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EMAGE:1242

Nefm neurofilament, medium polypeptide ( MGI:97314)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:1242 EMAGE:1242 EMAGE:1242
Fig 4A Dunwoodie et al, 2002 [PMID:11923214] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 4B Dunwoodie et al, 2002 [PMID:11923214] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 4F Dunwoodie et al, 2002 [PMID:11923214] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: (A) Lines mark region between forelimbs and hindlimbs. drg, dorsal root ganglia; sn, spinal nerve; scg, sensory chain ganglia. (B) Lines dorsal and ventral to somites mark individual somitic segments. (F) Dorsal view of A with anterior towards the top.
Expression Pattern Description
Spatial Annotation:
EMAGE:1242Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1242_wholemount_strong_3D_1.wlz
1242_wholemount_moderate_3D_1.wlz
1242_wholemount_weak_3D_1.wlz
1242_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1242_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
spinal ganglion
detected detected
Authors report a regular periodicity with which spinal nerves and ganglia form (see Fig 4A)
spinal nerve
detected detected
Authors report a regular periodicity with which spinal nerves and ganglia form (see Fig 4A)
dorsal root ganglion
detected detected
Authors report a regular periodicity with which spinal nerves and ganglia form (see Fig 4A)
segmental spinal nerve
detected detected
Ventral spinal axons pass through anterior of somite segments (see Fig 4B)
tail neural tube
detected detected
see Fig 4F
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1330766
Entity Detected:Nefm, neurofilament, medium polypeptide ( MGI:97314)
Notes:The anti-NEF3 antibody used in this study by Dunwoodie et al, 2002 [PMID:11923214] is described as "anti-neurofilament monoclonal antibody 2H3 (Developmental Studies Hybridoma Bank)".
Antibody Type:monoclonal
Raised In:mouse
Supplier:Developmental Studies Hybridoma Bank
Catalogue Number:2H3
Specimen
Organism:mouse
Strain:129P2/Ola x C57BL/6
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Dll3tm1Rbe/Dll3+
Preparation:wholemount
Procedures
General Information
Authors:Dunwoodie et al, 2002 [PMID:11923214] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11923214] Dunwoodie SL, Clements M, Sparrow DB, Sa X, Conlon RA, Beddington RS 2002 Axial skeletal defects caused by mutation in the spondylocostal dysplasia/pudgy gene Dll3 are associated with disruption of the segmentation clock within the presomitic mesoderm. Development (129):1795-806
Links:MGI:2178443 allele description
 MGI:2178632 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI