EMAGE mouse gene expression spatial database

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EMAGE:12477

Snap29 synaptosomal-associated protein 29 ( MGI:1914724)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011249. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011249_01	euxassay_011249_02	euxassay_011249_03	euxassay_011249_04

euxassay_011249_05	euxassay_011249_06	euxassay_011249_07	euxassay_011249_08	euxassay_011249_09

euxassay_011249_10	euxassay_011249_11	euxassay_011249_12	euxassay_011249_13	euxassay_011249_14

euxassay_011249_15	euxassay_011249_16	euxassay_011249_17	euxassay_011249_18	euxassay_011249_19

euxassay_011249_20	euxassay_011249_21	euxassay_011249_22	euxassay_011249_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12477_wholemount_strong.wlz

12477_wholemount_moderate.wlz

12477_wholemount_weak.wlz

12477_wholemount_possible.wlz

12477_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12477_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 14 15

pons mantle layer

weak

regional

weak expression: see section 07 16

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 08

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 16 17

ventral grey horn

weak

regional

weak expression: see section 09 10 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 15 16

neural retina

weak

regional

weak expression: see section 01 02 03 22

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 13 14 15 16

vomeronasal organ

weak

regional

weak expression: see section 11 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1079
Entity Detected:	Snap29, synaptosomal-associated protein 29 ( MGI:1914724)
Sequence:	sense strand is shown >T1079 GTTGGCCTACTGGAAAGTAGTGCGCGCGGCGTCCACAGTGAATCCGCCCGCCGGCGCTGGGCTATCGACT CCTCGCTCAGCAACCCCTCCTGCTTCCTAGGTGCAGGCCGTCTCCCGCACCATGTCTGGCTATCCTAAAA GCTATAATCCTTTCGACGATGACGTGGAAGAGGAAGACACCCGGCCCGCGCCGTGGAAGGACGTCCGCGA CCTGCCTGACGGCCCCGACGCGCCCATTGACAGGCAGCAGTACCTGAGACAGGAGGTGTTGCGCAGGGCC GAGGCTACCGCTGCCAGTACCAGCAGGTCCTTGTCTCTCATGTATGAATCGGAGAAGATCGGAGTCGCCT CTTCCGAGGAGCTGGTCCGGCAGCGAGGAGTCCTAGAACACACAGAGAAGATGGTAGACAAGATGGATCA GGATTTGAAGATGAGCCAGAAACATATCAACAGCATTAAGAGTGTGTTTGGAGGATTTATCAACTACTTC AAATCCAAACCAGTAGAGCCTCCACCTGAGCAGAATGGCAGCATCGTCTCCCAGCCCAACAGCAGATTGA AAGAAGCCATAAATA
Notes:	The probe template was PCR amplified from IMAGE:2099924 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2099924 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12478 same embryo
	EMAGE:12480 same embryo
	EMAGE:12482 same embryo
	EMAGE:12479 same embryo
	EMAGE:12481 same embryo
	EurExpress:euxassay_011249 same experiment
	MGI:4828343 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source