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EMAGE:1241

Nefm neurofilament, medium polypeptide ( MGI:97314)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:1241
Fig 3A Conlon et al, 1995 [PMID:7789282] . Copyright: This image is from Development and is displayed with the permission fo the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: short arrow, midbrain; long arrows, cranial ganglia.
Expression Pattern Description
Spatial Annotation:
EMAGE:1241Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1241_wholemount_strong_3D_1.wlz
1241_wholemount_moderate_3D_1.wlz
1241_wholemount_weak_3D_1.wlz
1241_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1241_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
facio-acoustic vii-viii preganglion complex
detected detected
Neurofilament staining was observed in the 5th and 7th cranial ganglia
trigeminal v preganglion
detected detected
Neurofilament staining was observed in the 5th and 7th cranial ganglia
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1330766
Entity Detected:Nefm, neurofilament, medium polypeptide ( MGI:97314)
Notes:The anti-NEF3 antibody used in this study by Conlon et al, 1995 [PMID:7789282] is described as the "monoclonal antibody 2H3 against the 160,000 Mr neurofilament protein (Developmental Studies Hybridoma Bank, Iowa)", "at 1:4 dilution".
Antibody Type:monoclonal
Raised In:mouse
Supplier:Developmental Studies Hybridoma Bank
Catalogue Number:2H3
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde in PBS for 2 hr
Secondary Antibody:peroxidase-linked goat anti-mouse IgG
Labelled with:horse radish peroxidase
General Information
Authors:Conlon et al, 1995 [PMID:7789282] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7789282] Conlon RA, Reaume AG, Rossant J 1995 Notch1 is required for the coordinate segmentation of somites. Development (121):1533-45
Links:MGI:1276151 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI