EMAGE mouse gene expression spatial database

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EMAGE:12419

Ltbr lymphotoxin B receptor ( MGI:104875)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011223. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011223_01	euxassay_011223_02	euxassay_011223_03	euxassay_011223_04

euxassay_011223_05	euxassay_011223_06	euxassay_011223_07	euxassay_011223_08	euxassay_011223_09

euxassay_011223_10	euxassay_011223_11	euxassay_011223_12	euxassay_011223_13	euxassay_011223_14

euxassay_011223_15	euxassay_011223_16	euxassay_011223_17	euxassay_011223_18	euxassay_011223_19

euxassay_011223_20	euxassay_011223_21	euxassay_011223_22	euxassay_011223_23	euxassay_011223_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12419_wholemount_strong.wlz

12419_wholemount_moderate.wlz

12419_wholemount_weak.wlz

12419_wholemount_possible.wlz

12419_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12419_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 20 21 22 23 24

humerus

moderate

regional

moderate expression: see section 02 03 04 05 06 07 22 23 24

mandible

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23 24 moderate expression: see section 14

maxilla

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 17 18 19 20 21 22 23 24

bladder

weak

regional

weak expression: see section 13 14 15 16 17

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 24 moderate expression: see section 23 weak expression: see section 07

clavicle

strong

regional

strong expression: see section 07 08 09 10 19 20 21 moderate expression: see section 11 18

tail mesenchyme

strong

regional

strong expression: see section 13 17 18 moderate expression: see section 14 weak expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36603
Entity Detected:	Ltbr, lymphotoxin B receptor ( MGI:104875)
Sequence:	sense strand is shown >T36603 CCTCCTACTCGGATACCATCTGTAAAAATCCCCCAGAGCCAGGAGCAATGCTACTGCTGGCCATCCTGCT GTCGCTGGTCCTCTTTCTGCTCTTCACCACTGTCCTGGCCTGTGCCTGGATGAGGCACCCGTCTCTCTGC AGAAAGCTGGGTACTCTGCTCAAGCGGCACCCAGAGGGAGAAGAATCTCCCCCTTGCCCCGCTCCAAGAG CCGACCCACATTTCCCTGACCTGGCAGAGCCACTCCTACCCATGTCTGGAGACTTGTCCCCATCCCCTGC TGGACCCCCAACGGCCCCCTCCTTGGAGGAAGTGGTGCTACAACAGCAGAGTCCCCTGGTCCAGGCCAGA GAGCTGGAGGCTGAACCTGGGGAACATGGCCAGGTGGCCCACGGTGCGAATGGCATTCATGTGACCGGGG GCTCTGTGACTGTCACCGGCAACATCTACATATACAATGGGCCAGTGCTGGGGGGAACACGGGGCCCTGG AGACCCTCCAGCTCCCCCTGAGCCTCCATACCCCACTCCTGAAGAGGGAGCTCCTGGCCCTTCTGAGCTG TCTACACCCTACCAGGAGGATGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90191. Forward Primer - name:090191_F_cDNA_Ltbr, sequence:CCTCCTACTCGGATACCATCTG; Reverse Primer - name:090191_N_SP6_cDNA_Ltbr, sequence:CCATCCTCCTGGTAGGGTGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12418 same embryo
	EMAGE:12417 same embryo
	EMAGE:12416 same embryo
	EMAGE:12421 same embryo
	EMAGE:12420 same embryo
	EurExpress:euxassay_011223 same experiment
	MGI:4826020 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source