EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12587

Lhfpl3 lipoma HMGIC fusion partner-like 3 ( MGI:1925076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011531. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011531_01	euxassay_011531_02	euxassay_011531_03	euxassay_011531_04

euxassay_011531_05	euxassay_011531_06	euxassay_011531_07	euxassay_011531_08	euxassay_011531_09

euxassay_011531_10	euxassay_011531_11	euxassay_011531_12	euxassay_011531_13	euxassay_011531_14

euxassay_011531_15	euxassay_011531_16	euxassay_011531_17	euxassay_011531_18	euxassay_011531_19

euxassay_011531_20	euxassay_011531_21	euxassay_011531_22	euxassay_011531_23	euxassay_011531_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12587_wholemount_strong.wlz

12587_wholemount_moderate.wlz

12587_wholemount_weak.wlz

12587_wholemount_possible.wlz

12587_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12587_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 23 24

olfactory cortex mantle layer

weak

regional

weak expression: see section 11 12 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37530
Entity Detected:	Lhfpl3, lipoma HMGIC fusion partner-like 3 ( MGI:1925076)
Sequence:	sense strand is shown >T37530 TCCTTGATCCCTCATTGCTACTGTCCACTCATGTCACTGTGCAATTAGTTACAACCACGTGGTTATTAAG AGACTTAGCCCTTCAAACTTCTTACCCAATGTGTGTTCAGTTTAATCTGTTTGTACGACTTATCACGAAG GAAATGTTTCTGCCATGTGCTATGACTCCATCAAACTGTGAATCACAGGAAGTACATCCAGACATCGTAG GTTCCCAAGCAACATAGATTCCCCTGTCATTGACAAAACAGCATCAAGGAGCTCTGAACAATATAGAAAG TTTATTTTCACTTGGATGCTCTCTCATGTAATATATAGGCTACTATCAAATAAACGCTTTTTCTAATTCT CCCTAGTATATGCATAGGAATTTAATATACTTTATAAATAGATGTCTAAAATATGAGTTTTCTATTTCTT CATTCTATATGTCACTAGAAATCCACATTCTTTATTTCCTTTACTGACATATACTCATTTTTGTTTTGTT AAAAGAAACTATTCCATTAAATCTACTTCTAAATGATAGTAAGTGGTACTAACAAAATAAGTAAAAACTT AATAGCCTTAGAAGTAAATAACTATATACTTATATGTCAAAGGAACTTGGTAGGAATAAGTTACCTTTTG TTTATTAATGTTGATTTTGAAATTTCTCATATTTTTTCCCTTTTGGTTAGCTGGTACTTGAAAGTAGTTA CAACTAACTAGCTGTCTTCAAGAATCTTTTCCTTCCAGGGTCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 78583. Forward Primer - name:078583_F_cDNA_A930031L14Rik, sequence:TCCTTGATCCCTCATTGCTACT; Reverse Primer - name:078583_N_SP6_cDNA_A930031L14Rik, sequence:GGGACCCTGGAAGGAAAAGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12586 same embryo
	EMAGE:12590 same embryo
	EMAGE:12589 same embryo
	EMAGE:12588 same embryo
	EMAGE:12585 same embryo
	EurExpress:euxassay_011531 same experiment
	MGI:4825919 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source