EMAGE mouse gene expression spatial database

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EMAGE:12444

Loxl2 lysyl oxidase-like 2 ( MGI:2137913)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011221. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011221_01	euxassay_011221_02	euxassay_011221_03	euxassay_011221_04

euxassay_011221_05	euxassay_011221_06	euxassay_011221_07	euxassay_011221_08	euxassay_011221_09

euxassay_011221_10	euxassay_011221_11	euxassay_011221_12	euxassay_011221_13	euxassay_011221_14

euxassay_011221_15	euxassay_011221_16	euxassay_011221_17	euxassay_011221_18	euxassay_011221_19

euxassay_011221_20	euxassay_011221_21	euxassay_011221_22	euxassay_011221_23	euxassay_011221_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12444_wholemount_strong.wlz

12444_wholemount_moderate.wlz

12444_wholemount_weak.wlz

12444_wholemount_possible.wlz

12444_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12444_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9443
Entity Detected:	Loxl2, lysyl oxidase-like 2 ( MGI:2137913)
Sequence:	sense strand is shown >T9443 GCCGGGCTAAACAGGAGGCCGCCTTCAGGGCGAGCTGGGGTCGGGGGTGGGGTTTGCCGCTAGTTTCTCT ATAAAAGCCCCGGGAGAAAGCTTGCCCCCAGCTGTAGCGCGCGCCTCCCCGCATTTTCCCCGGCTTCTTG GCGGGAGGGCCCTCGGCGATCGGCGCGGAGAGGGGCCATCGCTGAGAGCCCAGTCCCAGCCTGGCCACCA CGGAGCCCAGCTGCCAATTCCTGCTGCCACCCACCACCCCCCTCTCAGCACAGGACAAGGATGGAGCTCC ATTTTGGCTCCTGCCTCTCCGGCTGTTTGGCTCTGCTTGTCTTGCTGCCTTCCCTGAGCCTAGCACAGTA CGAGGGCTGGCCCTACCAGCTCCAGTACCCTGAGTACTTCCAGCAGCCCGCTCCTGAGCACCATCAGCGG CAGGTGCCCTCCGATGTGGTCAAGATCCAGGTCCGCCTGGCGGGCCAGAAGAGGAAGCACAATGAGGGCC GCGTGGAGGTCTACTACGAAGGCCAGTGGGGCACGGTGTGCGACGATGACTTCTCGATCCATGCCGCCCA TGTGGTCTGCCGGCAAGTGGGCTATGTAGAGGCCAAGTCCTGGGCTGCCAGCTCCTCCTACGGTCCAGGC GAAGGCCCCATCTGGTTGGACAATATCTACTGTACTGGCAAAGAGTCGACCCTGGCATCTTGCTCCTCCA ATGGCTGGGGTGTCACTGACTGCAAGCACACTGAAGACGTTGGAGTGGTGTGTAGTGAGAAAAGAATTCC TGGCTTCAAATTTGACAATTCGTTGATCAACCAAATAGAGAGCCTAAATATACAGGTGGAAGACATCCGG ATTCGGCCCATCCTTTCTGCCTTTCGCCATCGCAAGCCTGTGACAGAGGGCTACGTGGAGGTGAAGGAGG GCAAGGCTTGGAAGCAGATCTGCAACAAACACTGGACAGCCAAGAATTCCCACGTGGTCTGTGGCATGTT CGGCTTCCCTGCAGAGAAGACCTACAACCCCAAAGCCTATAAAACCTTTGCCTCGCGGAGGAAGCTGCGT TACTGGAAGTTTTCTATGAACTGCACGGGCACTGAAGCGCATATCTCCAGCTGCAAGCTGGGCCCTTCCG TGACCCGGGACCCTGTGAAGAACGCCACCTGTGAGAACGGGCAGCCAGCTGTGGTCAGTTGTGTGCCTAG CCAGATCTTCAGCCCCGATGGACCCTCAAGGTTCCGGAAAGCCTACAAGCCAGAGCAACCCTTGGTGCGC CTGAGAGGTGGAGCCCAGGTCGGGGAGGGCCGAGTGGAGGTGCTGAAGAATGGAGAATGGGGAACCATCT GCGATGACAAGTGGGACCTGGTATCTGCCAGTGTGGTCTGCCGAGAGCTGGGCTTTGGGACCGCTAAAGA GGCCATCACAGGCTCCAGACTAGGGCAAGGGATGGCCGAGTGGAGGTGCTGAAGAATGGAGAATGGGGAA CCATCTGCGATGACAAGTGGGACCTGGTATCTGCCAGTGTGGTCTGCCGAGAGCTGGGCTTTGGGACCGC TAAAGAGGCCATCACAGGCTCCAGACTAGGGCAAGGGATTGGGCCCATCCATCTCAATGAAGTCCAGTGC ACAGGGACTGAGAAGTCCATCATAGACTGCAAATTCAACACAGAGTCTCAAGGCTGCAACCATGAAGAAG ATGCCGGGGTGCGATGCAACATCCCCATCATGGGTTTCCAGAAAAAGGTGCGCCTGAATGGAGGCCGCAA TCCTTATGAGGGCCGAGTGGAGGTGCTAACAGAGAGAAATGGGTCCCTTGTTTGGGGGACTGTATGCGGC CAGAACTGGGGCATTGTGGAAGCCATGGTGGTCTGCCGGCAGCTAGGCCTGGGCTTTGCCAGCAATGCCT TTCAGGAGACCTGGTACTGGCATGGAAATATCTTCGCCAACAACGTGGTCATGAGTGGAGTGAAGTGCTC AGGAACGGAGCTGTCCCTAGCACACTGCCGCCATGACGAGGAGGTGGCCTGCCCCGAGGGCGGGGTGCGG TTTGGTGCTGGAGTCGCCTGCTCGGAAACTGCACCTGACCTGGTGCTTAATGCTGAGATTGTCCAGCAGA CTGCCTACCTGGAGGACAGGCCCATGTCCTTGCTGCAGTGTGCCATGGAGGAGAACTGCCTCTCCGCCTC CGCTGTGCACACCGACCCCACCAGAGGCCACCGGCGCCTTTTACGCTTCTCCTCCCAGATCCACAACAAT GGCCAGTCTGACTTCCGCCCCAAGAATGGCCGCCATGCGTGGATTTGGCACGACTGCCACAGGCACTACC ACAGCATGGAAGTCTTCACTTACTATGACCTGCTGAGCCTCAACGGCACCAAGGTGGCTGAGGGCCACAA GGCCAGCTTCTGCCTGGAGGACACTGAGTGTGAGGGAGACATTCAGAAGAGTTACGAGTGTGCCAACTTT GGAGAACAAGGCATCACCATGGGCTGCTGGGACATGTACCGTCATGACATTGACTGCCAGTGGATAGACA TCACCGATGTGCCCCCTGGAGACTACCTGTTCCAGGTTGTCATTAACCCCAACTATGAAGTGCCAGAATC AGATTTCTCTAACAACATCATGAAGTGCAGGAGCCGCTATGATGGCTACCGCATCTGGATGTACAACTGT CACGTAGGTGGAGCCTTCAGTGAGGAGACAGAACAGAAGTTCGAACACTTCAGTGGACTTCTAAATAACC AGCTCTCTGTACAGTAAAGAAGATCCTGGGCCAGGCATGATGGCTCATGCCTGTAATCCCTGCACTCATG CTGAGGCAGGAGGATTGCCACAAGATTTCCACTCTGGACATTAAACCAAGCTTCAGTTTCAAAAGAAATG AATGAAAGAAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGAAAGGGGAAAGGGA AAGGGAAGAAAAATGACTTAATGGTCACTTACTGACTCCTGGGGGAATACTGATTACCACCTCTTTTCTA GCCAGATCCAGCTGAGAAGAAAGGTGCTCATTCACTCCCCAGACACTGCCGTGTGTCCCTGTCCTGAGGC CTTAGGGGCAGGGCTCGGGCACATGGCCATGGAAACTTGATGACAAGCTTAGAGCAGCTTATCCCATCCG AGCTTTGGCATGTCCCAAGTGTGACATCATCTGTGCTCTGCACAGAGGGGCCGTTTTCTTCTGGGAACAC AGCAGGCATGAACTCAGCAACTGCAGAGGTGATCGGGCTGAACTCCGTTTTTCCCCTTCTTAGGTCATTT CTGGAAAACTTGAATATCAAGACCTCTGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30605240 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30605240 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12446 same embryo
	EMAGE:12449 same embryo
	EMAGE:12448 same embryo
	EMAGE:12445 same embryo
	EMAGE:12447 same embryo
	EurExpress:euxassay_011221 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source