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EMAGE:1247

Nefm neurofilament, medium polypeptide ( MGI:97314)
TS18 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:1247
Fig 4A Xu et al, 1998 [PMID:9486805] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1247Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1247_wholemount_strong_3D_1.wlz
1247_wholemount_moderate_3D_1.wlz
1247_wholemount_weak_3D_1.wlz
1247_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1247_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future spinal cord
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1330766
Entity Detected:Nefm, neurofilament, medium polypeptide ( MGI:97314)
Notes:The anti-NEF3 antibody used in this study by Xu et al, 1998 [PMID:9486805] is described as "2H3, reactive to neurofilament protein (Developmental Studies Hybridoma Bank, Johns Hopkins University, Baltimore, MD)". The extra information below has been taken from the Developmental Studies Hybridoma Bank online catalogue.
Antibody Type:monoclonal
Raised In:mouse
Supplier:Developmental Studies Hybridoma Bank
Catalogue Number:2H3
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Secondary Antibody:goat anti-mouse IgG
Labelled with:horse radish peroxidase
Visualisation method:Vector(TM)SG
General Information
Authors:Xu et al, 1998 [PMID:9486805] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9486805] Xu W, Baribault H, Adamson ED 1998 Vinculin knockout results in heart and brain defects during embryonic development. Development (125):327-37
Links:MGI:1338932 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI