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EMAGE:12554

Vldlr very low density lipoprotein receptor ( MGI:98935)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554
"Pseudo-wholemount" of euxassay_011409. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011409_01 euxassay_011409_02 euxassay_011409_03 euxassay_011409_04
EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554
euxassay_011409_05 euxassay_011409_06 euxassay_011409_07 euxassay_011409_08 euxassay_011409_09
EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554
euxassay_011409_10 euxassay_011409_11 euxassay_011409_12 euxassay_011409_13 euxassay_011409_14
EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554
euxassay_011409_15 euxassay_011409_16 euxassay_011409_17 euxassay_011409_18 euxassay_011409_19
EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554 EMAGE:12554
euxassay_011409_20 euxassay_011409_21 euxassay_011409_22 euxassay_011409_23 euxassay_011409_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12554Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12554_wholemount_strong.wlz
12554_wholemount_moderate.wlz
12554_wholemount_weak.wlz
12554_wholemount_possible.wlz
12554_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12554_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 21 22 23 moderate expression: see section 08 18 19 20 24
cerebral cortex marginal layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 21 22 23 24 weak expression: see section 09 10 11 12 17 18 19 20
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 13 17 weak expression: see section 12 18
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 05 17 weak expression: see section 06 07 10 16
rest of cerebellum marginal layer
weak weak
regionalweak expression: see section 06 07 08 14 15
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 weak expression: see section 17
ventral grey horn
strong strong
regionalstrong expression: see section 13 14
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 04 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18 weak expression: see section 10 19
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 13 16
mandible
strong strong
regionalstrong expression: see section 03 04 05 06 09 10 23 moderate expression: see section 07 08 11 12 13 18 19 20 21 22
lower jaw incisor
strong strong
regionalstrong expression: see section 17 moderate expression: see section 13 14 18
maxilla
strong strong
regionalstrong expression: see section 09 10 moderate expression: see section 07 08 11 12 13 18 19 20 21 22
upper jaw incisor
strong strong
regionalstrong expression: see section 17 moderate expression: see section 13 14 18
bladder
weak weak
regionalweak expression: see section 13 14 15
ureter
weak weak
regionalweak expression: see section 12 15
renal cortex
strong strong
regionalstrong expression: see section 06 07 08 09 10 16 17 18 19 20 21
male reproductive system
moderate moderate
regionalmoderate expression: see section 13 14 15 weak expression: see section 12
orbito-sphenoid
strong strong
regionalstrong expression: see section 03 04 05 moderate expression: see section 01 02 23 24
clavicle
strong strong
regionalstrong expression: see section 09 10 17 moderate expression: see section 18 weak expression: see section 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30096
Entity Detected:Vldlr, very low density lipoprotein receptor ( MGI:98935)
Sequence:sense strand is shown

>T30096
AGCTGTATCCACGGCAGCAGGCAATGCAATGGCATCCGAGACTGTGTTGATGGCTCTGATGAAGTTAACT
GCAAAAACGTCAATCAGTGCCTGGGCCCTGGAAAGTTCAAGTGCAGAAGCGGGGAATGCATAGACATGAG
CAAAGTATGTGACCAGGAACAAGACTGCAGAGACTGGAGTGACGAGCCCCTGAAGGAATGCCATATCAAC
GAATGCCTGGTCAATAATGGTGGCTGTTCCCATATCTGCAAAGACCTAGTTATAGGTTATGAGTGTGATT
GTGCAGCTGGGTTTGAACTGATAGATAGGAAAACCTGTGGAGATATTGATGAATGCCAAAACCCGGGGAT
CTGCAGTCAAATTTGTATCAACTTAAAAGGCGGTTACAAGTGTGAATGTAGTCGTGGCTATCAAATGGAT
CTTGCCACTGGCGTGTGCAAGGCAGTAGGCAAAGAGCCGAGTCTGATCTTCACTAATCGAAGAGACATCA
GGAAGATTGGCCTAGAGAGAAAGGAATACATCCAACTTGTAGAGCAACTAAGGAACACGGTGGCTCTCGA
TGCGGACATTGCAGCTCAGAAGCTGTTTTGGGCTGATCTCAGCCAGAAGGCCATCTTCAGTGCCTCAATT
GATGACAAGGTTGGTAGACATTTTAAAATGATCGACAATGTCTATAATCCTGCAGCCATTGCTGTTGATT
GGGTGTACAAGACCATCTACTGGACTGATGCGGCTTCTAAGACTATTTCAGTAGCTACCCTAGACGGAGC
CAAGAGGAAGTTCCTGTTTAATTCTGACTTGCGAGAGCCTGCCTCCAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3968213), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15310. Forward Primer - name:015310_F_IRAV20-23_M09_Vldlr, sequence:AGCTGTATCCACGGCAGC; Reverse Primer - name:015310_R_SP6_IRAV20-23_M09_Vldlr, sequence:TATGGAGGCAGGCTCTCG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12557 same embryo
 EMAGE:12555 same embryo
 EMAGE:12556 same embryo
 EurExpress:euxassay_011409 same experiment
 MGI:4829164 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS