EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12476

4930599N23Rik RIKEN cDNA 4930599N23 gene ( MGI:1922629)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011235. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011235_01	euxassay_011235_02	euxassay_011235_03	euxassay_011235_04

euxassay_011235_05	euxassay_011235_06	euxassay_011235_07	euxassay_011235_08	euxassay_011235_09

euxassay_011235_10	euxassay_011235_11	euxassay_011235_12	euxassay_011235_13	euxassay_011235_14

euxassay_011235_15	euxassay_011235_16	euxassay_011235_17	euxassay_011235_18	euxassay_011235_19

euxassay_011235_20	euxassay_011235_21	euxassay_011235_22	euxassay_011235_23	euxassay_011235_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12476_wholemount_strong.wlz

12476_wholemount_moderate.wlz

12476_wholemount_weak.wlz

12476_wholemount_possible.wlz

12476_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12476_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 18 weak expression: see section 07

trigeminal v ganglion

moderate

single cell

moderate expression: see section 17 18 19 weak expression: see section 03 04 05 06 07 08 09

dorsal root ganglion

moderate

single cell

moderate expression: see section 08 09 10 18 19 weak expression: see section 12 13 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37393
Entity Detected:	4930599N23Rik, RIKEN cDNA 4930599N23 gene ( MGI:1922629)
Sequence:	sense strand is shown >T37393 GTTCAGTCAGCAACCTCGATCTCGGAAAAGCCGAGCGAAGTTTCCAGACGGCGCCGGCCGGTTGCGGCCG GTTGGTGCGTCGGCCGGCGCGCTCAGGGGGGCTCAGAAGTCGCCCGAGCCAAAGCGGGCTTTAGCAGGCG GGCCTCGGAAGCGGGGACGACGGACCCGGTCCCACCGCACCCCGGAACAAACGGAAACAGTCGCTCCGAA TTGCCAGACACTCCGCTTTTGTAGCGGATCCCGCATTTCCAGAAGGAAAGCGTAGGTACGTCCTTTAAAG GGGGCCGATCCTCCCTCCCTCGCCCCTTCTGAGTAAAGGTGATGCACCTAGTTCGATTCCTCACCGGAAA GAACTCTCCTGTGTGTTACCCTGGGAGTTAAACGTTCTCGGGGTAAGTAAACGGGGGTGTGTGGGGGTAT ACCTATTAAAATACGACTTTTGGCACCCCCATTCCTTCCTCCAGGAGTCTCCCGGGAATAAAAGCGTTAG TAAGTCATGTTCACAGGAGAGGACGGAGTACTGGAAACAGTAAGACGGTGATTCAGACAACAAGAGTTTA ACAAGCATGTTAATTCTCTGGGTTTGACCTAGAAGGACATTAGTATGTTGTGAGAAAAGATTGTAATCAA GTAGTATTCCATGGGGCTGATCGGAGGTTAAGCGGCCGCACTGCTCTGGAAGAGGACCTCGGCCGGGTTC CCAGCACCCACATAGCAGCTCACAACTGCCTGTAACCCTGGTTCCAGGAGGGATCTGACAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68322. Forward Primer - name:068322_F_cDNA_4930599N23Rik, sequence:GTTCAGTCAGCAACCTCGATCT; Reverse Primer - name:068322_N_SP6_cDNA_4930599N23Rik, sequence:GTGTCAGATCCCTCCTGGAAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12471 same embryo
	EMAGE:12474 same embryo
	EMAGE:12475 same embryo
	EMAGE:12472 same embryo
	EMAGE:12470 same embryo
	EMAGE:12473 same embryo
	EurExpress:euxassay_011235 same experiment
	MGI:4822743 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source