACCAAATCTATGGTTGGACCAGGGCTTCTTTTTCGCCAATGTAA AAAGGAATATGCCGTACATCTTTGCCTTTTTCTGCACCGGTTTTCTAGGTGCGGTTGTGGGTGCCAATTT CCCCAACAATATCCAGATAGGGGGATTATTTCCAAACCAACAATCACAGGAACATGCGGCTTTTAGGTTT GCTTTGTCACAACTCACGGAGCCCCCCAAGCTGCTTCCCCAGATCGATATTGTGAACATCAGCGACAGCT TTGAGATGACTTACCGATTCTGTTCCCAGTTCTCCAAAGGAGTGTACGCCATCTTTGGATTTTATGAACG AAGGACTGTCAACATGCTGACCTCCTTCTGTGGGGCCCTCCATGTGTGCTTCATCACTCCAAGTTTTCCC GTTGACACATCCAATCAGTTTGTCCTTCAGCTGCGCCCGGAACTACAGGAAGCTCTCATTAGCATTATCG ACCATTACAAGTGGCAGACTTTTGTCTACATTTATGATGCTGACCGGGGCCTGTCAGTCCTGCAGAGAGT CTTGGATACAGCCGCCGAGAAGAACTGGCAGGTGACGGCTGTCAACATTCTAACAACCACGGAGGAAGGA TACCGGATGCTCTTTCAGGACCTGGAGAAGAAAAAGGAGAGGCTGGTGGTGGTGGACTGTGAATCAGAAC GCCTCAACGCCATCCTGGGCCAGATTGTGAAGCTAGAAAAGAACGGCATCGGGTACCACTACATCCTCGC CAACCTGGGCTTCATGGACATTGACTTAAATAAGTTCAAGGAGAGTGGAGCCAATGTGACAGGTTTCCAA CTGGTGAACTACACAGACACGATCCCAGCCAGAATCATGCAGCAGTGGAGGACAAGTGACGCTCGGGACC ACACCAGGGTGGACTGGAAGAGGCCAAAGTACACTTCTGCTCTTACCTATGATGGTGTGAAGGTGATGGC GGAGGCCTTCCAGAGCCTGCGGAGGCAGAGGATTGACATATCCCGGCGAGGGAATGCTGGGGACTGTCTG GCTAACCCAGCTGTGCCCTGGGGCCAAGGGATCGACATCCAGAGAGCCCTGCAGCAGGTGCGCTTTGAAG GTTTGACAGGAAATGTGCAGTTTAACGAGAAAGGGCGCCGGACCAACTACACCCTCCATGTGATCGAAAT GAAGCATGATGGAATCCGCAAGATTGGTTACTGGAATGAAGATGATAAATTTGTCCCCGCAGCCACGGAC GCTCAGGCTGGAGGGGACAACTCAAGCGTCCAGAATAGAACCTACATCGTCACGACTATCCTCGAAGATC CTTACGTGATGCTTAAAAAGAATGCCAACCAATTTGAAGGCAATGACCGCTATGAGGGCTACTGCGTGGA ACTGGCTGCGGAGATCGCCAAGCACGTGGGCTATTCCTACCGACTTGAGATTGTCAGCGACGGCAAATAC GGAGCCCGGGATCCTGACACAAAGGCCTGGAATGGCATGGTGGGAGAGCTAGTCTATGGAAGAGCAGATG TGGCGGTGGCCCCCTTGACCATAACCTTGGTCCGGGAGGAAGTCATCGACTTCTCCAAGCCATTCATGAG TTTGGGAATCTCCATTATGATTAAGAAGCCACAGAAGTCCAAGCCAGGTGTCTTCTCCTTTCTTGACCCT TTGGCCTACGAGATCTGGATGTGTATAGTGTTTGCCTACATTGGAGTGAGCGTCGTCCTCTTCCTGGTCA GCCGTTTCAGTCCTTATGAATGGCACAGTGAAGAGTTTGAAGAAGGACGAGATCAGACAACCAGTGACCA GTCAAATGAGTTTGGCATATTCAACAGCCTGTGGTTCTCGCTGGGGGCCTTCATGCAGCAAGGATGTGAC ATTTCCCCCAGGTCCCTGTCTGGACGCATCGTCGGCGGTGTCTGGTGGTTCTTCACTTTGATTATCATCT CCTCATACACAGCCAACCTGGCTGCCTTCCTGACTGTGGAAAGGATGGTGTCTCCCATCGAGAGTGCAGA GGACCTGGCAAAGCAGACGGAAATTGCTTATGGGACATTGGAAGCAGGATCCACTAAGGAGTTCTTCAGG AGGTCTAAAATCGCTGTGTTTGAGAAGATGTGGACATACATGAAGTCTGCAGAACCGTCTGTGTTTGTTC GGACCACAGAGGAGGGCATGATCAGAGTGAGAAAGTCTAAAGGCAAATATGCCTACCTCCTGGAGTCCAC CATGAATGAGTACATTGAGCAACGCAAGCCCTGTGACACCATGAAAGTGGGAGGTAACTTGGATTCCAAA GGCTATGGCATTGCAACACCCAAGGGGTCCGCCCTGAGAAATCCAGTAAACCTGGCAGTGTTAAAACTGA ACGAGCAGGGGCTTTTGGACAAATTGAAAAACAAATGGTGGTACGACAAGGGCGAGTGCGGCAGCGGGGG AGGTGACTCCAAGGACAAGACCAGTGCTCTGAGCCTGAGCAATGTGGCAGGCGTGTTCTACATCCTGATT GGAGGGCTGGGATTGGCCATGCTGGTTGCCTTAATCGAGTTCTGCTACAAATCCCGTAGCGAGTCGAAGC GGATGAAGGGTTTCTGTTTGATTCCACAGCAATCCATCAATGAAGCCATACGGACATCGACCCTCCCCAG GAACAGCGGGGCAGGAGCCAGCGGAGGAAGTGGCAGTGGAGAGAATGGCAGAGTGGTCAGCCAGGACTTC CCCAAGTCCATGCAATCCATTCCCTGCATGAGCCACAGTTCAGGGATGCCCTTGGGAGCCACAGGATTGT AACTGGAGCAGACAGGAAACCCTTGGGGAGCAGGCTCAGGCTTCCCCAGCCCCATCCCAAGCCCTTCAGT GCCAAAAACAAAGAAATGAAACACCGCCTCCAACCACCACAACCATATGGAGGGCAATTCAGCCAATGTC CCTGAAGAATTAAAAAAAAAAAAAAAATTCCACTAGACCACCATCCCTTTTGTTTTGGGATTTTTTTTCA ACCTTTTCCATTTGCTGAGTGAAGATGGTCACTAACTCTATGCTGCAATAACGGGAGAGATCCTGTCCAA GGAAGTCTAACGTCTATAAAAATGGAGTCACTGGGATATCAATGAAGAAATCAAACTGTTTAATTTTAAT TCAGTTGTTAATGTGTCTTAGTGTGTGCATTATTTTCTTACTATTAACCCATGGTTTGCAGGCTCTGTTC AACCCTTTTCCTTCTTCCTATCCCCAGCCCCCACCCCCACCCCACCCCTTCCCATCACTTTTCAGTTTCA GATTGAAGACTCACGATTGGTTCCACCTTACAAGCAAGGAAAGGAATATTGACCTTCAAACTCATTCAGT GTTGGAGCTCTCTATGAGGCCTTCTATCCTTTGGCCCATGCCTTGGATGGAGACAGACACTACTGGAGAA GGGGGCTGCCTTCCCCAGAAAGAATACTACCTAAATGCTGGTTCATCAGAGAGTAACTGTGAAGAGCATC TCAGATAAGGGTTCTTAAAAGGGGGGATGGGCAGAGTACAGTAGAGGAGGACTTGTTAGGTTCATTGTTC CCCGGAAGAAGCTTCCTGCAATGCCCAGGAGCATCCCACAGGCAATAGAGTACCAATATGGTAGACCTCT TTCACAGTCAATCTCAGCCCTGGAAATTATGTTCTACTATGCTCAGGCCCAAAGGCAATGTGGGGGCCAA AGGGCAACTGGCCTTCTGACCCACTAGAGCCTTGCAAAGAGTTGGCAGTCAGGGGGGTGTCATACATGAT TCTTGCCTTTTCCTTTGCTTGATGTCTATACTGGATCCCCATGGTGGAGTAGGACTCTCTGTGGAGCCTC TGGCATAAGAGGAAATGTTGCCCGAGCTAAGAGCAAAAGGAAGGTGATAGGGAATTATGGAAGTCCATCC AAGCCCCTGACACAGAGCCTTCTGTGCTGTTTCCTGTCCTAGGTTTGAGAGACCAAATATGAACTTGTGA GTTGTGTCAATGCAGAGAACCGGGGTTTTGTGTTAATAGAGTATTGTTTTAGCATGTGATCAGATCTTAA GAAAGAAAGAAAGAAAGACAGCAAAGCCTGAATTCTTTTTGAGAGATGGAGATGAGGTACATCAGGGGAC CTTAAGATGCCTGAGCCCATCTTTTAACTCTAACACAGGGATACTAACCTTTTTCCCTGGCTAAGACAGA CTGAGGGCATTGCATTACAATGGCCAGAAACTACACCCATGAGTGCAACGACCAAGGTGAAGGACAGCCA GACAGGAAAGGTGACAAAAGCCACCCTGAGTGATACTAGGCTGCCTGAACCTTTGGGTGGGAGTGGAAGT CCCTAGCACACAGCTTTGTGGAGACCTCCACTCTCACTCTTGGTGCAACTGACTTCCACTTCTTATGCAG CCACTCCCAGGTGCATGCTTCAGTGAGTGCCACATGCCACTAGCTTTTACCAATAACCATATTCTGGCTT ATTCTCCTACCCTCTGCTTCCATTCAATCTTCCCCACTAGGGGTTATCATTGGCAATGGACGAGCTGAAG ATTTTGGAGCCTATCCATGGTTCAGGGCAGTAGAACTGGCTTCCTCTATGGATGCTTCCAGCGGACAACC ACCATCTCTGTGTCTCTCTCATTCTCTCTTCACCTGCCATAGCAAAGAAACATCCCAGAATCGACCCTTC TCCCATTGATCCATGTCTCCCGTGTCACCATGGGCATGGCATGTGAAGCAATGTCCTGCAGATGGATTAC CACCTTTTATTTCTGCCAGCTAACATCCTCCCAGCCTCCTGCCTACTACTGTAGACTCCGGTGTGACCCA CCACTGGGGAGTTCACACCTAGAAAGAGATGGAACTAAACTCTACTATCAGCTGCTTCAAAAACCTTTTG TCTACGGCCTGGGAAGATGTTCTCCAGCCCCTGAGCCTATTGGTTGGATAGAGAGAGACTGTGGTGGAAA TGAGGGTCTTGCTGGGAGGGGTCCATGACTCCGGGGAAGACGCTTCTCAACGAGGGATGTGTGGAAAGAA AACAGAAGTGAGAAAGAAGTTACCTTGTATTATGTATTTTTACTACACTTTTCTTAAAAATGGAGCAGTG GGAAAACTCTGAAAGAGATTGACATTTTTCTCAACAGGAATCCATACTTAACAGTTCTGGCTTTCATTAA ATTTTGCTCTTTGGTACCTGGGCCTTTTATTTAACATATATATTTATTTTAACTCTCTTGGAAGATGTGT GAGGGAAAAAAATAACTCTCATTTGATCAGACACTAAATATTTGGGGTAGGGATTTCTGTCTTATTTTCA AATAGCCAGGGTTTTTCTTCATTTTTTTGGTATTTGCATAAAGTGAACAATATCACAAGAATTAAATCAC TGTGGACCCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6842391 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6842391 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12393 same embryo
	EMAGE:12391 same embryo
	EurExpress:euxassay_008537 same experiment
	MGI:4825226 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source