Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:1240

Nefm neurofilament, medium polypeptide ( MGI:97314)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:1240
Fig 2g Chi et al, 2003 [PMID:12736208] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: III, oculomotor nerve; IV, trochlear nerve; V, trigeminal nerve and ganglia; VII, facial nerve and ganglia; VIII, acoustic nerve and ganglia.
Expression Pattern Description
Spatial Annotation:
EMAGE:1240Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1240_wholemount_strong_3D_1.wlz
1240_wholemount_moderate_3D_1.wlz
1240_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1240_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
acoustic viii ganglion
detected detected
facial vii ganglion
detected detected
trigeminal v ganglion
detected detected
central nervous system
detected detected
regionalExpression in oculomotor nerve III, trochlear nerve IV, trigeminal nerve V, facial nerve VII and acoustic nerve VIII
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1330766
Entity Detected:Nefm, neurofilament, medium polypeptide ( MGI:97314)
Notes:The anti-NEF3 antibody used in this study by Chi et al, 2003 [PMID:12736208] is described as "2H3 antibody (Dodd et al., 1988 [PMID:3272160] ) supernatant (Developmental Studies Hybridoma Bank, U. Iowa)". Details below are taken from the Developmental Studies Hybridoma Bank online catalogue. This antibody detects a 165 kDa form of NEF3.
Antibody Type:monoclonal
Raised In:mouse
Supplier:Developmental Studies Hybridoma Bank
Catalogue Number:2H3
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Secondary Antibody:HRP-linked sheep anti-mouse
Labelled with:horse radish peroxidase
General Information
Authors:Chi et al, 2003 [PMID:12736208] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00487] [ PMID:12736208] Chi CL, Martinez S, Wurst W, Martin GR 2003 The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development (130):2633-44
 [ doi:10.1016/0896-6273(88)90194-8] [ PMID:3272160] Dodd J, Morton SB, Karagogeos D, Yamamoto M, Jessell TM 1988 Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons. Neuron (1):105-16
Links:MGI:2677311 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI