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EMAGE:1243

Nefm neurofilament, medium polypeptide ( MGI:97314)
TS15 (9.25 dpc)
immunohistochemistry

Data Images
EMAGE:1243
Fig 4C Kawasaki et al, 2002 [PMID:11830568] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: Arrows indicate 2H3(Nfm)-positive fibres around the neural tube. da, dorsal aorta; fl, the forelimb; nt, neural tube. The authors specify that "The staining in blood cells and the internal surface of the digestive tracts is non-specific".
Expression Pattern Description
Spatial Annotation:
EMAGE:1243EMAGE:1243Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1243_voxel_strong_3D_1.wlz
1243_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1243_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
detected detected
regionalExpression is detected in nerve fibres near the neural tube
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1330766
Entity Detected:Nefm, neurofilament, medium polypeptide ( MGI:97314)
Notes:The anti-NEF3 antibody used in this study by Kawasaki et al, 2002 [PMID:11830568] is described as "rat anti-neurofilament monoclonal antibody 2H3 (Dodd et al., 1988 [PMID:3272160] ). This antibody is available from Developmental Studies Hybridoma Bank, U. Iowa and the details below are taken from their online catalogue. This antibody detects a 165 kDa form of NEF3.
Antibody Type:monoclonal
Raised In:mouse
Supplier:Developmental Studies Hybridoma Bank
Catalogue Number:2H3
Specimen
Organism:mouse
Age:9.25 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Secondary Antibody:anti-rat Ig (?)
Labelled with:Cy3
Visualisation method:fluorescence
General Information
Authors:Kawasaki et al, 2002 [PMID:11830568] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11830568] Kawasaki T, Bekku Y, Suto F, Kitsukawa T, Taniguchi M, Nagatsu I, Nagatsu T, Itoh K, Yagi T, Fujisawa H 2002 Requirement of neuropilin 1-mediated Sema3A signals in patterning of the sympathetic nervous system. Development (129):671-80
 [ doi:10.1016/0896-6273(88)90194-8] [ PMID:3272160] Dodd J, Morton SB, Karagogeos D, Yamamoto M, Jessell TM 1988 Spatial regulation of axonal glycoprotein expression on subsets of embryonic spinal neurons. Neuron (1):105-16
Links:MGI:2176238 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI