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EMAGE:12434

Klhl4 kelch-like 4 (Drosophila) ( MGI:2442829)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12434 EMAGE:12434 EMAGE:12434 EMAGE:12434 EMAGE:12434
"Pseudo-wholemount" of euxassay_011076. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011076_01 euxassay_011076_02 euxassay_011076_03 euxassay_011076_04
EMAGE:12434 EMAGE:12434 EMAGE:12434 EMAGE:12434 EMAGE:12434
euxassay_011076_05 euxassay_011076_06 euxassay_011076_07 euxassay_011076_08 euxassay_011076_09
EMAGE:12434 EMAGE:12434 EMAGE:12434 EMAGE:12434 EMAGE:12434
euxassay_011076_10 euxassay_011076_11 euxassay_011076_12 euxassay_011076_13 euxassay_011076_14
EMAGE:12434 EMAGE:12434 EMAGE:12434 EMAGE:12434 EMAGE:12434
euxassay_011076_15 euxassay_011076_16 euxassay_011076_17 euxassay_011076_18 euxassay_011076_19
EMAGE:12434 EMAGE:12434 EMAGE:12434 EMAGE:12434
euxassay_011076_20 euxassay_011076_21 euxassay_011076_22 euxassay_011076_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12434Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12434_wholemount_strong.wlz
12434_wholemount_moderate.wlz
12434_wholemount_weak.wlz
12434_wholemount_possible.wlz
12434_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12434_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart ventricle
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14 15 16 17
renal medullary interstitium
weak weak
regionalweak expression: see section 08 09 10 11 12 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36559
Entity Detected:Klhl4, kelch-like 4 (Drosophila) ( MGI:2442829)
Sequence:sense strand is shown

>T36559
ACGTGATGGAAGTTCCTGTCTTAAGTCCATGGAATTCTTTGACCCGCATACTAACAAGTGGAGTCTGTGT
GCCCCGATGTCCAAAAGACGTGGAGGTGTAGGAGTAGCAACACACAATGGATACTTGTATGTTGTAGGGG
GTCATGATGCCCCTGCTCCTAATCATTGCTCCAGGCTTTCTGACTGCGTGGAAAGATACGACCCAAAAGG
CGATTCATGGTCAACTGTGGCACCTTTGAGTGTTCCTCGAGATGCTGTTGCAGTGTGCCCCCTTGGAGAC
AAACTCTACGTGGTTGGCGGATATGATGGTCATACTTACTTGAATACCGTTGAGTCGTATGATGCTCAGA
AAGACGAATGGAAAGAGGAAGTCCCTGTGAACATTGGAAGAGCTGGAGCATGTGTTGTTGTTGTGAAGCT
GCAGTGAAAGTATCATTTGCTAATGCAATGAAATGGATTTCAAGAATGAAGAAAAACATACTCTTTTATG
AAGTTCATAAGCAAACTAAATGCTGTTGTGTCATTCTCACATATATTTGAAACCGTTAACATTCATAAGG
CTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67507. Forward Primer - name:067507_F_cDNA_Klhl4, sequence:ACGTGATGGAAGTTCCTGTCTT; Reverse Primer - name:067507_N_SP6_cDNA_Klhl4, sequence:CAGCCTTATGAATGTTAACGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12437 same embryo
 EMAGE:12436 same embryo
 EMAGE:12435 same embryo
 EMAGE:12433 same embryo
 EMAGE:12438 same embryo
 EurExpress:euxassay_011076 same experiment
 MGI:4825804 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS