EMAGE mouse gene expression spatial database

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EMAGE:12583

9430007A20Rik RIKEN cDNA 9430007A20 gene ( MGI:2685880)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011336. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011336_01	euxassay_011336_02	euxassay_011336_03	euxassay_011336_04

euxassay_011336_05	euxassay_011336_06	euxassay_011336_07	euxassay_011336_08	euxassay_011336_09

euxassay_011336_10	euxassay_011336_11	euxassay_011336_12	euxassay_011336_13	euxassay_011336_14

euxassay_011336_15	euxassay_011336_16	euxassay_011336_17	euxassay_011336_18	euxassay_011336_19

euxassay_011336_20	euxassay_011336_21	euxassay_011336_22	euxassay_011336_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12583_wholemount_strong.wlz

12583_wholemount_moderate.wlz

12583_wholemount_weak.wlz

12583_wholemount_possible.wlz

12583_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12583_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord mantle layer

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37478
Entity Detected:	9430007A20Rik, RIKEN cDNA 9430007A20 gene ( MGI:2685880)
Sequence:	sense strand is shown >T37478 TGGGTTCTCATAGACCACTTCCTTACGATTGATGTGCCTCCTTCCATTCCACACCCTGTGAAGTTTCGGA TTCTACACTTCTGCTTTCATCTGACAACCACATGGGGGCATATACTCGAGAAGATGAACATTTGCTCCAT GCCTCAATTTTTCTGCTTTTTACAAGACAGCCTTTCATCAAAGGAGAACCATGGTGTGTTTGTGAAGGAC CTGCGTTTTGGCACAATCCCTGTAAGACTCTTTCGTCCCAAGGCAGCCTCCTCCAAACCCCGACGAGGCA TCCTCTTTTTCCATGGAGGAGGAGCAATGATTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 72617. Forward Primer - name:072617_F_cDNA_9430007A20Rik, sequence:TGGGTTCTCATAGACCACTTCC; Reverse Primer - name:072617_N_SP6_cDNA_9430007A20Rik, sequence:CAATCATTGCTCCTCCTCCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12582 same embryo
	EMAGE:12584 same embryo
	EurExpress:euxassay_011336 same experiment
	MGI:4822803 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source