EMAGE mouse gene expression spatial database

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EMAGE:12496

Ndn necdin ( MGI:97290)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011297. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011297_01	euxassay_011297_02	euxassay_011297_03	euxassay_011297_04

euxassay_011297_05	euxassay_011297_06	euxassay_011297_07	euxassay_011297_08	euxassay_011297_09

euxassay_011297_10	euxassay_011297_11	euxassay_011297_12	euxassay_011297_13	euxassay_011297_14

euxassay_011297_15	euxassay_011297_16	euxassay_011297_17	euxassay_011297_18	euxassay_011297_19

euxassay_011297_20	euxassay_011297_21	euxassay_011297_22	euxassay_011297_23	euxassay_011297_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12496_wholemount_strong.wlz

12496_wholemount_moderate.wlz

12496_wholemount_weak.wlz

12496_wholemount_possible.wlz

12496_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12496_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata floor plate

moderate

regional

moderate expression: see section 11 12 13 14

metencephalon floor plate

moderate

regional

moderate expression: see section 12

cornea epithelium

moderate

regional

moderate expression: see section 01 21 22 23 24

lens

moderate

regional

moderate expression: see section 24

esophagus

moderate

regional

moderate expression: see section 13

mandible

weak

regional

weak expression: see section 04 05 06 07 17 18 19 20

orbito-sphenoid

moderate

regional

moderate expression: see section 01 weak expression: see section 02 21 22 23

clavicle

weak

regional

weak expression: see section 06 07 08 09 10 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30148
Entity Detected:	Ndn, necdin ( MGI:97290)
Sequence:	sense strand is shown >T30148 CCCTAACTTTGCAGCCGAGGTCCCCGACTGTGAGATGCAGGACAGCGATGCCGTTCCGGTGGGGATCCCT CCTCCCGCTTCTCTGGCCGCTAACCTCGCAGGGCCACCGTGCGCTCCCGAAGGCCCTATGGCAGCCCAAC AGGCCTCGCCACCGCCCGAAGAACGGATAGAAGATGTTGACCCTAAAATCCTGCAGCAGGCCGCAGAGGA GGGCCGCGCCCACCAGCCCCAGAGTCCAGCCCGGCCGATCCCAGCACCGCCAGCCCCTGCCCAGCTGGTG CAGAAGGCGCACGAGCTCATGTGGTACGTGTTGGTGAAGGACCAGAAGAGGATGGTCCTCTGGTTTCCAG ACATGGTGAAAGAGGTCATGGGCAGCTACAAGAAATGGTGCAGAAGCATCCTCAGGCGCACCAGCGTCAT CCTCGCCAGAGTGTTCGGGCTGCACCTGAGGCTGACCAATCTCCACACCATGGAGTTTGCCCTGGTCAAA GCCCTCAGCCCAGAGGAGCTAGACAGGGTGGCGCTCAACAACCGTATGCCCATGACAGGCCTCCTGCTCA TGATCCTGAGCCTCATCTATGTGAAGGGCCGCGGGGCCAGAGAGGGTGCGGTCTGGAATGTGCTGCGCAT CCTGGGGCTGAGGCCCTGGAAGAAGCACTCCACCTTCGGAGACGTGAGGAAGATAATCACCGAGGAGTTC GTCCAGCAGAATTACCTGAAGTACCAGCGTGTGCCCCACATCGAGCCTCCCGAGTACGAGTTCTTCTGGG GGTCCAGAGCTAACCGTGAAATCACCAAGATGCAGATCATGGAGTTCCTGGCCAGAGTCTTCAAGAAAGA TCCCCAGGCGTGGCCTTCCCGATACAGGGAGGCTCTGGAGCAGGCCAGAGCTCTGCGGGAGGCTAATCTT GCTGCCCAGGCCCCCCGCAGCAGTGTCTCTGAGGACTAAAAAGGTCCAGGGGCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4217666), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 9184. Forward Primer - name:ABA_MGC_022_02_G06_F_GGGCTGGGAAGATCTGGT_ABA_FR_022, sequence:CCCTAACTTTGCAGCCGA; Reverse Primer - name:_R_SP6_ABA_MGC_006_02_H12_CCCTAACTTTGCAGCCGAGGTCCC, sequence:GTGCCCCTGGACCTTTTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12498 same embryo
	EMAGE:12497 same embryo
	EMAGE:12495 same embryo
	EurExpress:euxassay_011297 same experiment
	MGI:4826630 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source