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EMAGE:1246

Nefm neurofilament, medium polypeptide ( MGI:97314)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:1246
Fig 7A Niederreither et al, 2000 [PMID:10654602] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: b1= first branchial arch, mb = midbrain, gV/gVII = cranial nerve ganglia.
Expression Pattern Description
Spatial Annotation:
EMAGE:1246Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1246_wholemount_strong_3D_1.wlz
1246_wholemount_moderate_3D_1.wlz
1246_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1246_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regionalExpression is in nerves
future hindbrain
detected detected
single cellThere is expression in a few nerve fibres
facio-acoustic vii-viii preganglion complex
detected detected
Expression is in nerves
trigeminal v preganglion
detected detected
Expression is in nerves
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1330766
Entity Detected:Nefm, neurofilament, medium polypeptide ( MGI:97314)
Notes:The anti-NEF3 antibody used in this study by Niederreither et al, 2000 [PMID:10654602] is described as "2H3 neurofilament specific antibody (Developmental Studies Hybridoma Bank)". Details below are taken from the Developmental Studies Hybridoma Bank online catalogue. This antibody detects a 165 kDa form of NEF3.
Antibody Type:monoclonal
Raised In:mouse
Supplier:Developmental Studies Hybridoma Bank
Catalogue Number:2H3
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Niederreither et al, 2000 [PMID:10654602] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10654602] Niederreither K, Vermot J, Schuhbaur B, Chambon P, Dolle P 2000 Retinoic acid synthesis and hindbrain patterning in the mouse embryo. Development (127):75-85
Links:MGI:1350665 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI