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EMAGE:1254

Sox10 SRY-box containing gene 10 ( MGI:98358)
TS16 (10 dpc)
in situ hybridisation

Data Images
EMAGE:1254
Figure 6A of Kokubu et al., 2004 [PMID:15469977] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: HL - hindlimb bud.
Expression Pattern Description
Spatial Annotation:
EMAGE:1254Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1254_wholemount_strong_3D_1.wlz
1254_wholemount_moderate_3D_1.wlz
1254_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1254_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
detected detected
single cellSox10 marks cell in the Dorsal Root Ganglion of Neural Crest origin.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3055733
Entity Detected:Sox10, SRY-box containing gene 10 ( MGI:98358)
Sequence:sense strand is shown

>MGI:3055733
CCATGGCCCTCCTACCCCTCCAACCACCCCAAAGACAGAGCTGCAGTCCGGCAAGGCAGATCCCAAAAGG
GATGGGCGCTCCTTGGGGGAGGGCGGGAAGCCCCACATCGACTTCGGCAATGTGGACATCGGGGAGATCA
GCCATGAGGTAATGTCCAACATGGAGACCTTTGATGTGACTGAGCTGGACCAATACCTGCCGCCCAATGG
GCACCCAGGCCACGTGGGCAGCTACTCGGCAGCTGGCTACGGGCTGAGCAGTGCCCTGGCTGTGGCCAGT
GGACACTCTGCCTGGATCTCCAAGCCACCAGGTGTGGCTCTGCCCACGGTCTCGCCCCCTGCTGTGGATG
CCAAAGCCCAGGTGAAGACAGAGACCACAGGGCCCCAGGGACCCCCACACTACACCGACCAGCCGTCCAC
TTCCCAGATCGCCTACACCTCCCTCAGTCTGCCCCACTACGGCTCCGCCTTCCCCTCCATCTCACGCCCC
CAGTTTGACTACTCTGACCATCAGCCCTCAGGACCCTATTATGGCCACGCAGGCCAGGCCTCTGGCCTCT
ACTCAGCTTTTTCCTACATGGGCCCCTCTCAGCGGCCCCTCTACACGGCCATCTCTGACCCCAGCCCCTC
AGGGCCCCAGTCCCACAGTCCCACACACTGGGAACAGCCAGTATATACGACTCTATCCCGACCTTAGAGG
GGCCCTGTCACCATTGGTGCCCACGTGGCCCAGGGCCCCTTCCCCCAGCCTGTGTGCCCTGCTCCTCATC
AGCCTCATGCCCAGCAGTGGCAGTGGAGGAGGCTGCAGAGGCTGACAGCAGCAGGCAGGCTTCTGCCAGC
TCCTGCCCTGATGACCTCCACCCACCCTGGCTCGGGAGGCCAAGCCCTGACTGAGCTGGCAAAGGAGGAG
TTTGGTGGCCATCCCAGACTGAGGGTGAGGGGCCACCCATCCCCAGAAATAACCCTCTATCCCAGGACCT
GAGAAGTCGTTCTCAAGTCTTTCAGGGAGGCAGAAAAGGGTATGGTGGGAAACCTCACTGCTCCTGTTCT
CTCCTGGGAGAGCAAGGGGCTCAAGGGCCTGTGTGCCACTGTGTCACTGTGCCTAAAAGCTAGGCTGCCT
AGGGACCTGCTTCAAAGCCTGGAGCTGGCTCTGTCCTGTCACTAAGGACTCACTGAGGACAGCTTTGAAC
AGCTTTGTGAAGAGCAGGGTGGGACACTCAATGGACACTCTGATCCTTTCACCCCCCTCCTCACCTGAAT
GGGAACTGGCCGAGGTCCCCAGATCCAGTTCTGCATTAGTAACCTCATCCCTCACCTAACTGAGGAGAGT
CCTCATGTTCTTCCCCACGACCTCCACTCCTCAAGGCCCACCCCAGGTGAGGAGGCAGGGGCTCCAGGAA
AGAATCAGAGGTGTCCAGCCTTCCTCTCGCCTTCTGTCAGCCTCAGTTAGCACTGCACGGGCCCTAGCCC
CAGAGCCCAGTACCTGGGTTGCTGGCAGCAGGCTGGACACTAAACCCCTTCCATGTACAGTCTACCTTTG
CCTCGCACCCTTTGCTCCAGCGATATCTTAATAAAGTACGTAGCTCTGTCTCCCCATCTCCTGTCTGCAG
CCCAGTCCACACGTAACACATCGCTGCCCCTTTATCTCCACAGTCACCATGTTCCCATCCCTCTAGCTAC
TCCAGCCCCAATTAACTTGCATTAAGCACTCCAAGTAATAAAATCAAAGCTTCTGGTTGCCTGC
nt 1287 - nt 3030 of AJ001029.1
Notes:The Sox10 probe used in this study by Kokubu et al., 2004 [PMID:15469977] was previously described by Kuhlbrodt et al., 2004 [PMID:9412504] as follows: "the 1.75 kb antisense riboprobe was transcribed by SP6 polymerase after linearizing pZL1/Sox10 with NcoI and corresponded to the second half of the Sox10 cDNA without the HMG domain."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:BALB/c
Age:10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Kokubu et al., 2004 [PMID:15469977] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.01405] [ PMID:15469977] Kokubu C, Heinzmann U, Kokubu T, Sakai N, Kubota T, Kawai M, Wahl MB, Galceran J, Grosschedl R, Ozono K, Imai K 2004 Skeletal defects in ringelschwanz mutant mice reveal that Lrp6 is required for proper somitogenesis and osteogenesis. Development (131):5469-80
 [ PMID:9412504] Kuhlbrodt K, Herbarth B, Sock E, Hermans-Borgmeyer I, Wegner M 1998 Sox10, a novel transcriptional modulator in glial cells. J Neurosci (18):237-50
Links:MGI:3055734 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI