EMAGE mouse gene expression spatial database

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EMAGE:12537

Dctn1 dynactin 1 ( MGI:107745)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011274. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011274_01	euxassay_011274_02	euxassay_011274_03	euxassay_011274_04

euxassay_011274_05	euxassay_011274_06	euxassay_011274_07	euxassay_011274_08	euxassay_011274_09

euxassay_011274_10	euxassay_011274_11	euxassay_011274_12	euxassay_011274_13	euxassay_011274_14

euxassay_011274_15	euxassay_011274_16	euxassay_011274_17	euxassay_011274_18	euxassay_011274_19

euxassay_011274_20	euxassay_011274_21	euxassay_011274_22	euxassay_011274_23	euxassay_011274_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12537_wholemount_strong.wlz

12537_wholemount_moderate.wlz

12537_wholemount_weak.wlz

12537_wholemount_possible.wlz

12537_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12537_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9333
Entity Detected:	Dctn1, dynactin 1 ( MGI:107745)
Sequence:	sense strand is shown >T9333 CGGGCGGCGGCGGGGCCGATCGGGGCGCGGAGCCCTGACAGCAGACGCCGAGTGGCAGCAGGATGAGTAC GGAGGCAAGCGCCCGGCCCCTGCGGGTTGGCTCCCGCGTGGAGGTGATTGGGAAGGGCCACCGAGGCACT GTGGCCTATGTTGGAGCCACACTCTTTGCCACTGGCAAATGGGTGGGCGTGATTCTGGATGAAGCAAAAG GCAAAAATGATGGCACTGTCCAGGGAAGGAAGTATTTCACATGTGATGAAGGCCACGGCATCTTTGTACG CCAGTCCCAGATCCAAGTATTTGAAGATGGAGCAGATACTACTTCCCTAGAGACTCCTGATTCTTCTGCT TCAAAGGTCCTCAAGAGAGAGGGAGCCGATGCAGCTGCAAAGACCAGCAAACTGCGGGGACTGAAGCCTA AGAAGGCACCGACAGCCCGAAAGACCACAACTCGACGGCCCAAGCCTACTCGCCCAGCCAGCACTGGGGT GGCTGGGCCCAGTAGCTCCCTTGGCCCCTCTGGCTCAGCGTCAGCCGGGGAACTAAGCAGCAGTGAGCCC AGCACCCCAGCTCAGACTCCGCTGGCAGCACCCATCATCCCCACACCGGCCCTCACCTCTCCTGGAGCAG CACCCCCACTTCCATCTCCCTCTAAGGAAGAGGAAGGGCTGAGGGCTCAGGTACGGGACCTGGAGGAGAA GCTGGAGACCCTGCGCCTAAAACGCTCAGAAGACAAAGCAAAGCTGAAAGAGCTGGAGAAGCACAAGATC CAGCTGGAGCAGGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCAGACCTGCAGCGGCGCCTCA AGGAGGCTCGGAAGGAAGCCAAGGAGGCGCTAGAGGCAAAGGAACGCTACATGGAGGAGATGGCCGACAC AGCCGACGCTATCGAGATGGCCACTCTGGACAAGGAGATGGCTGAAGAGCGCGCTGAGTCTCTGCAGCAA GAGGTGGAGGCACTGAAGGAACGGGTAGACGAGCTCACCACAGACCTGGAGATTCTCAAGGCTGAAATCG AAGAGAAAGGCTCTGATGGGGCCGCATCAAGCTACCAGCTCAAGCAGCTGGAGGAGCAGAATGCCCGCCT GAAGGATGCCCTGGTGAGGATGCGAGACCTCTCTTCCTCAGAGAAGCAGGAGCACGTGAAGCTGCAGAAA CTCATGGAAAAGAAAAACCAGGAGCTGGAGGTCGTGCGGCAGCAGCGCGAGCGTCTTCAGGAGGAGCTGA GCCAGGCTGAGAGCACCATCGATGAGCTCAAAGAGCAGGTGGACGCCGCTCTGGGAGCCGAGGAGATGGT GGAGATGCTGACCGACCGGAACCTGAATCTAGAGGAGAAAGTGCGGGAGTTACGGGAGACTGTGGGGGAC TTGGAAGCCATGAACGAGATGAACGATGAGCTGCAGGAGAACGCACGGGAGACGGAGCTGGAACTCCGAG AGCAGCTGGACATGGCGGGCGCCCGAGTGAGGGAAGCGCAGAAGCGAGTGGAAGCCGCCCAGGAGACAGT CGCCGACTACCAGCAGACCATCAAGAAGTACCGCCAGTTGACTGCCCACCTACAGGATGTCAATCGGGAG CTGACAAACCAGCAGGAAGCGTCTGTAGAGAGGCAGCAGCAGCCGCCGCCAGAGACTTTTGATTTCAAAA