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EMAGE:1250

Ncam1 neural cell adhesion molecule 1 ( MGI:97281)
TS13 (8.75 dpc)
immunohistochemistry

Data Images
EMAGE:1250
Fig 2G Bally-Cuif et al, 1993 [PMID:8330525] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations:
Expression Pattern Description
Spatial Annotation:
EMAGE:1250EMAGE:1250Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1250_voxel_strong_3D_1.wlz
1250_voxel_possible_3D_1.wlz
1250_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1250_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
not detected not detected
neural tube
strong strong
regionalImmunoreactivity was strong in the caudal neural tube and neural plate
future brain
not detected not detected
Very weak, diffuse labelling in the head folds. Authors interpret these low, uniform levels of labelling as nonspecific background staining.
future spinal cord neural plate
strong strong
regionalImmunoreactivity was strong in the caudal neural tube and neural plate
trunk somite
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1934203
Entity Detected:Ncam1, neural cell adhesion molecule 1 ( MGI:97281)
Notes:The anti-NCAM1 antibody used in this study by Bally-Cuif et al, 1993 [PMID:8330525] is described as "1/5000-diluted rabbit anti-mouse NCAM antibodies (Gennarini et al., 1984 [PMID:6745267] )". The antisera is in turn described by Gennarini et al as "Rabbit anti-(mouse N-CAM) antibodies", "a gift of G. Edelman and C.-M. Chuong, The Rockefeller University, New York".
Antibody Type:polyclonal
Raised In:rabbit
Supplier:G. Edelman & C.-M. Chuong
Specimen
Organism:mouse
Strain:OF1
Age:8.75 dpc
Theiler Stage:TS13
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection (15% sucrose in 0.12 M phosphate buffer)
Secondary Antibody:sheep anti-rabbit IgG
Labelled with:peroxidase
Visualisation method:diaminobenzidine tetrahydrochloride
General Information
Authors:Bally-Cuif et al, 1993 [PMID:8330525] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8330525] Bally-Cuif L, Goridis C, Santoni MJ 1993 The mouse NCAM gene displays a biphasic expression pattern during neural tube development. Development (117):543-52
 [ doi:10.1111/j.1432-1033.1984.tb08250.x] [ PMID:6745267] Gennarini G, Rougon G, Deagostini-Bazin H, Hirn M, Goridis C 1984 Studies on the transmembrane disposition of the neural cell adhesion molecule N-CAM. A monoclonal antibody recognizing a cytoplasmic domain and evidence for the presence of phosphoserine residues. Eur J Biochem (142):57-64
Links:MGI:1934359 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI