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EMAGE:124

Alx3 aristaless-like homeobox 3 ( MGI:1277097)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:124
Figure 2D of ten Berge et al., 2001 [PMID:11532916] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:124EMAGE:124Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
124_voxel_strong_3D_1.wlz
124_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:124_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component mesenchyme
detected detected
regionalExpression is medial.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1277367
Entity Detected:Alx3, aristaless-like homeobox 3 ( MGI:1277097)
Sequence:sense strand is shown

>MGI:1277367
CCATGGAAATGTGGCTGGCTTCATGGGAGTGCCAGCCTCCCCTGCAGCCCACCCTGGCATCTATTCCATC
CATGGCTTCCCTCCTGCCCTGGGAGGGCACAGCTTTGAGCCTTCTCCGGATGGTGACTACAAGTCCCCAA
GCCTCGTTTCACTCAGGATGAAGCCCAAAGAGCCCCCTGGCCTGCTGAACTGGACCACGTGATGGGTTAC
ATGGGCACAGACTGAGCTGTCCACTTCTCTTCCTGCTCTGACCTGCTCCTAGCCCATCTCTACCTGGACA
CCAAACAGCAAACCCTTTTACCTCCATAACCTGGGTGGTTCTGGAAGCTTGGAAGAGGAGCTGGGACCCT
CAGCCTCCTGCAGGGAACAGGCTACAGGGTCTTATTTCTTAAAATAGGTGGGGCTGCCTAGGAGACAGGG
ACTTCAAAGCAGGTAGGACTCCTCCTTATAAAATGGCTCCCCTCATTTAAATTGGGCTGGAGAAAAGGTG
ACAGATGCCCCCTGTTTTCCAAAGACTGTTTCTAAGTCCAGGGAGCCCAGGAAGATGGGAGGAGGGTGCA
GACTAAACAGGGAGGCGCTCTCTGTGGTTCTTAGGGCTGGCCTCACTTTTGCCTTCCTACCACCAGGGGG
CGGTACGCTCAGCTCCTGGGACTGACAAGATCCCAGACGGTGAAGGTCAGCTCAATAATAGGTGGGGGGG
TCTCCTCTGTTCCAAGCATCCCTCACTTCTGCCCTGTGCTAGTCTACAGAGATACTATAAAGTCATTTTC
TCCAAGTAGGGAGAAGAAATTCCACCAAACATTAAATATTTGGATTTGCTTTCATGGGATCAGGGATATG
CCATGAAAACCATATTGGACATCGATCAAAATAAAAAAAAAATTTTTTTTCC
nt 979 - nt 1870 of U96109.1
Notes:The probe used in this study by ten Berge et al., 2001 [PMID:11532916] was originally described by ten Berge et al., 1998 [PMID:9676189] i.e. "A plasmid consisting of a 1.856-kb Alx3 cDNA cloned in pEXLox (Novagen) was cut with NcoI and transcribed with Sp6 polymerase. This results in an antisense RNA complementary to the 3'-untranslated region of the mRNA and the part encoding the C-terminal 66 amino acids." Editors note: Alx3 sequence U96109.1 was submitted to GenBank by ten Berge. Restriction cutting of U96109.1 with NcoI identifies 3 sites. A cut at the most 3' site (position 1048) would yield a fragment containing the 3'UTR and the most C-terminal 44 amino acids, whereas a cut at the second most 3' site (position 979) would yield a fragment containing the 3'UTR and the most C-terminal 66 amino acids as ten Berge states in their description. As such the sequence from 979 - the 3' end of the clone has been included here as the probe sequence..
Chemistry:RNA
Strand:antisense
Label:unspecified radioactivity
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Embedding:wax
Staining procedure:autoradiography
General Information
Authors:ten Berge et al., 2001 [PMID:11532916] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11532916] ten Berge D, Brouwer A, Korving J, Reijnen MJ, van Raaij EJ, Verbeek F, Gaffield W, Meijlink F 2001 Prx1 and Prx2 are upstream regulators of sonic hedgehog and control cell proliferation during mandibular arch morphogenesis. Development (128):2929-38
 [ doi:10.1006/dbio.1998.8921] [ PMID:9676189] ten Berge D, Brouwer A, el Bahi S, Guénet JL, Robert B, Meijlink F 1998 Mouse Alx3: an aristaless-like homeobox gene expressed during embryogenesis in ectomesenchyme and lateral plate mesoderm. Dev Biol (199):11-25
Links:MGI:2149998 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI