EMAGE mouse gene expression spatial database

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EMAGE:12445

Ift122 intraflagellar transport 122 homolog (Chlamydomonas) ( MGI:1932386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011142. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011142_01	euxassay_011142_02	euxassay_011142_03	euxassay_011142_04

euxassay_011142_05	euxassay_011142_06	euxassay_011142_07	euxassay_011142_08	euxassay_011142_09

euxassay_011142_10	euxassay_011142_11	euxassay_011142_12	euxassay_011142_13	euxassay_011142_14

euxassay_011142_15	euxassay_011142_16	euxassay_011142_17	euxassay_011142_18	euxassay_011142_19

euxassay_011142_20	euxassay_011142_21	euxassay_011142_22	euxassay_011142_23	euxassay_011142_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12445_wholemount_strong.wlz

12445_wholemount_moderate.wlz

12445_wholemount_weak.wlz

12445_wholemount_possible.wlz

12445_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12445_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

moderate

regional

moderate expression: see section 12 13 14 15 16 17

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 17 18 19 20 21 22

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 16 18 19 20 21 22 23 24

dorsal root ganglion

weak

regional

weak expression: see section 11 12 13 14 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 16 17 18 19 20 21

metanephros

weak

regional

weak expression: see section 08 09 10 11 12 13 20 21 22 23 24

testis

moderate

regional

moderate expression: see section 06 07 08 09 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9481
Entity Detected:	Ift122, intraflagellar transport 122 homolog (Chlamydomonas) ( MGI:1932386)
Sequence:	sense strand is shown >T9481 GAAAGAAGCCTGTTGCTGCCGAGGCTGGGAGGAGGGGCCCGAAGCGTGAGGGAAACGGCGATGAGGGCCG TGTTGACGTGGAGAGACAAAGCGGAGCAGTGTATCTACGACCTCGCATTTAAGCCTGATGGAACACAACT GATTCTGGCTGCAGGAAACAGATTGCTGGTTTATGACACCTCTGATGGCACCTTACTTCAGCCCCTGAAG GGACACAAAGACACCGTGTACTGTGTGGCGTATGCAAAGGATGGCAAGCGCTTTGCATCTGGATCTGCTG ACAAGAGCATCATTATCTGGACCTCAAAACTTGAAGGCATTCTGAAGTACACGCACAACGACTCTATTCA GTGTGTCTCCTACAATCCTGTTACCCACCAGCTGGCATCTTGTTCCTCCAGTGACTTTGGGTTATGGTCT CCTGAGCAGAAGTCTGTGTCCAAGCACAAATCCAGCAGCAAGATCACGTGCTGCAGCTGGACAAACGATG GTCAGTACCTGGCTCTGGGCATGGCCAATGGCATCATCAGTATACGGAACAAGAATGGTGAAGAGAAAGT GAAGATCGAGCGCCCTGGGGGCTCCCTCTCCCCAATATGGTCCATCTGCTGGAACCCTTCAAGAGAGGAA CATAATGACATCCTGGCTGTAGCTGACTGGGGACAGAAACTTTCCTTCTACCAGCTGAGTGGAAAACAGA TTGGGAAGGATCGGCCACTGAACTTTGACCCCTGTTGCATCAGCTACTTCACTAAAGGAGAGTACATTTT GGTGGGAGGTTCAGACAAGCAGGTATCTCTTTTCACCAAGGATGGAGTGCGACTGGGGACCGTTGGGGAG CAGAACTCCTGGGTGTGGACCTGTAGAGTGAAACCTGACTCCAATTATGTGGTGGTCGGCTGCCAGGATG GCACCATCTCCTTCTACCAGCTCATCTTCAGCACAGTCCATGGGCTCTACAAAGATCGGTATGCCTACAG GGACAGCATGACAGACGTCATCGTGCAGCACCTCATCACAGAGCAGAAAGTTAGGATTAAGTGCAGAGAG CTTGTCAAGAAAATTGCCATCTATAAAAATCGATTAGCTATCCAGCTGCCAGAGAAGATCCTCATCTACG AGCTGTACTCTGAAGACTCGACAGACATGCACTACCGGGTAAAGGAGAAAATTGTCAAGAAGTTTGAGTG CAACCTGCTGGTGGTGTGTGCTGACCACATCATCCTGTGCCAGGAGAAACGGCTACAGTGCCTGTCCTTC AGTGGAGTGAAGGAAAGGGAATGGCAGATGGAATCTCTCATCCGCTACATCAAGGTGATCGGTGGCCCTG CTGGAAGAGAAGGTCTGTTGGTAGGCCTGAAAAACGGCCAGATCCTGAAGATCTTTGTGGACAATCTTTT TGCTATTGTCCTGCTGAAGCAGGCCACAGCCGTGCGCTGCCTGGACATGAGTGCCTCCCGGAACAAGCTG GCTGTGGTGGATGAGAATGACACGTGCCTGGTGTACGACATCCACACGAAGGAACTGCTTTTCCAGGAAC CGAATGCTAACAGCGTGGCCTGGAACACGCAGTGTGAGGACATGCTCTGCTTCTCTGGAGGAGGCTACCT CAACATCAAGGCCAGCACCTTCCCAGTGCACCAGCAGAAGCTGCAGGGCTTTGTGGTTGGCTACAACGGC TCCAAGATCTTCTGCCTTCATGTCTTCTCTATGTCTGCCGTGGAGGTGCCACAGTCCGCTCCAATGTACC AGTACCTCGATAGGAAGATGTTCAAAGAAGCATATCAGATCGCTTGCTTGGGAGTGACAGACGCTGATTG GCGTGAACTGGCCATGGAAGCTCTAGAAGGACTAGAGTTTGAGACGGCAAGGAAGGCCTTCACCAGAGTC CAGGACCTTCGATACCTAGAACTGATCAGCAGTATTGAGGAGAGGAAGAAGAGGGGAGAGACCAACAATG ACCTGTTTCTGGCAGATGTGTTTTCCTACCAGGGGAAGTTTCATGAAGCCGCCAAACTATACAAGAGGAG CGGGCATGAGAACCTCGCTCTCGACATGTATACTGACCTCTGCATGTTTGAGTACGCCAAGGATTTCCTT GGATCTGGAGACCCTAAAGAAACGAAGATGCTCATCACCAAGCAGGCCGACTGGGCCAGAAACATCAATG AGCCCAAAGCTGCAGTGGAGATGTACATCTCAGCAGGGGAGCACGCCAAGGCCATAGAGATCAGCGGCAG CCATGGCTGGGTGGACATGTTGATAGACATTGCTCGGAAGCTGGACAAGGCTGAACGGGAGCCCCTGCTC ATGTGTGCTTGCTACTTTAAGAAGCTGGACAGTCCTGGCTATGCTGCTGAGACCTACCTGAAGATCGGGG ACCTGAAGTCTCTGGTGCAGCTGTATGTGGACACCAAGCGCTGGGATGAGGCCTTTGCGTTAGGTGAGAA GCACCCGGAGTTCAAGGATGACGTCTACGTGCCCTACGCGCAGTGGCTGGCGGAGAACGACCGCTTTGAG GAAGCCCAGAAAGCCTTCCACAAGGCCGGGAGGCAGGGAGAAGCAGTCCGGGTGCTGGAGCAGCTCACGC ACAATGCTGTGGTGGAGAGCAGGTTTAACGACGCTGCCTATTATTACTGGATGCTGTCCATGCAGTGCCT CGACATGGCTCAAGATCCTGCCCAGAAGGACGCGATGCTGGACAAGTTTCACCACTTCCAACACCTGGCT GAGCTGTACCATGGCTATCAAACCATTCACCGATACACGGAGGAGCCATTCAGCTTTGACCTCCCTGAAA CCCTCTTCAACATCTCCAAGTTCTTGCTGCACAGCCTGACCAAGGCCACCCCCTTGGGTATCTCCAAAGT GAATACACTCTTCACACTGGCTAAGCAGAGCAAGGCTCTGGGTGCTTACAAGCTGGCCCGGCATGCCTAT GACAAGCTACGGGGGCTGCAGATCCCCGCCAGGATCCAGAAGTCCATTGAGCTGGGCACCCTGACCATCC GCTCCAAGCCCTTCCATGACAGTGAGGAGCTGGTACCCTTGTGCTACCGCTGTTCTACCAACAACCCGCT GCTGAACAACCTTGGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTTCTCCGCCTCTTCCTATGAG GTGCTGCACCTGGTGGAATTCTACCTGGAAGAGGGGATCACTGACGAAGAAGCTGTTGCCCTCATCGATC TGGAGGCACCAAGACACAAGAGGGAGGGCAAGTGGCGAGAGACCTCAAGCAACAATTCCCAGACGTTGAA GCTGGACGAGACCATGGACTCCATAGGAGAAGATGACCCATTTACAGCCAAGCTGAGCTTTGAGCAAGGT AGCTCTGAGTTTGTGCCTGTGGTGGTGAACCGGTCAGTACTACGCTCCATGAGCCGAAGAGATGTTCTCA TCAAGCGCTGGCCCCCGCCGCTGCAATGGCAATATTTCCGCTCGCTGTTGCCTGATGCCTCCATCACCAT GTGCCCCTCCTGCTTTCAGATGTTCCATTCAGAGGACTACGAACTGCTTGTGCTCCAGCACGCCTGCTGC CCTTACTGCCGAAGGCGTATCGACGACACTGGCCCGTGAGCAGCGACAGCCTCTGGGACCACGGCTCCCA TGGTCTCCTTGCTGTCCCCAGACTGTTGGGAGAAGGAATTAAACCCTCAGAACACGTCTCCTGCCCAGAT GAAGTTTGTATGTTGCAAAGGGGGGGGGGGCTGCCTTGTGTAACCACAGATTTCCTATTTAGGCATTTCA TTCCTTGTAAATAGCACCAGGAGACGGGGAAGAGAACACACACATATTTTCTAAGAAAAAAAAAAAATCT GTTACTTTTAGAAGGGCTCATGTGTTCATAGCAGCCTGCTGGAGTCCGTAGATCCGTCAGACCATTACCG TGCACACGTTGCCAACAGATTGCCTCAATAACGACAAATAAAAGGGCTACTCGTTTTCATCAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30355922 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30355922 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12446 same embryo
	EMAGE:12449 same embryo
	EMAGE:12448 same embryo
	EMAGE:12447 same embryo
	EMAGE:12444 same embryo
	EurExpress:euxassay_011142 same experiment
	MGI:4825529 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source