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EMAGE:1256

Vcam1 vascular cell adhesion molecule 1 ( MGI:98926)
TS12 (8.0 dpc)
immunohistochemistry

Data Images
EMAGE:1256
Figure 3J of Naiche & Papaioannou, 2003 [PMID:12736212] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Arrowhead indicates the base of the allantois.
Expression Pattern Description
Spatial Annotation:
EMAGE:1256Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1256_wholemount_strong_3D_1.wlz
1256_wholemount_moderate_3D_1.wlz
1256_wholemount_weak_3D_1.wlz
1256_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1256_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
extraembryonic component
detected detected
regionalExpression in distal tip of (fused) allantois.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2676334
Entity Detected:Vcam1, vascular cell adhesion molecule 1 ( MGI:98926)
Notes:The anti-VCAM1 antibody used in this study by Naiche & Papaioannou, 2003 [PMID:12736212] is described as follows, "anti-VCAM1 (PharMingen, catalog number 55333O". The Pharmingen Technical Data sheet states this "antibody reacts with both the long (~110 kDa) transmembrane-spanning form and the truncated (~47 kDa) GPI-linked form of vascular cell adhesion molecule-1 (VCAM-1, CD106)."
Antibody Type:monoclonal
Raised In:rat
Supplier:PharMingen
Catalogue Number:553330
Specimen
Organism:mouse
Age:8.0 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Secondary Antibody:goat anti-rat IgG
Labelled with:peroxidase
General Information
Authors:Naiche & Papaioannou, 2003 [PMID:12736212] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00504] [ PMID:12736212] Naiche LA, Papaioannou VE 2003 Loss of Tbx4 blocks hindlimb development and affects vascularization and fusion of the allantois. Development (130):2681-93
Links:MGI:2677417 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI