EMAGE mouse gene expression spatial database

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EMAGE:12569

Gdap1l1 ganglioside-induced differentiation-associated protein 1-like 1 ( MGI:2385163)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011524. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011524_01	euxassay_011524_02	euxassay_011524_03	euxassay_011524_04

euxassay_011524_05	euxassay_011524_06	euxassay_011524_07	euxassay_011524_08	euxassay_011524_09

euxassay_011524_10	euxassay_011524_11	euxassay_011524_12	euxassay_011524_13	euxassay_011524_14

euxassay_011524_15	euxassay_011524_16	euxassay_011524_17	euxassay_011524_18	euxassay_011524_19

euxassay_011524_20	euxassay_011524_21	euxassay_011524_22	euxassay_011524_23	euxassay_011524_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12569_wholemount_strong.wlz

12569_wholemount_moderate.wlz

12569_wholemount_weak.wlz

12569_wholemount_possible.wlz

12569_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12569_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 08 09 22 23 24

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 21

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 09 10 20

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 10 20 21 22

trigeminal v nerve

strong

regional

strong expression: see section 12 20

spinal cord

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19

cervico-thoracic ganglion

strong

regional

strong expression: see section 13 19 20 moderate expression: see section 12

cervical ganglion

strong

regional

strong expression: see section 19 20 moderate expression: see section 11

thoracic ganglion

strong

regional

strong expression: see section 14 15 16 17 18

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 18 19 20

neural retina

strong

regional

strong expression: see section 05 06 moderate expression: see section 02 03 04

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 14 15 17 18 19 20 21 22 23 moderate expression: see section 11 12 13 16

vomeronasal organ

strong

regional

strong expression: see section 15 18

tongue

moderate

spotted

moderate expression: see section 15 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30978
Entity Detected:	Gdap1l1, ganglioside-induced differentiation-associated protein 1-like 1 ( MGI:2385163)
Sequence:	sense strand is shown >T30978 GCCTGGCCTGTGAAGAGAGAGATGTGAGCCTGCCTCAGAGCGAGCATAAGGAACCGTGGTTCATGCGGCT CAACCTGGGTGAGGAGGTCCCAGTCATTATCCACAGAGACAACATTATCAGTGACTATGACCAGATCATT GACTACGTGGAACGCACCTTCACGGGAGAGCATGTGGTGGCCTTGATGCCCGAGGCGGGCAGCCCACAAC ACGCCCGAGTGCTGCAGTACCGGGAGCTGCTGGATGCGCTGCCCATGGACGCCTACACACACGGCTGCAT CCTGCACCCTGAGCTCACCACAGATTCCATGATCCCCAAGTATGCCACGGCTGAGATCCGCAGACATTTA GCCAACGCCACCACAGACCTCATGAAACTGGACCACGAGGAGGAACCACAGCTCTCTGAGCCCTACCTTT CTAAACAAAAGAAGCTTATGGCCAAGATCCTGGAGCACGATGACGTGAGCTACCTGAAGAAGATCCTCGG GGAGCTGGCTATGGTGCTGGACCAGATTGAGGCGGAGCTGGAGAAGAGGAAACTGGAGAATGAGGGGCAG ACGTGCGAGCTGTGGCTCTGTGGCTGTGCCTTCACCCTGGCGGATGTCCTCCTGGGAGCCACCCTGCACC GCCTCAAGTTCCTGGGACTGTCCAAGAAATACTGGGAAGACGGCAGCCGGCCCAACCTGCAGTCCTTCTT TGAGAGGGTCCAGAGACGCTTTGCCTTCCGGAAAGTCCTGGGCGACATCCACACCACTCTGCTGTCAGCT GTTATCCCCAACGCGTTCCGACTGGTCAAACGGAAACCGCCATCCTTCTTTGGGGCATCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3969548), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21836. Forward Primer - name:021836_F_IRAV32-35_A06_Gdap1l, sequence:GCCTGGCCTGTGAAGAGA; Reverse Primer - name:021836_R_SP6_IRAV32-35_A06_Gdap1l, sequence:AGGATGCCCCAAAGAAGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12571 same embryo
	EMAGE:12568 same embryo
	EMAGE:12572 same embryo
	EMAGE:12570 same embryo
	EurExpress:euxassay_011524 same experiment
	MGI:4825029 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source