TCAAGTTTGCTGAGACCAAGGCTCATGCCAAGGCCATTGAGATGGAGTTGAGACAGATGGAAGTTGCCCA GGCCAACCGGCACATGTCCCTGCTGACAGCCTTTATGCCTGACAGCTTCCTTCGGCCAGGTGGAGACCAC GACTGTGTCCTGGTGCTGCTGCTCATGCCCCGACTCATTTGCAAGGCAGAGCTCATCCGGAAGCAGGCCC AGGAGAAGTTTGACCTGAGCGAGAACTGTTCGGAGCGGCCCGGGCTGCGGGGAGCTGCCGGGGAGCAGCT GAGCTTTGCTGCTGGACTGGTGTACTCGCTGAGTCTGCTGCAGGCCACGCTGCACCGCTATGAGCATGCC CTCTCTCAGTGCAGTGTGGACGTGTATAAGAAGGTCGGCAGCCTGTACCCCGAGATGAGCGCCCACGAGC GCTCCTTAGATTTCCTCATTGAGCTGCTGCACAAGGATCAGCTGGATGAGACTGTCAACGTGGAGCCCCT CACCAAGGCCATCAAGTATTACCAGCATCTGTACAGCATCCACCTCGCTGAACAACCCGAGGACTCCACC ATGCAGCTGGCTGACCACATCAAGTTCACCCAGAGTGCCCTGGACTGCATGGGCGTGGAGGTGGGGCGGC TGCGTGCCTTCTTGCAGGGTGGGCAGGAGGCAACAGATATTGCCCTTCTTCTCCGAGACCTGGAAACATC ATGTAGTGACACCCGTCAGTTCTGCAAGAAGATCCGAAGGCGGATGCCGGGGACGGATGCTCCTGGGATC CCAGCAGCGCTGGCCTTTGGCTCACAGGTATCCGACACACTCCTGGACTGCAGGAAGCACTTGACGTGGG TGGTAGCTGTTCTGCAGGAGGTGGCAGCTGCAGCCGCCCAGCTTATTGCCCCCTTGGCAGAGAACGAGGG GCTGCCTGTGGCTGCACTGGAGGAGCTGGCCTTCAAAGCAAGCGAGCAGATCTACGGGAGCCCCTCCAGC AGCCCCTATGAGTGTCTACGCCAGTCATGCACCATCCTCATCAGCACGATGAACAAGCTGGCCACAGCCA TGCAAGAAGGCGAGTATGACGCAGAGCGACCCCCGAGCAAGCCTCCTCCGGTTGAACTTCGGGCTGCAGC CCTGCGTGCAGAGATCACAGATGCTGAAGGTCTGGGTTTGAAGCTTGAGGATCGAGAGACAGTTATCAAG GAGTTAAAGAAGTCACTCAAGATTAAGGGAGAGGAGCTGAGTGAGGCCAACGTGCGGCTCAGCCTCCTGG AGAAGAAGTTGGACAGCGCTGCCAAGGATGCAGACGAGCGAATCGAGAAAGTTCAGACACGGCTGGACGA GACTCAGACCCTGCTGCGGAAGAAGGAGAAAGACTTTGAGGAGACAATGGACGCACTCCAGGCTGACATC GACCAGCTGGAGGCAGAGAAGGCAGAGCTCAAGCAGCGCCTGAACAGCCAGTCCAAGCGCACAATCGAGG GGCTCCGGGGCCCCCCTCCGTCAGGCATCGCTACCCTGGTCTCTGGCATCGCTGGTGAGGAACCACAGCG AGGGGGCGCTCCTGGGCAGGCTCCGGGCGCCTTGCCAGGCCCGGGGCTGGTGAAGGACTCCCCACTGCTG CTTCAGCAGATCTCTGCTATGAGGCTACACATCTCTCAGCTCCAGCATGAGAACAGCATCCTCAGAGGAG CCCAGATGAAGGCGTCCTTGGCAGCTCTGCCCCCTCTGCATGTTGCAAAGCTTTCCCTCCCACCCCATGA GGGCCCCGGTGGTAACCTAGTGGCTGGGGCACTGTACCGCAAGACCAGCCAGCTCCTGGAGAAACTAAAC CAGCTGAGTACCCACACCCACGTGGTGGATATCACTCGGAGCAGCCCAGCTGCCAAGAGCCCGTCAGCTC AGCTTATGGAACAAGTGGCTCAGCTCAAGTCCCTGAGTGACACCATTGAGAAGCTCAAGGATGAGGTCCT CAAGGAGACAGTGACTCAGCGCCCTGGAGCCACTGTGCCCACCGACTTTGCCACTTTCCCTTCATCTGCC TTCCTCAGGGCCAAGGAAGAGCAGCAAGATGACACAGTCTACATGGGCAAAGTGACCTTTTCATGCGCGG CAGGCCTAGGACAGCGACACCGCCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGTCGCCTCAT CTCCTAAGCACTCCTTCCCTGCCTTCCCTGTGGTCCCACTGCGCAGGGCACAGCCACCTCAGCCACTCCC AGGCTGGACCACACGCTAACCTCCTGAGTGGTTCAGCTGCGTCTGGTCTGGTCAGGGTCCTGCTGCTTCC GTAACCTGGGTTTGCCTGTCCACCCCTGGCCTCTTCCGTATTTGCTGTTATCTGCTCCCTCTTTCCTGAG GGGCCTCAGGGCATTGGAGGGGGCCGACACCCCTCCACTAAAGATTGAGTGAGGTCTCCCTGACTGAGGA CTTCACCCCTTGATTAAAGCAACTTGTGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6849060 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6849060 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12536 same embryo
	EurExpress:euxassay_011274 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source