EMAGE mouse gene expression spatial database

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EMAGE:12449

Ttc26 tetratricopeptide repeat domain 26 ( MGI:2444853)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011112. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011112_01	euxassay_011112_02	euxassay_011112_03	euxassay_011112_04

euxassay_011112_05	euxassay_011112_06	euxassay_011112_07	euxassay_011112_08	euxassay_011112_09

euxassay_011112_10	euxassay_011112_11	euxassay_011112_12	euxassay_011112_13	euxassay_011112_14

euxassay_011112_15	euxassay_011112_16	euxassay_011112_17	euxassay_011112_18	euxassay_011112_19

euxassay_011112_20	euxassay_011112_21	euxassay_011112_22	euxassay_011112_23	euxassay_011112_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12449_wholemount_strong.wlz

12449_wholemount_moderate.wlz

12449_wholemount_weak.wlz

12449_wholemount_possible.wlz

12449_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12449_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9324
Entity Detected:	Ttc26, tetratricopeptide repeat domain 26 ( MGI:2444853)
Sequence:	sense strand is shown >T9324 TGGCAACTCGCCGAAGCACAGCCTTTGCAGACTATCTGCAGCGGGGGCTCACAGGACCGACGCCAAGATG ATGCTTTCAAGGGCCAAACCCGCTGTGGGCGGGGAGTCACCGCACACTGACAAAAGAAAGAAGAAAGGGA GGAAGATTCCAAAACTAGAGGATCTACTTTCGCAGAGAGATTTCACTGGCGCCATTACCCTATTGGAGTT CAAGCGCCACGTTGGGGAGCAAGAAGACGACACTAACTTATGGATTGGATACTGTGCTTTTCACCTGGGT GACTACAAGAGAGCTTTGGAGGAATATGAAAATGCAACGAAGGAGGAAAATTGCAATCCAGAAGTGTGGG TGAACTTGGCCTGCACCTACTTTTTTCTTGGGATGTATAAACAAGCAGAAGCTGCTGGGTTTAAAGCTCC AAAAAGCCGGCTCCAAAACCGCCTACTGTTCCATCTGGCTCACAAGTTTAATGATGAGAAGAAATTAATG AACTTTCATCAGAATCTTCAGGATATCAAGGAAGACCAGCTTAGTTTGGCCTCAATCCACTACATGCGAT CCCATTACCAAGAAGCTATTGATATATACAAGCGGATCCTGCTGGACAACAGGGAATACCTGGCCCTCAA TGTGTATGTGGCCCTCTGCTACTACAAACTGGATTACTATGATGTGTCTCAAGAAGTCCTGGCTGTTTAC CTTCAGCAGATTCCTGACAGTACCATCGCCCTCAACCTTAAGGCCTGCAATCACTTTCGCCTTTATAACG GCAAAGCAGCTGAGGCAGAGCTCAAAAGCTTGATGGACAATGCATCTTCACCCTTTGAATTTGCTAAAGA ACTCATCAGGCACAATCTGGTTGTTTTCCGAGGAGGCGAAGGGGCTTTGCAGGTTTTACCTCCCCTGGTT GATGTCATTCCTGAAGCTAGGCTCAACCTAGTGATTTACTACCTTCGTCAAGATGATGTGCAGGAAGCCT ATAATTTAATCAAGGATTTGGAACCCACTACACCTCAGGAGTATATCCTCAAGGGAGTTGTCAATGCAGC CCTTGGCCAGGAAATGGGTTCAAGGGATCATATGAAAATTGCCCAGCAGTTTTTCCAGTTGGTAGGAGGA TCAGCTAGTGAATGTGATACAATACCAGGAAGGCAGTGCATGGCTTCCTGTTTCTTCCTACTTAAACAAT TTGATGATGTCTTGATTTACCTCAACTCTTTTAAGAGTTACTTCTATAATGATGACATCTTTAACTTTAA TTATGCCCAAGCCAAAGCTGCAACAGGCAATACCAGTGAGGGTGAAGAGGTTTTCCTCTTGATCCAAAGT GAGAAACTGAAAAATGACTACATTTACCTTAGCTGGTTAGCTCGCTGCTATATTATGAATAAGAAACCAA GACTAGCTTGGGAGCTTTATCTCAAGATGGAAACCTCTGGCGAGTCCTTCAGCCTCTTACAGCTCATTGC CAATGACTGTTACAAGATGGGCCAGTTCTACTATTCAGCCAAAGCTTTTGATGTCTTAGAAAGACTGGAT CCCAACCCTGAGTACTGGGAAGGCAAGAGGGGTGCCTGTGTGGGCATCTTCCAGATGATCTTAGCTGGGA GAGAACCCAAAGAGACCCTCCGAGAAGTGCTGCATTTACTGAGAAGCACAGGAAACACCCAAGTGGAGTA TATCATCAGGATCATGAAGAAGTGGGCCAAGGAGAATAGAGTGCCCATCTAAGCAGCCAAACCCTAGGCC AGGACCAGCTTCCTCTCACGTCAAATTGGAACTGTTTTCTTGCACTTGAATATGCAATCCAGGACCCTGC TTGATTGACATTTCTTTCCTTGCTCTTAGAGCATTAGGATGAGTGGGTGGTATGTGTGGACTTAGGCATC AGGCAACAGGAATCTCCCTGGCCTGCTGACACCCAATGTAATCTACTGGTGCCTTTCTTCCATAATCATT CTGAACACTATTCTGTAATTTACTGGCTTTAGGAATAGATACTAGTTTTGTTTTTCTTTCTCTGTTAGCA TTTCAGGACATATCTTTTTTCTTCTTCTTTTTTTTTTTTTTCTGAGACAGGGTTTCTCTGTATAAGCCCT GCCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCACCTGCCTCTGCCTCCT GAGTGCTGGGATTAAAGGCGTGTGCCACCATGCCCGGCAGGACATTTTCTCCTACCTGCAGTAACAAGTA CCTTTATACTAGAGAATTGCCATCATTTGATCATATTCCATCATTTTGTACATTAGCCCATCCATGACTA CATCTCTGACTCCTGGGAGACGTCTTTCTGAGCTGTGTGATTCTGAGGATGGCCATGAGGCCCTCTACCA AACAGCAGTCAGTAGTAGTTAGTTAGCTTATGAGACCACTCTCTGTGAGTAGCTTGTTACGTTGAGACAA GCTGAGTATATGGTTTTAAAAAGGTGAGTGAAAGGAAAAACTTGGCATCTCTGAAGGTGTGTAAGTCTCA GAGTTAGGTCTCCCTTGTATTTTAGGAAACAAAGCCAGGGCAGGGTTCTTACTATCTTCTGAATGAACAG CAATTTTGAACATAGGACTGGGAAAGTTTTCATGAAATATATATATATGTATATTTTTTCTGAGACAGGG TTTCTCTGTGTAGCCTTGGCTGTCATGGAACTCACTCTGTAGACTAGGCTGGCCTTGAACTCGGAGATCC GCCTGCCTCTGCCTCCCGAGTGCTCGTATCAAAGATGTGTGCACCACTGCTCAGCATACGTACCTGTTTA TATTGCGCACACATGAACACATCGTAGGGATTTATTGATACAAGTATAAAGCAGGTCCTTTGAAGAGACC TCCTAATGGCTAGCCTCTCCATCATATCTCAGCCTCGTGTCTCTGAGCTGTCATCACATAAACTTCTTTT TTCTACAACAAAGTATTGTCAGGCTTCCATCCTACTTTCAGAAAACAGACATACATCTTTTTGTGTATCA GATTACCTGCTTGTTTGTATGCTCTGTAATTTTCTTCTGTAGGTCTGAGGTACACAAGCTAGAACATACA GACCCATCCTCATAGAGCAGGGACTGCACAGAGGAGGAGGTGGAGGAATTTTTAGAAACACCATTAATTC TTCTTCCTTCCCAGCCTCCTGCTGCAGATGGCAGATGGGAGTTCTGCACAGAAAGGAGAGGGAAACCTGT ACAGAGTAAAACAGCTGAAGCAACAGGACCAGAGGCTGAACACAGCCTTCATCTCTAGCCAGTCCCAAGG TGTTCCTCAGCTCTGTATCTCTGAGTCCTGGGACAAGTCTTCCCGAGAAGATGGCCAGTGTGCCATCTTT TACATTAGGATCAGATATGATTGGGCCAAAGCACAAAACACAGTACTGGTCTAACAGAACTGCAGGAAAC AAAACTGCACCATTTCCTTATGCCAGAGCTTCTTGTCTTGTTCTGGTGTTCCCTCTTTTTCAGTAGCACC TGGTTCTCTTTGCCAAAGGTACTAGGTGGATCAGATATGACTAAACCTGCTATAGTTCTGTGAATCATTC TTATGAAACTTATTTTGCTTTAAATACTTGGAAGCAAAAGAGTGGGATGCAGATCAACTCCTGGGAGCTG TTGACATCTTCAGTGACACACTGTTCAGTTCCAGCTTCCTGAAAGACACTCATTCACAGTCATGGACTTC CCAGTGAGACATTGTGAGTTTACTACTTAGTAGTCTCTGACATTTTCCTTGAGAGAAGACGGTCTGTCCC CAACAGCAGAATTTTAGATTACTGTTAAAAGTTTTTAATCTACAGGATTGAGACAGAAACATGAAGGCTT GTTAAAGAGAAGCACATTCAATTCTGTTAGGAGGGATAATTGTTTCTGATTGTCTTGACAGGGCATCAAG TTCGTACTGTGTTCTCTCACAGATGACAGCCTGGAAAAGAAGGGCTTGGCTGGGGAATGTGTAATTTATA CAGTCTATCATAGCAATGTACTATTTAAAAATAGTCTGTAGTGTATACTTAAAAATGGAAAAAACACTTT TACAACTATTTTTGTACAACAAAAGAATCACATACCACTGTACTATATTTAAATTAAAATTTATTTTTTG GTTTTTGTCAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6848602 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6848602 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12446 same embryo
	EMAGE:12448 same embryo
	EMAGE:12445 same embryo
	EMAGE:12447 same embryo
	EMAGE:12444 same embryo
	EurExpress:euxassay_011112 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source