EMAGE mouse gene expression spatial database

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EMAGE:12424

Pcdhga6 protocadherin gamma subfamily A, 6 ( MGI:1935218)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011226. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011226_01	euxassay_011226_02	euxassay_011226_03	euxassay_011226_04

euxassay_011226_05	euxassay_011226_06	euxassay_011226_07	euxassay_011226_08	euxassay_011226_09

euxassay_011226_10	euxassay_011226_11	euxassay_011226_12	euxassay_011226_13	euxassay_011226_14

euxassay_011226_15	euxassay_011226_16	euxassay_011226_17	euxassay_011226_18	euxassay_011226_19

euxassay_011226_20	euxassay_011226_21	euxassay_011226_22	euxassay_011226_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12424_wholemount_strong.wlz

12424_wholemount_moderate.wlz

12424_wholemount_weak.wlz

12424_wholemount_possible.wlz

12424_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12424_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9310
Entity Detected:	Pcdhga6, protocadherin gamma subfamily A, 6 ( MGI:1935218)
Sequence:	sense strand is shown >T9310 GTTTTTCCCACCAGCAATCGCTGGGAAGTCTTTAAAAAGCCAGTCATGGCGGCTCTGCAGAGGCGCCCGC CCTGCTGCGAGTTGCTCCTGCTTCTCAATCTCCTGTGGTGGTTGTGGGGCTCAGAAGCTGGGCAGCTCCG CTACTCTATTCCCGAGGAAGTGGAGAAAGGCTCCTTCGTGGGCAGCATCGCCAAAGACCTGGGGCTGCAG CCCCGCGAGCTGGCAGAGCGCGGGATTCGCATCATCTCCAGAGGTAGGTCGCAGCTTTTCTCCCTAAACC AGCGAAGCGGCAGCTTGGTCACCGCGGGCAGGATAGACCGGGAGGAGCTGTGCGCCCAGAGCGCGCCCTG TCTTGTGAGCTTTAACATCCTTATAGAAGATAAATTAAATCTTTATCCTGTGGAAGTAGAAATAGTGGAC ATTAATGACAATGCACCCAGATTCTTAAAGGAAGAAATGGAATTGAAGATTCTTGAAAATGCAGCTCTAT CCTCTCATTTTCTACTAATGGGTGTCTATGACCCCGATGTGGGAGTGAACTCCATTCAGGGCTTCAAGCT CAGCGGTAGTAGTCACTTCTCAGTACATGTGCAAAGCCAAGACCATGGGCCCAAATACCCGGAGCTGGTG TTGGAGCACAGCCTGGACAGGGAAGAGGAAGCCGTTCACCACTTGGTCCTTGTTGCCATGGATGGTGGTG ACCCTGTCCGAACAGGCATGGCGCGAATTCTTGTAACTGTCGTAGATGTGAACGACAATGCTCCAGTATT TACTCAGCCTATCTATCGTGTGAGTGTCCCTGAAAACCTGCCTGTGGGTACACGAGTGTTAACAGTTAAC GCCACCGACCAGGATGAAGGAATCCATGCAGAAATAACATATTCCTTTGTGAGGATCACAGAAGAAATCT CGCAGATTTTCTGCTTGAATCCTTTAACTGGGGAAATTTCCACTTCCGAAACTCTAGACTATGAGGATTC AAGATTTTATGAGCTGGATGTTGAAGCCCGAGATCAATCAGATCTTCAAGACCGAGCAAAAGTTTTAATA ACCATCTTGGACATGAACGACAATGCACCGGAAGTAGTGGTTACATCTGGTAGCAGAGCAATTGCTGAAA ACTCACCTTCAGGGACCGTAATTGCTCTTTTTCAAGTCTATGATAAAGACTCTGAACGGAATGGCCTGGT AATATGCTCCATATCAGAAAGTCTCCCATTTAAATTGGAAGAGTCATTAGACAATTATTTTCGGTTAGTG ACAAATACAGAACTGGATCGCGAACAGGTATCTTCGTATAACATCACTGTGACAGCCACTGACAGAGGAA CACCACCTCTGTCAACAAAAATATTCATTTCCTTAGATGTGGCAGACATTAATGACAACCCACCTGTTTT TTCCCGTTCATCCTACTCTGTCTATGTCTCTGAGAATAACCCTAAGGGCGTCTCCATCTTCTCTTTGAAT GCAGTGGACCCTGACAGTGAAGAAAATGCAGAAATTATTTACTCTCTGGCCAAAGAAACCCTTCAGGGAA CACCTCTATCCTCCTTCCTCTCCATCAACTCCATCACTGGTGTCCTGTATGCACTGTGTTCCTTTGACTA TGAGCAGTTTAGAGAACTGAATCTACTGGTGACAGCCAGTGACAGAGGGAAACCCCCACTTAGCAGCAAT GTGTCACTTAACCTGTTTGTGCTGGACCAGAATGACAATGTACCTGAGATTCTGTACCCTGTTCTCCCCA CGGATGGTTCTACTGGTGTGGAGCTGGCCCCCCGCTCTGCAGAACCTGGATATCTAGTGACCAAAGTGGT GGCAGTGGACAAAGACTCAGGTCAGAATGCCTGGCTGTCCTACCGCCTGATCAAGGCCAGTGAGCCAGGG CTCTTCTCAGTGGGACTACACACAGGTGAGATCCGCACAGCAAGGGTCCTGCTGGACAGAGATGCTCTCA AGCAGAGCCTGGTGGTGGCTGTGCAGGACCATGGCCAGCCGCCTCTCTCAGCTACTGTTACACTTACTGT GGCTGTGGCCAGCAGCATTCCTGACATCCTGGCTGACTTGGGCAGCCTTGACCTTCCACACAAGTCTGAA GATTCAAGCCTTACAATCTACCTGGTGGTGGCGGTGGCCACTGTTTCGTGCATCTTCTTTGCTTTTGTCA TGGGGCTGCTGGTGCTCAGGCTGTGGCACTGGCACAAGTCAAGATTGCTGAAGGCCACAAGGAAAGGTGT GTCAAATATGTCCACTTCACATTTCGTGGGTATTGATGGGGTGCAGGCTTTTCTACAGACCTATTCCCAT GAGGTCTCCCTCACAGCAGACTCCAGGAAGAGCCATCTGATCTTCCCCCAGCCCAACTATGCCCACACAC TCATCAGTCAGGAGGGCTGTGAGAAAAATGAGCCTTTATTGATACAGGAAGACTCGGCTTTTTGCAAAGA GGAAGACTCTCTTGATCAGCAAGCCCCGCCCAACACTGACTGGCGTTTCTCTCAAGCCCAGAGACCCGGC ACGAGCGGATCCCAAAATGGTGATGAAACTGGCACCTGGCCCAACAACCAGTTTGATACAGAGATGCTGC AAGCCATGATCTTGGCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCCTGGGAGGGGGCGCTGGCAC CATGGGTCTGAGCGCTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCCTGACTACCGCCAGAACGTG TACATCCCTGGCAGCAATGCCACGCTGACCAATGCCGCTGGCAAACGAGATGGCAAGGCTCCAGCAGGTG GTAATGGCAACAAGAAGAAATCGGGCAAGAAAGAGAAGAAGTAATATGGAGGCCAGGCCTTGAACCACAA GGCAGCCTCCCTCCCCAGCCAGTCCAGCTCGTCCTTACTTGTACCCAGGCCTCAGAATTTCAGGGCTCAC CCCAGGATACTGGTAGGAGCCAGGGCCATGCTCCCCGTTGGGAAACAGAAACACGTGCCCAAGCCAACAC CCCCTCTCTATACCCTAGGGAGTTGAATATGCAAAGAGAGTTCTGCTGGGACCCCCCTATTCAATCAGTG ATTGTACCCACATGGGTAGCAGGGTTTGTGTGTGTGGACATACACACACACACACACACACACACACACA AACCATGCCACACTCCCTTAGTTACAGCTGAACTCCTCCATCTTCCAACCCAGGCAGGCTTATCCATCTC CTGCCGCCCTCTCCCCACCCCACCCACTCCTTCAGTCCCTCTCTTTGAGCAAGGTAGCTGGGCTGTTGGG GTGACCGGTGAGCTAAGCCGGCTCCTAGTTCTGAACGGTAGGGAGAGTATTACAATCTTTTGGCCTCTCC TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTGGTACCAGTCTTCTCTTTTCCTTCCAGAGCCTGAGACCA ATGCTTAAGTTTTGGGGGAACAGGCACCCTCCCTCTGGTACTGATGATGCTTGCTGGATTTTGGGAAGGC ATTTTGCTACTAAGCCTCTTTTCAATGCCTGGGGACAACTAGTCTTTTGTTTTTCATTGTTTGATGTTCC CACTGCATGCTGTGACTTCCCTACACCTCAAAGAAGAGACTCCTTTGCATGTCTGGAGACAGTAGGGGGT GGTAAACTAAAGGGAAGTTGAGAGTGTATACTGCTGGGAGTGGGGCAGTGGACAAAACAGCCTGTTAATA GGGTCTTGCAAGGGGCTCTGTATGTACCCAGGGGACTGGCTCAATCCTCACATTCTAGCCATAGACAAAC ACCAGGCCAGACCCCTCCATTCTGGTTCAGCCTGGGCAGCTTGGGCTGAGCCACCAGGACCAATGGATTT AAGCTGACATTTCAGTCCAAGACGACGACTTCTAAGTGAGTTTAAGACCAGAGAGGAAAGAGGGGCCTCT GTGGGTGCTGGGTACTCCAGAGGTGCCCTTGGTGGGAGGACCAGTGGTCTTAGCAGGAAGGGGGGCCCAG CAAGGTCATTCTTGGACCCTGGGTCTAGTCCAGTAGCTAGAAAAAGGGACCAAGTGGCCATAAAGTCCCA GCCAATGATGGGGCTTTTCCAGTGGGCCCCTGTAGACCTCAAGCCCCTGGCCTCCACCTTACCAGGTGCC ATTTCTTCTCAGAAGGCCACTGCCCAGGCCCCCAGGCCGCCCCCTTGTGGCCAGAGAACGGTTAAAGCCC CCCAGTGCCTCCTTGTGCATAGAACTTCCTCTGCCCTAGCCCTTACAGTAGTGTAGAAGATCCCCCTCCC CCGCTGGTGTAGAATAGCCAATAGTATAGTGTGGTGTGCTTTTACGTGATGGCGAGTGGGCAGCGGGCGG TGGGCTGTACACAGCCGTCTGTCCTTTGAATCTCAATCTGCCTGCGCGGCCCGTGCTGTGTTTTGTGCTG TGTCCACACGCTGCGGCGACCCCTCCCTGTACTGACTTCTCTATAAAAAGCTTCTCTTCGCATAGTCACG TAGCTCCTGCCCATCCCCCTTCCGATATCTCTCGCAAGTTTTATACTCTAATATTTATATGGCTTTTTTC TTCGAAAATTAAAAATAAAAAAATGGTTTCTTCTGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6844625 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6844625 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12427 same embryo
	EMAGE:12423 same embryo
	EMAGE:12425 same embryo
	EMAGE:12426 same embryo
	EMAGE:12422 same embryo
	EurExpress:euxassay_011226 same experiment
	MGI:4827090 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source