EMAGE mouse gene expression spatial database

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EMAGE:12427

4833423E24Rik RIKEN cDNA 4833423E24 gene ( MGI:2687041)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011145. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011145_01	euxassay_011145_02	euxassay_011145_03	euxassay_011145_04

euxassay_011145_05	euxassay_011145_06	euxassay_011145_07	euxassay_011145_08	euxassay_011145_09

euxassay_011145_10	euxassay_011145_11	euxassay_011145_12	euxassay_011145_13	euxassay_011145_14

euxassay_011145_15	euxassay_011145_16	euxassay_011145_17	euxassay_011145_18	euxassay_011145_19

euxassay_011145_20	euxassay_011145_21	euxassay_011145_22	euxassay_011145_23	euxassay_011145_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12427_wholemount_strong.wlz

12427_wholemount_moderate.wlz

12427_wholemount_weak.wlz

12427_wholemount_possible.wlz

12427_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12427_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 09 10 18 19 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 16 17 18 19 weak expression: see section 13 15

lower jaw incisor

moderate

regional

moderate expression: see section 16 weak expression: see section 12

palatal shelf

weak

regional

weak expression: see section 15

palatal shelf epithelium

weak

regional

weak expression: see section 12

upper jaw incisor

moderate

regional

moderate expression: see section 16 weak expression: see section 12

upper jaw molar

moderate

regional

moderate expression: see section 07 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37340
Entity Detected:	4833423E24Rik, RIKEN cDNA 4833423E24 gene ( MGI:2687041)
Sequence:	sense strand is shown >T37340 TTTACAATGTGACTGACTGGGCTGGCAAGCATCCAGGTGGACGTCGGGTTCTCAACCACTATGCTGGGCA AGATGCAACGGATGCTTTCAGGGCCATGCACCTGGATCTGGGCATGGTGAAGCTGTATCTTAAGCCACTA CTCATTGGAGAACTTTCTCCAGAAGAACCAAGCCAAGAGAAAAACAAAAACGCACAGTTGGTGGAAGATT TCCGTGAATTGCGCAAGACGTTAGAGGCCATGAACATGTTCAGTGCCAACCTGCGGTTCTTCTTTCTGCA CCTTGCCCAGATCTTGATCTTGGAAATCTCAGCATGGCTAATACTACACCATTTTGGCAGCAGCTGGCTT GTTACAATACTTATTTCCTTTCTCCTTACAGTGTCTCAGGCTCAATGTTCATTTTTACAACATGACTTAG GACACCTTTCCATGTTTAAGAAGTCCAAGTGGAACCATCTGATGCACAAATTTGTAATGTGTCATCTCAA GGGCCTGTCAGCTGATTGGTGGAATTATCGACACTTCCAGCATCATGTAAAACCAAATATCTACCCCAAG GATCCAGACATTGATGTGGGCCCACTCTTTCTGGTTGGAGATACTCAGCCTATAAAGTATGGCAAGAAGA AAATAAAATACATTGACTATGAGAAGCAGCACCTGTACTTCTACATGGTTGCACTCCCCTTCCTCATGCC TGTCTACTTTAATTTGCAATCCATGCAAGTGATGTACCTTCGAAAATACTGGATGGACATTGCATGGGTC AGCAGCTTTTACATTCGTTATTTCATCACATTTGGCCCTTTCTATGGAATTTTCGGAACAGTGTTGCTCA TATATTTGGTCAAGTTTATTGAGAGCCCCTGGATTGCTTACGTTACCCAGATGAGTCACATACCAATGAA AATGAGCAGTGAAGAGAACCACGACTGGCTCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76672. Forward Primer - name:076672_F_cDNA_4833423E24Rik, sequence:TTTACAATGTGACTGACTGGGC; Reverse Primer - name:076672_N_SP6_cDNA_4833423E24Rik, sequence:CTGAGCCAGTCGTGGTTCTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12423 same embryo
	EMAGE:12425 same embryo
	EMAGE:12424 same embryo
	EMAGE:12426 same embryo
	EMAGE:12422 same embryo
	EurExpress:euxassay_011145 same experiment
	MGI:4822713 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source