EMAGE mouse gene expression spatial database

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EMAGE:12456

Myl3 myosin, light polypeptide 3 ( MGI:97268)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011246. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011246_01	euxassay_011246_02	euxassay_011246_03	euxassay_011246_04

euxassay_011246_05	euxassay_011246_06	euxassay_011246_07	euxassay_011246_08	euxassay_011246_09

euxassay_011246_10	euxassay_011246_11	euxassay_011246_12	euxassay_011246_13	euxassay_011246_14

euxassay_011246_15	euxassay_011246_16	euxassay_011246_17	euxassay_011246_18	euxassay_011246_19

euxassay_011246_20	euxassay_011246_21	euxassay_011246_22	euxassay_011246_23	euxassay_011246_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12456_wholemount_strong.wlz

12456_wholemount_moderate.wlz

12456_wholemount_weak.wlz

12456_wholemount_possible.wlz

12456_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12456_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 22 23 24

upper leg muscle

strong

regional

strong expression: see section 05 06 07 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04

hand

strong

regional

strong expression: see section 04

foot

strong

regional

strong expression: see section 04 05 06 22 23

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

extrinsic ocular muscle

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 19 20 21 22 23 24

heart atrium

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

heart ventricle

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15 16 17

tail mesenchyme

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9966
Entity Detected:	Myl3, myosin, light polypeptide 3 ( MGI:97268)
Sequence:	sense strand is shown >T9966 AGTAGGACTCACCTATCTTACTCTCTTCTCCTTTCCTTCTTCCCTCCTCTTTGTGATTACAGCTCCAATG GCCCCCAAAAAGCCAGAGCCCAAGAAGGATGATGCCAAAGCGGCTGCCCCCAAAGCAGCTCCAGCTCCTG CGGCTGCACCTGCAGCTGCACCTGCGGCTGCACCAGAGCCTGAGCGCCCCAAGGAAGCCGAGTTTGATGC CTCCAAGATTAAGATCGAGTTCACACCTGAACAGATTGAAGAGTTCAAGGAGGCCTTCCTGCTGTTCGAC CGCACACCCAAGGGCGAGATGAAGATCACATACGGGCAGTGTGGGGATGTCCTGCGGGCTCTGGGTCAGA ATCCTACCCAGGCAGAGGTGCTCCGTGTCCTGGGGAAGCCAAAACAGGAAGAGCTCAATTCCAAGATGAT GGATTTTGAGACGTTCCTGCCTATGCTCCAGCACATCTCCAAGAACAAGGACACTGGCACGTACGAGGAC TTCGTGGAGGGGCTGCGGGTCTTCGACAAGGAGGGCAACGGCACGGTCATGGGTGCAGAGCTCCGACACG TGCTGGCCACGCTGGGTGAGAGACTGACAGAAGATGAGGTAGAGAAACTGATGGCTGGTCAAGAGGACTC CAACGGCTGCATCAACTATGAAGCGTTTGTGAAGCATATCATGGCGAGCTGAGCCTCTCAGGAAGCCCAG GGCAGGCCAAGCATCTCCCAACCATGATGCTGACACCATGTCTGGAGCTTCGGGAAGGAGTGGTTCGGAC TCCTGCAGGTTGTCCTGGATCACTCTGTGTGTGGTAGTCTTTCCTGGCCCCTGCTGGGATGGGCTTACCT ACCTGTGCTTGCCTTTCTGTATCCACCCTGTGGCCTTATGAATAAATGGTTTCCTTCCTTCCTTGAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30297023 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30297023 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12454 same embryo
	EMAGE:12455 same embryo
	EMAGE:12457 same embryo
	EurExpress:euxassay_011246 same experiment
	MGI:4826549 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source