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EMAGE:1248

Nes nestin ( MGI:101784)
TS20 (12.5 dpc)
immunohistochemistry

Data Images
EMAGE:1248 EMAGE:1248
Fig 1 Part 2Cc, Handler et al, 2000 [PMID:10821758] . Copyright: This image is from Development and is displayed with the permission fo the Company of Biologsists Ltd who owns the copyright. Fig 1 Part 2C, Handler et al, 2000 [PMID:10821758] . Copyright: This image is from Development and is displayed with the permission fo the Company of Biologsists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
The region shown in image 1 is indicated by the red square marked "a-d" in Image 2. Image annotations: lv - lateral ventricle.
Expression Pattern Description
Spatial Annotation:
EMAGE:1248EMAGE:1248Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1248_voxel_strong_3D_1.wlz
1248_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1248_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
detected detected
regionalExpression is in neuronal progenitors in the neuroepithelium
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1336115
Entity Detected:Nes, nestin ( MGI:101784)
Notes:The anti-NES antibody used in this study by Handler et al, 2000 [PMID:10821758] is described as "an anti-Nestin monoclonal antibody (1:1000, PharMingen)". Editors Note: The extra details included here are taken from the Pharmingen online catalogue (http://www.bdbiosciences.com).
Antibody Type:monoclonal
Raised In:mouse
Supplier:Pharmingen
Catalogue Number:556309
Specimen
Organism:mouse
Strain:(129S2/SvPas x C57BL/6) or (129S4/SvJae x C57BL/6)
Age:12.5 dpc
Theiler Stage:TS20
Mutations:Psen1tm1Shn/Psen1+
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Secondary Antibody:goat anti-mouse
Labelled with:rhodamine
Visualisation method:fluorescence
General Information
Authors:Handler et al, 2000 [PMID:10821758] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10821758] Handler M, Yang X, Shen J 2000 Presenilin-1 regulates neuronal differentiation during neurogenesis. Development (127):2593-606
Links:MGI:1859903 same experiment
 MGI:1857974 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI