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EMAGE:12608

Pyy peptide YY ( MGI:99924)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12608 EMAGE:12608 EMAGE:12608 EMAGE:12608 EMAGE:12608
"Pseudo-wholemount" of euxassay_011430. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011430_01 euxassay_011430_02 euxassay_011430_03 euxassay_011430_04
EMAGE:12608 EMAGE:12608 EMAGE:12608 EMAGE:12608 EMAGE:12608
euxassay_011430_05 euxassay_011430_06 euxassay_011430_07 euxassay_011430_08 euxassay_011430_09
EMAGE:12608 EMAGE:12608 EMAGE:12608 EMAGE:12608 EMAGE:12608
euxassay_011430_10 euxassay_011430_11 euxassay_011430_12 euxassay_011430_13 euxassay_011430_14
EMAGE:12608 EMAGE:12608 EMAGE:12608 EMAGE:12608 EMAGE:12608
euxassay_011430_15 euxassay_011430_16 euxassay_011430_17 euxassay_011430_18 euxassay_011430_19
EMAGE:12608 EMAGE:12608 EMAGE:12608 EMAGE:12608
euxassay_011430_20 euxassay_011430_21 euxassay_011430_22 euxassay_011430_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12608Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12608_wholemount_strong.wlz
12608_wholemount_moderate.wlz
12608_wholemount_weak.wlz
12608_wholemount_possible.wlz
12608_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12608_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
pancreas
strong strong
regionalstrong expression: see section 10 11 12 15 16
medulla oblongata basal plate mantle layer
strong strong
single cellstrong expression: see section 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30129
Entity Detected:Pyy, peptide YY ( MGI:99924)
Sequence:sense strand is shown

>T30129
TAGCTGCTATGGTGGCGGTGCGCAGGCCTTGGCCCGTCACGGTCGCAATGCTGCTAATCCTGCTCGCCTG
TCTGGGAGCCCTGGTGGACGCCTACCCTGCCAAACCAGAGGCTCCCGGCGAAGACGCCTCCCCGGAGGAG
CTGAGCCGCTACTACGCCTCCCTGCGCCACTACCTCAACCTGGTCACCCGGCAGCGGTATGGAAAAAGAG
ATGTCCCCGCAGCTCTGTTCTCCAAACTGCTCTTCACAGACGACAGCGACAGCGAGAACCTCCCCTTCAG
GCCAGAAGGTTTGGACCAGTGGTGAAGACTCCCCCAAGGCCTCCTGCGAGATGTGTTAACTACACCGACT
TCACTTGCATGTTTGGTTTAAGAAGAGGGCACTTCATATCTCGGTGTCTCGGACACCCAGACTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4218355), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 19251. Forward Primer - name:019251_F_IRAV28-31_G04_Pyy, sequence:TAGCTGCTATGGTGGCGG; Reverse Primer - name:019251_R_SP6_IRAV28-31_G04_Pyy, sequence:CCAGTCTGGGTGTCCGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12612 same embryo
 EMAGE:12610 same embryo
 EMAGE:12611 same embryo
 EMAGE:12609 same embryo
 EurExpress:euxassay_011430 same experiment
 MGI:4827554 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS