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EMAGE:12604

Usp7 ubiquitin specific peptidase 7 ( MGI:2182061)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12604 EMAGE:12604 EMAGE:12604 EMAGE:12604 EMAGE:12604
"Pseudo-wholemount" of euxassay_017481. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017481_01 euxassay_017481_02 euxassay_017481_03 euxassay_017481_04
EMAGE:12604 EMAGE:12604 EMAGE:12604 EMAGE:12604 EMAGE:12604
euxassay_017481_05 euxassay_017481_06 euxassay_017481_07 euxassay_017481_08 euxassay_017481_09
EMAGE:12604 EMAGE:12604 EMAGE:12604 EMAGE:12604 EMAGE:12604
euxassay_017481_10 euxassay_017481_11 euxassay_017481_12 euxassay_017481_13 euxassay_017481_14
EMAGE:12604 EMAGE:12604 EMAGE:12604 EMAGE:12604 EMAGE:12604
euxassay_017481_15 euxassay_017481_16 euxassay_017481_17 euxassay_017481_18 euxassay_017481_19
EMAGE:12604 EMAGE:12604 EMAGE:12604 EMAGE:12604
euxassay_017481_20 euxassay_017481_21 euxassay_017481_22 euxassay_017481_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12604Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12604_wholemount_strong.wlz
12604_wholemount_moderate.wlz
12604_wholemount_weak.wlz
12604_wholemount_possible.wlz
12604_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12604_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39610
Entity Detected:Usp7, ubiquitin specific peptidase 7 ( MGI:2182061)
Sequence:sense strand is shown

>T39610
GCTGCCCACTGCTAAAGAGTATTTCAGAGATCTCTACCACCGGGTCGACGTCATCTTCTGTGACAAAACG
ATCCCCAATGACCCTGGATTTGTGGTCACATTATCAAATAGGATGAACTATTTTCAGGTTGCAAAGACAG
TTGCACAGAGGCTCAACACAGACCCCATGCTGCTGCAGTTTTTCAAGTCTCAAGGTTATAGGGACGGCCC
CGGTAATCCTCTTAGGCATAATTACGAAGGTACTTTAAGAGATCTTCTACAATTCTTCAAGCCAAGACAA
CCTAAGAAACTTTACTATCAGCAACTTAAGATGAAAATCACAGACTTTGAGAACAGGAGAAGTTTTAAGT
GTATATGGTTAAACAGCCAATTTAGGGAAGAGGAAATAACACTATACCCAGACAAGCATGGCTGTGTCCG
GGACCTGTTAGAAGAATGTAAAAAAGCCGTGGAGCTCGGTGACAAAGCATCAGGGAGACTTAGGCAAGCT
GCTAGAAATTGTGAGCTACAAAATTATTGGTGTGCATCAAGAAGATGAACTATTAGAATGCTTATCTCCT
GCAACGAGTCGGACTTTTAGAATAGAGGAAATACCTTTGGACCAGGTAGACATAGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 161692. Forward Primer - name:161692_F_cDNA_mCG123721.1, sequence:GCTGCCCACTGCTAAAGAGTAT; Reverse Primer - name:161692_N_SP6_cDNA_mCG123721.1, sequence:TCTATGTCTACCTGGTCCAAAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12603 same embryo
 EurExpress:euxassay_017481 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS