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EMAGE:12493

Stau2 staufen (RNA binding protein) homolog 2 (Drosophila) ( MGI:1352508)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12493 EMAGE:12493 EMAGE:12493 EMAGE:12493 EMAGE:12493
"Pseudo-wholemount" of euxassay_011484. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011484_01 euxassay_011484_02 euxassay_011484_03 euxassay_011484_04
EMAGE:12493 EMAGE:12493 EMAGE:12493 EMAGE:12493 EMAGE:12493
euxassay_011484_05 euxassay_011484_06 euxassay_011484_07 euxassay_011484_08 euxassay_011484_09
EMAGE:12493 EMAGE:12493 EMAGE:12493 EMAGE:12493 EMAGE:12493
euxassay_011484_10 euxassay_011484_11 euxassay_011484_12 euxassay_011484_13 euxassay_011484_14
EMAGE:12493 EMAGE:12493 EMAGE:12493 EMAGE:12493 EMAGE:12493
euxassay_011484_15 euxassay_011484_16 euxassay_011484_17 euxassay_011484_18 euxassay_011484_19
EMAGE:12493 EMAGE:12493 EMAGE:12493 EMAGE:12493
euxassay_011484_20 euxassay_011484_21 euxassay_011484_22 euxassay_011484_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12493Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12493_wholemount_strong.wlz
12493_wholemount_moderate.wlz
12493_wholemount_weak.wlz
12493_wholemount_possible.wlz
12493_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12493_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 07 08 16 17 18
hypothalamus mantle layer
weak weak
homogeneousweak expression: see section 08 09 10 11 12 13 14 15
diencephalon lateral wall mantle layer
weak weak
homogeneousweak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
telencephalon mantle layer
weak weak
homogeneousweak expression: see section 01 02 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
medulla oblongata alar plate mantle layer
moderate moderate
homogeneousmoderate expression: see section 07 08 09 13 weak expression: see section 06 14 15 16 17 18
medulla oblongata basal plate mantle layer
moderate moderate
homogeneousmoderate expression: see section 07 08 09 10 12 13 weak expression: see section 11 14 15 16 17
rest of cerebellum mantle layer
weak weak
homogeneousweak expression: see section 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21
pons mantle layer
weak weak
homogeneousweak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18
midbrain mantle layer
weak weak
homogeneousweak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 05 19 20 weak expression: see section 04
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 17 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 05 06 07 16 17 18 19 20 21 22 weak expression: see section 04
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 08 weak expression: see section 16
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 07 17 18
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 08 weak expression: see section 16
spinal cord mantle layer
moderate moderate
homogeneousmoderate expression: see section 08 09 10 12 13 weak expression: see section 11 14 15 16
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 13 14 16 17 weak expression: see section 15
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 weak expression: see section 22 23
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 08 09 10 13 14 15 16 17
renal cortex
weak weak
regionalweak expression: see section 06 07 08 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30303
Entity Detected:Stau2, staufen (RNA binding protein) homolog 2 (Drosophila) ( MGI:1352508)
Sequence:sense strand is shown

>T30303
TCCAAAGCCATTCCCAAATTATAGAGCTAACTACAACTTCCGGGGCATGTACAATCAGAGGTATCATTGC
CCAATGCCCAAGATCTTTTATGTTCAGTTAACTGTAGGAAATAATGAATTCTTTGGTGAAGGGAAGACTC
GACAAGCTGCCAGACACAATGCTGCGATGAAAGCGCTTCAAGCCCTACAGAATGAGCCAATTCCAGAAAA
GTCTCCCCAGAATGGTGAATCAGGAAAAGAAATGGATGACGATAAAGATGCAAATAAATCTGAAATAAGC
TTAGTGTTTGAGATTGCGCTGAAGAGAAATATGCCTGTCAGTTTTGAGGTTATTAAAGAAAGTGGACCAC
CACATATGAAGAGTTTTGTAACTCGGGTGTCAGTGGGAGAATTTTCTGCAGAAGGAGAGGGAAATAGCAA
AAAACTCTCCAAGAAGCGTGCCGCAACCACTGTCTTACAGGAGCTTAAAAAACTCCCACCTCTTCCTGTG
GTAGAGAAGCCAAAACTATTTTTTAAAAAACGCCCTAAAACAATAGTAAAGGCTGGACCCGACTATGGTC
AAGGAATGAACCCCATTAGCCGCCTGGCTCAGATCCAGCAAGCCAGAAAGGAAAAGGAGCCTGATTACAT
CCTACTCTCAGAACGAGGAATGCCTCGCCGTCGAGAGTTCGTAATGCAGGTCAAGGTAGGCAATGAAGTT
GCGACTGGAACAGGACCCAATAAAAAGATAGCCAAAAAAAATGCTGCTGAAGCAATGCTGTTACAGCTTG
GCTATAAAGCGTCCACCAGTCTTCAGGATCCGCTCGACAAGACAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3670067), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 29938. Forward Primer - name:029938_F_IRAV77-80_I10_Stau2, sequence:TCCAAAGCCATTCCCAAA; Reverse Primer - name:029938_R_SP6_IRAV77-80_I10_Stau2, sequence:ACCTGTCTTGTCGAGCGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12494 same embryo
 EMAGE:12492 same embryo
 EurExpress:euxassay_011484 same experiment
 MGI:4828491 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS